New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 539419	35 (1.020)	5 (0.150)	4/262	4.9	13.1%	coding (1730/3507 nt)	mfd	transcription‑repair coupling factor helicase
	?	CP000730	= 539422	33 (1.020)					coding (1733/3507 nt)	mfd	transcription‑repair coupling factor helicase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAGGTACGGATCAACTATTTGTTCCAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000730/539284‑539419 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttcaacaCTTTGTTGAACTTTAGCTTTTGTTTTTTTCCATTCACTGCCACCGAGTTTATTTAATTTTGGCGTCTTATCTTCCGAAGCTACATATTTTTGAACTTGATCCATTTGATCTACTGGAACAAATAGTTGATCCGTACCTTT  <  CP000730/539422‑539283                                                                                                                                                                                                                                                                                      AAGGTACGGATCAACTATTTGTTCCAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACACTTTG                                                                                                                                         <  1:270752/141‑1                         CAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACACTTTG                                                                                                                                         <  1:159778/117‑1                         CAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACACTTTG                                                                                                                                         >  2:159778/1‑117                                          CAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACACTTTGTTGAACTTTAGCTTTTGTTTTTTTCCATTCACTGCCACCGA                                                                                                >  2:363495/1‑141                                                     ATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACACTTTGTTGAACTTTAGCTTTTGTTTTTTTCCATTCACTGCCACCGAGTTTATTTAAT                                                                                     <  1:363495/141‑1                                                                                                                                  TTCAACACTTTGTTGAACTTTAGCTTTTGTTTTTTTCCATTCACTGCCACCGAGTTTATTTAATTTTGGCGTCTTATCTTCCGAAGCTACATATTTTTGAACTTGATCCATTTGATCTACTGGAACAAATAGTTGATCCGT        >  1:265933/1‑141                                                                                                                                        ACTTTGTTGAACTTTAGCTTTTGTTTTTTTCCATTCACTGCCACCGAGTTTATTTAATTTTGGCGTCTTATCTTCCGAAGCTACATATTTTTGAACTTGATCCATTTGATCTACTGGAACAAATAGTTGATCCGTACCTTT  >  1:61488/1‑141                                                                                                                                                                                                                                                                                      AAGGTACGGATCAACTATTTGTTCCAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000730/539284‑539419 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttcaacaCTTTGTTGAACTTTAGCTTTTGTTTTTTTCCATTCACTGCCACCGAGTTTATTTAATTTTGGCGTCTTATCTTCCGAAGCTACATATTTTTGAACTTGATCCATTTGATCTACTGGAACAAATAGTTGATCCGTACCTTT  <  CP000730/539422‑539283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.