New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	1236098 =	70 (1.010)	3 (0.050)	3/282	2.2	4.9%	coding (254/642 nt)	yloS	thiamine diphosphokinase
	?	CP000730	1236143 =	50 (0.770)					coding (299/642 nt)	yloS	thiamine diphosphokinase
Rejected: Frequency below/above cutoff threshold.

TTCAGTCAGTAAAGAAGAACGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGCCTAAAGGTCAACATACAGTTGAAAAAGATAAG  >  CP000730/1235994‑1236288                                                                                                                                                      |                                                                                                                                                   aattttattttgttgatcgataacttctatatgaacatcatgtttataatatgcttttttcaataataactgaattgccccaaaaaagtgatctaatcgtccgcCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGc                                                                                                                                                   >  1:788395‑M1/105‑151 (MQ=255)   cagtcaatAAATAAAAACGCCAACTTCTAACAGAACAGTTATAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTATAATTTATGGTGCAACAGGCgg                                                                                                                                                 <  2:667675/151‑1 (MQ=255)    agtcagtAAAGAAGAACGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGa                                                                                                                                                <  1:264773/151‑1 (MQ=255)      tattttgttgatcgataacttctatatgaacatcatgtttataatatgcttttttcaataataactgaattgccccaaaaaagtgatctaatcgtccgcCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACg                                                                                                                                              >  2:434594‑M1/100‑151 (MQ=255)         gtAAAGAAGAACGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGAtt                                                                                                                                           >  1:1048302/1‑151 (MQ=255)          tAAAGAAGAACGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACACTTTATGGTGCAACAGGCGGACGATTa                                                                                                                                          >  2:70703/1‑151 (MQ=255)          tAAAGAAGAACGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTa                                                                                                                                          <  2:648461/151‑1 (MQ=255)           aaaGAAGAACGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAg                                                                                                                                         >  2:336298/1‑151 (MQ=255)           aaaGAAGAACGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAg                                                                                                                                         >  1:599910/1‑151 (MQ=255)            aagaagAACGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGa                                                                                                                                        >  2:910434/1‑151 (MQ=255)             agaagaACGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGAt                                                                                                                                       <  1:15653/151‑1 (MQ=255)                agagCGCTAACTTCTAACAGATCATTTACAAATCAAACCAGTTCTGGCTGAATAAGCTGATACGGATTTAGCTTTAGTGGTTGATAAAGCTGTTGCACTTGGATGTGATAGTATTACGATTTATGGTGCAACAGGCGGACGATTAGATCAc                                                                                                                                    <  2:277811/151‑1 (MQ=255)                agaaCGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGGCGATTAGCTCAc                                                                                                                                    >  1:459222/1‑151 (MQ=255)                agaaCGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCAc                                                                                                                                    >  1:927463/1‑151 (MQ=255)                 gaaCGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACt                                                                                                                                   >  2:486126/1‑151 (MQ=255)                          cTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTgggg                                                                                                                          >  1:557281/1‑151 (MQ=255)                           ttCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGc                                                                                                                         <  1:413329/151‑1 (MQ=255)                           ttCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGc                                                                                                                         >  1:570679/1‑151 (MQ=255)                            tCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCa                                                                                                                        >  2:727491/1‑151 (MQ=255)                               aacagaacagTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAAtt                                                                                                                     >  1:804422/1‑151 (MQ=255)                                acagaacagTTACAAATCAAACCAGTTCAAGCTGTAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTc                                                                                                                    <  1:981467/151‑1 (MQ=255)                                   gaacagTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGt                                                                                                                 <  1:1023730/151‑1 (MQ=255)                                          tACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTattatt                                                                                                          <  1:957575/151‑1 (MQ=255)                                           aCAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTg                                                                                                         <  1:954512/151‑1 (MQ=255)                                             aaaTCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑aa                                                                                                      <  2:585740/151‑1 (MQ=255)                                                  aaaCCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAg                                                                                                 <  1:578834/151‑1 (MQ=255)                                                  aaaCCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAg                                                                                                 >  1:608461/1‑151 (MQ=255)                                                    aCCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCa                                                                                               <  2:664652/151‑1 (MQ=255)                                                           cAAGATGAAAAAGCGAATACGGATTTAACTTAAGCAGATAATAAAAATGTTGAAATTGGATTTGAGAGTAGTACAATTTATGGTGCAACAGGCAGACGATTAGATCATTTTTTTGTGGCAATTCAAAAAAAAAAA‑AAAAAAGCATATtata                                                                                        <  2:10864/151‑1 (MQ=255)                                                            aaGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATaa                                                                                       <  1:48123/151‑1 (MQ=255)                                                                tGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACat                                                                                   >  1:556557/1‑151 (MQ=255)                                                                       gCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTc                                                                            >  1:355409/1‑151 (MQ=255)                                                                         tGATACGGATTTAGCTTTAGCGGTTGATACAGCTCTCTTCCAGCGTTTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCat                                                                          >  1:515429/1‑151 (MQ=255)                                                                           aTACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCatat                                                                        <  1:902236/151‑1 (MQ=255)                                                                                gATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCGTATTATATTCATGATGTTCATATAGAAg                                                                   <  2:508335/151‑1 (MQ=255)                                                                                 aTTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGt                                                                  >  1:884875/1‑151 (MQ=255)                                                                                   ttagctttagcGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTa                                                                <  2:1048302/151‑1 (MQ=255)                                                                                        tttagcGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGAt                                                           <  1:1012435/151‑1 (MQ=255)                                                                                         ttagcGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATGAACATCATGTTtataatatgcttttttcaa                                                          <  1:941384/151‑16 (MQ=255)                                                                                         ttagcGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATc                                                          <  1:741599/151‑1 (MQ=255)                                                                                          tagcGGTTGATAAAGCTGTTGCACTTGGTTTTGATATTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTAAAGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATca                                                         <  2:967151/151‑1 (MQ=255)                                                                                             cGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATcaaca                                                      >  2:625190/1‑151 (MQ=255)                                                                                                 tgctAAAGCTGTTCAACCTGGTTTGGTTAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACaaaat                                                  <  1:152376/148‑1 (MQ=255)                                                                                                  cgtccgcctgttgcaccataaattgtaatactatcaaatccaagtgcaacagGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACaaaata                                                 >  2:434594‑M2/53‑151 (MQ=255)                                                                                                   aTAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACaaaataa                                                <  1:899972/151‑1 (MQ=255)                                                                                                       gcctgttgcaccataaattgtaatactatcaaatccaagtgcaacagGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAAtt                                            >  1:788395‑M2/48‑151 (MQ=255)                                                                                                          TGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGaa                                         <  2:653537‑M1/151‑1 (MQ=255)                                                                                                                ACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTAATAAAAA‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTg                                   >  2:58427‑M1/1‑151 (MQ=255)                                                                                                                  ttGGATTTGATAGTATTGTGATTAATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGcc                                 <  1:9268/151‑1 (MQ=255)                                                                                                                  ttGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGcc                                 <  2:339913/151‑1 (MQ=255)                                                                                                                           aTAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGCCTAAAGGTCa                        <  1:408761/151‑1 (MQ=255)                                                                                                                             aGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTAAAA‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGCCTAAAGGTCAAc                      <  2:99743/151‑1 (MQ=255)                                                                                                                                 ttACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTGAAAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGCCTAAAGGTCAACATa                   >  1:547846/1‑151 (MQ=255)                                                                                                                                      aatccaagtgcaacagGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGCCTAAAGGTCAACATACAGTTg             <  1:434594‑M2/135‑1 (MQ=255)                                                                                                                                       tttATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGCCTAAAGGTCAACATACAGTTGa            <  1:361538/151‑1 (MQ=255)                                                                                                                                            ggTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGCCTAAAGGTCAACATACAGTTGAAAAAg       >  1:466250/1‑151 (MQ=255)                                                                                                                                            ggTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGCATAAAGGTCAACATACAGTTGAATAAg       >  2:77044/1‑151 (MQ=255)                                                                                                                                                 aacagGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGCCTAAAGGTCAACATACAGTTGAAAAAGATAag  <  1:21767‑M2/146‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                   TTCAGTCAGTAAAGAAGAACGCCAACTTCTAACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTAGCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTG‑AAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGCCTAAAGGTCAACATACAGTTGAAAAAGATAAG  >  CP000730/1235994‑1236288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.