Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP000730	1,333,784	30 bp→TCGGTGGTCGCCGTATCATTAAAAAAAAAA	12.9%	coding (185‑214/1239 nt)	hflX →	GTP‑binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 1333783	70 (0.710)	8 (0.110) +TCGGTGGTCGCCGTATCATTAAAAAAAAAA	5/198	NT	12.9%	coding (184/1239 nt)	hflX	GTP‑binding protein
	?	CP000730	1333814 =	71 (0.720)					coding (215/1239 nt)	hflX	GTP‑binding protein

GCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000730/1333680‑1333783 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAAT  >  CP000730/1333814‑1333888                                                                                                         ||||||||||||||||||||||||||||||                                                                            GCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGAGATCGCCGTATCATTAAAAAAAAAAAAGAAAT                                                                      >  2:425792/1‑141                                             CAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTCGCCGTATCATAAAAAAAAAAGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAA                          >  2:890742/1‑141                                                     TGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTTGCCGTAGCAATAAAAAAAAAGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAA                  >  2:177770/1‑141                                                     TGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGCTGAA                  >  2:932236/1‑141                                                     TGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCGGTGGTCGCGGTAGCAAAAAAAAAAAAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAA                  >  2:426761/1‑141                                                         aacacttcaaGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAAATTCAAGCACTCATTGAGTTCAAAGATAATGATGTAGTAATCACAAATGATGAATTAA              >  2:801795/11‑141                                                             TGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAAATTCAAGCATTTATTGAGATCAAAGATATTGATGTAGACATCGCAAATGATGAATAAACGAa          >  2:104697/1‑140                                                                     TGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAAT  >  2:130305/1‑141                                                                                                         ||||||||||||||||||||||||||||||                                                                            GCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000730/1333680‑1333783 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAAT  >  CP000730/1333814‑1333888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.