New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 562451 =34 (1.010)4 (0.130) 3/250 4.9 10.6% intergenic (+386/+138) rrf3/USA300HOU_0510 5S ribosomal RNA/GntR family transcriptional regulator
?CP000730 562497 = 37 (1.220)intergenic (+432/+92) rrf3/USA300HOU_0510 5S ribosomal RNA/GntR family transcriptional regulator
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGATTGTCTAAGTATGTACAACTTTAATTTTGTGTTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTATAATTAATGATTTTATTAGAGCGTCTACATGCGGT  >  CP000730/562364‑562631
                                                                                                                                     |                                                                                                                                      
cGATTGTCTAAGTATGTACAACTTTAATTTTGTGTTTATATAAATTTAAAATGATATCATCGAGGTCAAAATATTGGATAAATAGATGAGTGCAGGAAGACTGGAGCTAATTGAAAATGATCTTACTGCTCTTTTATGTAc                                                                                                                                 >  1:134417/1‑141 (MQ=255)
cGATTGTCTAAGTATGTACAACTTTAATTTTGTGTTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATATTACTGCTCTTTTATATAc                                                                                                                                 <  2:58076/141‑1 (MQ=255)
 gATTGTCTAAGTATGTACAACTTTAATTTTGTGTTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACt                                                                                                                                <  1:239714/141‑1 (MQ=255)
         aaGTATGTACAACTTTAATTTTGTGTTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGaa                                                                                                                        <  2:120328/141‑1 (MQ=255)
           gTATGTACAACTTTAATTTTGTGTTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAAt                                                                                                                      <  1:223506/141‑1 (MQ=255)
             aTGTACAACTTTAATTTTGTGTTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATAc                                                                                                                    <  1:1481/141‑1 (MQ=255)
                     cTTTAATTTTGTGTTTATATCAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGCAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGa                                                                                                            >  1:81142/1‑141 (MQ=255)
                         aaTTTTGTGTTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGTAaatt                                                                                                        >  2:242083/1‑141 (MQ=255)
                         aaTTTTGTGTTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAaatt                                                                                                        >  1:14684/1‑141 (MQ=255)
                         aaTTTTGTGTTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAaatt                                                                                                        >  2:387826/1‑141 (MQ=255)
                            tttGTGTTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAaattaat                                                                                                     >  2:210230/1‑141 (MQ=255)
                              tgtgTTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAattaatta                                                                                                   <  1:355779/141‑1 (MQ=255)
                               gtgtTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAattat                                                                                                  >  2:272022/1‑141 (MQ=255)
                               gtggctatataAATTTAAATTGATATCATCGAACACTAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTCATTGAAAATGATCTTACTGCTCTTTTAGATACTTTATTGAAATACAAAAAGGACATTAagtat                                                                                                  >  2:76371/6‑141 (MQ=255)
                                  tttATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATtattat                                                                                               <  1:262809/141‑1 (MQ=255)
                                  attATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATtattat                                                                                               <  2:134417/140‑1 (MQ=255)
                                      tataAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACaa                                                                                           >  2:58235/1‑141 (MQ=255)
                                          aaTTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGa                                                                                       >  1:98855/1‑141 (MQ=255)
                                              tAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATATAAATAAAAATGGTCTTACTGCACTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAg                                                                                   >  1:332971/1‑141 (MQ=39)
                                                 aaTGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTa                                                                                >  1:40322/1‑141 (MQ=255)
                                                  aTGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTAt                                                                               >  1:160985/1‑141 (MQ=255)
                                                                aaCAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAacac                                                                 >  2:244220/1‑141 (MQ=255)
                                                                 aCAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACt                                                                <  1:212667/141‑1 (MQ=255)
                                                                            tataAAGAGATAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCa                                                     <  1:26087/141‑1 (MQ=255)
                                                                               aaaTAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAaag                                                  <  2:121044/141‑1 (MQ=255)
                                                                                aaTAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAaaga                                                 <  1:244764/141‑1 (MQ=255)
                                                                                          agCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTGATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATTAGTAACACTAACTTTTATCTAAGAAGTGTTACtt                                       <  2:314558/141‑1 (MQ=255)
                                                                                           gCAGTAAGACGGTATCTAATTGAAAACGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACttt                                      <  2:301606/141‑1 (MQ=255)
                                                                                                acgACGGAATCTAATTGAAAATGCGCCTACTGCTCTTTTATATACTTTGTTGAAATACGAAGAGGAACTTAATGATTATACAATTGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAATTGTTACTTTAtaat                                 >  2:221719/3‑141 (MQ=21)
                                                                                                   aCGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTAtaattaa                              >  1:285650/1‑141 (MQ=255)
                                                                                                    cGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTAtaattaat                             <  1:269358/141‑1 (MQ=255)
                                                                                                    aggTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATAGAAAAAAAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTAtaattaat                             <  2:155327/140‑1 (MQ=255)
                                                                                                     ggTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTATAATTAATg                            <  1:81997/141‑1 (MQ=255)
                                                                                                        aTCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTATAATTAATGAtt                         >  2:362268/1‑141 (MQ=255)
                                                                                                           tAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTATAATTAATGATTtta                      >  1:204233/1‑141 (MQ=255)
                                                                                                             aTTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTATAATTAATGATTttatt                    <  1:272022/141‑1 (MQ=255)
                                                                                                                     tGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTATAATTAATGATTTTATTAGAGCGTc            <  2:160985/141‑1 (MQ=255)
                                                                                                                            tcTGCTCTTTTTTATACTTTTTTCAATTACGAAAAGCATATTAATTATGATACTATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTATAATTAATGATTTTATTAGAGCGTCTACATGc     <  1:275691/140‑1 (MQ=21)
                                                                                                                            aCTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTATAATTAATGATTTTATTAGAGCGTCTACATGc     <  1:221262/141‑1 (MQ=255)
                                                                                                                               gCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTATAATTAATGATTTTATTAGAGCGTCTACATGCGGt  <  1:378647/141‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
CGATTGTCTAAGTATGTACAACTTTAATTTTGTGTTTATATAAATTTAAAATGATATCATCGAAAACAAAATATTGTATAAATAGAGAAGAGCAGTAAGACGGTATCTAATTGAAAATGATCTTACTGCTCTTTTATATACTTTATTGAAATACAAAAAGGAAATTAATTATTATACAATAGACAAGCTATTGCATAAGTAACACTAACTTTTATCAAAGAAGTGTTACTTTATAATTAATGATTTTATTAGAGCGTCTACATGCGGT  >  CP000730/562364‑562631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.