New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 2538221 =40 (1.190)4 (0.130) 3/262 5.1 9.6% coding (266/687 nt) gpmA2 phosphoglycerate mutase
?CP000730 2538241 = 37 (1.160)coding (246/687 nt) gpmA2 phosphoglycerate mutase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAAAAGTAAGAGAAAATAACATTGCCATCGATGTAGCTTTTACATCGTTATTAACACGTGCTTTAGATACAACGCATTATATTTTAACTGAATCTAAACAACAATGGATTCCTGTATATAAAAGCTGGCGTTTAAATGAACGCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/2538365‑2538221
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aacgccaGCTTTTATATACAGGAATCCATTGTTGTTTAGATTCAGTTAAAATATAATGCGTTGTATCTAAAGCACGTGTTAATAACGATGTAAAAGCTACATCGATGGCAATGTTATTTTCTCTTACTTTTTCACCTGCTCT  >  CP000730/2538241‑2538375
                                                                                                                                                                                                                                                                                        
AAAAAGTAAGAGAAAATAACATTGCCATCGATGTAGCTTTTACATCGTTATTAACACGTGCTTTAGATACAACGCATTATATTTTAACTGAATCTAAACAACAATGGATTCCTGTATATAAAAGCTGGCGTTTAAATGAAC                                                                                                                                             <  1:119741/141‑1
AAAAAGTAAGAGAAAATAACATTGCCATCGATGTAGCTTTTACATCGTTATTAACACGTGCTTTAGATACAACGCATTATATTTTAACTGAATCTAAACAACAATGGATTCCTGTATATAAAAGCTGGCGTTTAAATGAAC                                                                                                                                             <  1:151362/141‑1
    AGTAAGAGAAAATAACATTGCCATCGATGTAGCTTTTACATCGTTATTAACACGTGCTTTAGATACAACGCATTATATTTTAACTGAATCTAAACAACAATGGATTCCTGTATATAAAAGCTGGCGTTTAAATGAACGCCA                                                                                                                                         >  2:91959/1‑141
                                                     ACACGTGCTTTAGATACAACGCATTATATTTTAACTGAATCTAAACAACAATGGATTCCTGTATATAAAAGCTGGCGTTTAAATGAACGCCAGCTTTTATATACAGGAATCCATTGTTGTTTAGATTCAGTTAAAATATAA                                                                                        >  1:134175/1‑141
                                                                                                AACAACAATGGATTCCTGTATATAAAAGCTGGCGTTTAAATGAACGCCAGCTTTTATATACAGGAATCCATTGTTGTTTAGATTCAGTTAAAATATAATGCGTTGTATCTAAAGCACGTGTTAATAACGATGTAAAAGCTA                                             <  2:134175/141‑1
                                                                                                                                    TAAATGAACGCCAGCTTTTATATACAGGAATCCATTGTTGTTTAGATTCAGTTAAAATATAATGCGTTGTATCTAAAGCACGTGTTAATAACGATGTAAAAGCTACATCGATGGCAATGTTATTTTCTCTTACTTTTTCAC         >  1:112751/1‑141
                                                                                                                                    TAAATGAACGCCAGCTTTTATATACAGGAATCCATTGTTGTTTAGATTCAGTTAAAATATAATGCGTTGTATCTAAAGCACGTGTTAATAACGATGTAAAAGCTACATCGATGGCAATGTTATTTTCTCTTACTTTTGCAC         >  1:22126/1‑141
                                                                                                                                          AACGCCAGCTTTTATATACAGGAATCCATTGTTGTTTAGATTCAGTTAAAATATAATGCGTTGTATCTAAAGCACGTGTTAATAACGATGTAAAAGCTACATCGATGGCAATGTTATTTTCTCTTACTTTTTCACCTGCTC   >  2:142346/1‑141
                                                                                                                                           ACGCCAGCTTTTATATACAGGAATCCATTGTTGTTTATATTCAGTTAAAATATAATGCGTTGTATCTAAAGCACGTGTTAGTAACGATGTAAAAGCTACATCGATGGCAATGTTATTTTCTCTTACTTTTTCACCTGCTtt  <  2:324861/141‑3
                                                                                                                                                                                                                                                                                        
AAAAAGTAAGAGAAAATAACATTGCCATCGATGTAGCTTTTACATCGTTATTAACACGTGCTTTAGATACAACGCATTATATTTTAACTGAATCTAAACAACAATGGATTCCTGTATATAAAAGCTGGCGTTTAAATGAACGCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/2538365‑2538221
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aacgccaGCTTTTATATACAGGAATCCATTGTTGTTTAGATTCAGTTAAAATATAATGCGTTGTATCTAAAGCACGTGTTAATAACGATGTAAAAGCTACATCGATGGCAATGTTATTTTCTCTTACTTTTTCACCTGCTCT  >  CP000730/2538241‑2538375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.