Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP000730	1,747,382	(TAC)3→2	95.3%	coding (2065‑2067/2280 nt)	secDF ←	RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	1747377 =	2 (0.030)	39 (0.650)	31/242	NT	95.3%	coding (2072/2280 nt)	secDF	RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter
	?	CP000730	= 1747373	2 (0.030)					coding (2076/2280 nt)	secDF	RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter

AACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/1747517‑1747377 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtagtagtTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAAAAA  <  CP000730/1747373‑1747235                                                                                                                                                                                                                                                                                          AACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTACCAGTTCTTTTAGTAG                                                                                                                                              >  2:139350/1‑140   CGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAGTACAGAATTAAAAGTTCTTGTAGTAGTT                                                                                                                                            >  2:748100/1‑140   CGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTATAGTAttg                                                                                                                                            >  2:634932/1‑137    GTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATCTCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTG                                                                                                                                           <  2:5260/140‑1        tACCTTGTACGTGAAAACTTAGAAAAGGTTAAATTTATTACGACAACAGAACAAATTGATGATATGGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTAT                                                                                                                                       <  1:530638/139‑1         ACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTACTAACAGTTATTGTAGTACTTGCTATA                                                                                                                                      >  2:703350/1‑140          CCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAAAAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATAC                                                                                                                                     <  1:576729/140‑1            ggGTACGTGAAAACCTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAAACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTA                                                                                                                                   <  2:677381/138‑1                ACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACAGTCAATTAATACAGTTTTAACAATTATTGTCGTAGATGCGATACTATTCT                                                                                                                               >  2:433124/1‑140                    GAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACGAATTGGTGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGG                                                                                                                           >  2:499090/1‑140                    GAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCAATACTATTCTTCGG                                                                                                                           >  1:651009/1‑140                    GAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGAAAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGGTATACTATTCTTAGG                                                                                                                           >  1:216233/1‑140                     AAAACTTAAAAAAGGTTATAGTGATTAAGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGT                                                                                                                          <  1:655493/140‑1                                   GTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACTCGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCAAAACTATTCTTCAATGCTCATACGATATT                                                                                                            >  1:305394/1‑140                                   GTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATT                                                                                                            >  1:538673/1‑140                                   GTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATT                                                                                                            >  2:320422/1‑140                                   GTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATT                                                                                                            >  2:490300/1‑140                                    TTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTC                                                                                                           >  1:303659/1‑140                                        AGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCACCT                                                                                                       >  2:669251/1‑140                                           GATTACGACAACAGAACAAATTGATGATATCGTTAATAAAGCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTGCGATATTCAACCTTA                                                                                                    >  2:192496/1‑140                                             TTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACT                                                                                                  >  1:303791/1‑140                                             TTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGATATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACT                                                                                                  >  2:209807/1‑140                                                   CAACAGAACAAATTGATTATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACATTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCA                                                                                            <  1:478795/140‑1                                                       aggACAAATTGATGAAATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTAT                                                                                        <  1:125105/137‑1                                                                TGATGATATCGTTAATAGATCCATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGAT                                                                               <  2:6015/140‑1                                                                 GATGATATCGTTAATAGATCAACTAGACAGACAAATACACGTTCAATTAATACAGTAGGAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATT                                                                              >  2:536640/1‑140                                                                  ATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTG                                                                             <  2:628176/140‑1                                                                  ATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGATGCTCCTACGATATTCAACTTTAATTTAGCAATATTTATCGGATTa                                                                             >  1:584416/1‑139                                                                   TGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACCGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGATCCTAAGATATTCAACTTTACTTTAGCATTATTTATCGGATTGA                                                                            >  1:223541/1‑140                                                                    GATATCGTTAATAGATCAATTCGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGACATTCAACTTTACTTTAGCATTATTTATCGGATTGAT                                                                           <  1:526070/140‑1                                                                    GATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATAGGATTGAT                                                                           >  1:724374/1‑140                                                                               TAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTAT                                                                <  1:634932/140‑1                                                                                AGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTTATTTCTGGTGTATT                                                               >  1:543499/1‑140                                                                                           ACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATAGGATTGATTTCTGGTGTATTTTCTTAAATCT                                                    >  1:261633/1‑140                                                                                           ACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGAATTATTTATCAAATTGATTTCTGGTGTATTCTCTTCAATtt                                                    >  1:50717/1‑138                                                                                               ACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCAT                                                >  2:646007/1‑140                                                                                                       ACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTC                                        <  1:349604/140‑1                                                                                                                 AATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCGACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGG                              <  1:351381/140‑1                                                                                                                   TACAGTATTAAGAGTTATTGTAGTAGTTGTTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAA                            <  2:255971/140‑1                                                                                                                       GTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCAGATTGATTTCTGGTGAATTCTCTTCAATCTTAATTGACGTTGCGCTAGGGGAAAAAAT                        >  1:6183/1‑140                                                                                                                          TTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACCTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAA                     <  1:192496/140‑1                                                                                                                             ACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGGAAAA                  >  2:436278/1‑140                                                                                                                             ACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTGTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCCTTGCCGTTCCGCTATGGGGAATACTGAAAca                  >  2:331622/1‑138                                                                                                                                          TAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACGTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAA     <  2:86082/140‑1                                                                                                                                          TAGTTGCTATACTATGCTTCGGTGCTCCTACGATATTCAAGTTCACTTTAGCATTATTTATCGGATTGAGTTCTGCTGTATTCTCTTCAATCCTCTTTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAA     <  2:271357/140‑1                                                                                                                                             TTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAAAAA  <  1:87887/140‑1                                                                                                                                                                                                                                                                                          AACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/1747517‑1747377 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtagtagtTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAAAAA  <  CP000730/1747373‑1747235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.