New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 539419	35 (1.020)	5 (0.150)	4/262	4.9	13.1%	coding (1730/3507 nt)	mfd	transcription‑repair coupling factor helicase
	?	CP000730	= 539422	33 (1.020)					coding (1733/3507 nt)	mfd	transcription‑repair coupling factor helicase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAAGGTACGGATCAACTATTTGTTCCAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGAAGACACAGCTGAGCAAACAACATTTGAATTAGATTTTCCATATGAACTTACGCCTGACCAAGC  >  CP000730/539283‑539560                                                                                                                                            |                                                                                                                                           aaaGGTACGGATCAACTATTTGTTCCAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAgt                                                                                                                                           <  1:61488/141‑1 (MQ=255)       aCGGATCAACTATTTGTTCCAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGaa                                                                                                                                     <  1:265933/141‑1 (MQ=255)          gATCAACTATTTGTTCCAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGat                                                                                                                                  >  1:326605/1‑141 (MQ=255)           aTCAACTATTTGTTCCAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGata                                                                                                                                 >  1:149619/1‑141 (MQ=255)            tCAACTATTTGTTCCAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGatat                                                                                                                                >  1:67562/1‑141 (MQ=255)                     tGTTCCAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAaga                                                                                                                       >  1:78869/1‑141 (MQ=255)                        tCCAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTAGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGACAAAAACAAAAGCTAAAGTTCAACAAAGTGTTAAAGATATTgaagacgagat                                                                                                                    >  2:69602/1‑131 (MQ=255)                        tCCAGTAGATCAAATGGATCAAGGTCAAAACTATGTAGCTTCGGAAGATAAGCCGCCAAAATTAAATAAAATCGGCGGCAGGGAATGGAAAAAGACAAAAGCAACAGTTCAACAAAGAGTCGAAGAACTTGCTGAAGAGtc                                                                                                                    >  2:238125/1‑140 (MQ=255)                                aTCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTgattgatt                                                                                                            >  2:246751/1‑141 (MQ=255)                                   aaaTGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTtat                                                                                                         >  2:59076/1‑141 (MQ=255)                                   aaaTGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAATTGATTGATTtat                                                                                                         >  2:262039/1‑141 (MQ=255)                                     aTGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTtatat                                                                                                       >  2:180326/1‑141 (MQ=255)                                          tCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATaaaga                                                                                                  >  2:236375/1‑141 (MQ=255)                                          tCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATaaaga                                                                                                  <  1:349421/141‑1 (MQ=255)                                             aGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATaaagaaag                                                                                               >  1:72224/1‑141 (MQ=255)                                              gTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAaagaaaga                                                                                              >  1:1604/1‑141 (MQ=255)                                                        atGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAg                                                                                    >  1:189675/1‑141 (MQ=255)                                                           tAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAg                                                                                 >  1:296637/1‑141 (MQ=255)                                                                        tAAGCCGCCAAAATTAAATAAACTCGGTGGTAGTGAATGGGAACAAAGAAAAGCAAAAGTTCAACAAAGTGTTGAAGATATTGCTGCAGAGATGATTGAATTAAAGAAAGAAAGAGAAATGGCAGAAGGGTATCACTATgg                                                                    >  2:31788/1‑141 (MQ=255)                                                                          aGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATgggg                                                                  >  2:18176/1‑141 (MQ=255)                                                                            aCGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGaa                                                                >  2:206345/1‑141 (MQ=255)                                                                             cGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGAAg                                                               >  1:51094/1‑141 (MQ=255)                                                                                       aaataaaCTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTAGATAAAGAAAGAGAAATGGCAGAAGGGTATCAATATGGGGAAGACACAGCTGa                                                     >  1:164559/1‑141 (MQ=255)                                                                                           aaaCTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGAAGACACAGCTGAGCaa                                                 >  2:136269/1‑141 (MQ=255)                                                                                           aaaCTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGAAGACACAGCTGAGCaa                                                 <  2:135893/141‑1 (MQ=255)                                                                                                 ggTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTTCGGTAGAGTTTATTTAATAATATAAAGAAAGAGGAAGGGCGGAGGTGTATGAATATGGGGAAGACACAGGTTGGCAAacaaca                                           >  1:371511/1‑141 (MQ=255)                                                                                                      taGTAAACGGAAAAAAACAAAAGCTAAAGTAGAACAAAGTGAAGAAGATATTGATGAAGAGTTAATTGAGTTATACAAAGAAAGAGAGATGGCAGAAGGTTATCAAGATGGGAAATACACAGCTCAGCCAACAACAGTTGa                                      <  2:160891/140‑1 (MQ=255)                                                                                                          gAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGAAGACACAGCTGAGCAAACAACATTTGAATTa                                  <  1:105592/141‑1 (MQ=255)                                                                                                            aTGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTAGATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGAAGACACAGCTGAGCAAACAACATTTGAATTAGa                                >  1:72151/1‑141 (MQ=255)                                                                                                                aaaaaaaCAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGAAGACACAGCTGAGCAAACAACATTTGAATTAGAtttt                            <  1:234600/141‑1 (MQ=255)                                                                                                                       cAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGAAGACACAGCTGAGCAAACAACATTTGAATTAGATTTTCCATATg                     <  2:149619/141‑1 (MQ=255)                                                                                                                          aaGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGAAGACACAGCTGAGCAAACAACATTTGAATTAGATTTTCCATATGAAc                  <  2:114305/141‑1 (MQ=255)                                                                                                                                 aGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGAAGACACAGCTGAGCAAACAACATTTGAATTAGATTTTCCATATGAACTTACGcc           <  2:51094/141‑1 (MQ=255)                                                                                                                                         aaaGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGAAGACACAGCTGAGCAAACAACATTTGAATTAGATTTTCCATATGAACTTACGCCTGACCAAg   <  2:159367/141‑1 (MQ=255)                                                                                                                                          aaGGGTTGAAGATATTGGTGAAGAGTTGATTAATTTATATAAAGAAAGAGAAATGGAAGAGAGTTAGCAATATGGGGAAGACACAGATGAGCAAACAACATTTGAATTAGATTTTCCATATGAACTTACGCCTGACCAAGc  <  2:334928/141‑1 (MQ=255)                                                                                                                                            |                                                                                                                                           AAAGGTACGGATCAACTATTTGTTCCAGTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTAAATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAAGATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAATATGGGGAAGACACAGCTGAGCAAACAACATTTGAATTAGATTTTCCATATGAACTTACGCCTGACCAAGC  >  CP000730/539283‑539560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.