mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.26.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | 7Glu_S9_L001_R1_001 | 988,506 | 281,121,939 | 100.0% | 284.4 bases | 301 bases | 96.9% |
| errors | 7Glu_S9_L001_R2_001 | 988,477 | 282,700,773 | 100.0% | 286.0 bases | 301 bases | 80.8% |
| total | 1,976,983 | 563,822,712 | 100.0% | 285.2 bases | 301 bases | 88.9% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 112.2 | 3.8 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 1903 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 107 |
| Total length of all junction candidates (factor times the reference genome length) | ≥ 0.1 | 0.014 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.89360 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum coverage each strand | 2 |
| Polymorphism bias cutoff | 0.001 |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥2 bases |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 18:27:36 15 Apr 2015 | 18:28:37 15 Apr 2015 | 1 minute 1 second |
| Read alignment to reference genome | 18:28:37 15 Apr 2015 | 18:35:08 15 Apr 2015 | 6 minutes 31 seconds |
| Preprocessing alignments for candidate junction identification | 18:35:08 15 Apr 2015 | 18:36:16 15 Apr 2015 | 1 minute 8 seconds |
| Preliminary analysis of coverage distribution | 18:36:16 15 Apr 2015 | 18:39:16 15 Apr 2015 | 3 minutes 0 seconds |
| Identifying junction candidates | 18:39:16 15 Apr 2015 | 18:39:17 15 Apr 2015 | 1 second |
| Re-alignment to junction candidates | 18:39:17 15 Apr 2015 | 18:39:46 15 Apr 2015 | 29 seconds |
| Resolving alignments with junction candidates | 18:39:46 15 Apr 2015 | 18:41:36 15 Apr 2015 | 1 minute 50 seconds |
| Creating BAM files | 18:41:36 15 Apr 2015 | 18:43:44 15 Apr 2015 | 2 minutes 8 seconds |
| Tabulating error counts | 18:43:44 15 Apr 2015 | 18:46:36 15 Apr 2015 | 2 minutes 52 seconds |
| Re-calibrating base error rates | 18:46:36 15 Apr 2015 | 18:46:37 15 Apr 2015 | 1 second |
| Examining read alignment evidence | 18:46:37 15 Apr 2015 | 21:34:53 15 Apr 2015 | 2 hours 48 minutes 16 seconds |
| Polymorphism statistics | 21:34:53 15 Apr 2015 | 21:34:54 15 Apr 2015 | 1 second |
| Output | 21:34:54 15 Apr 2015 | 21:36:06 15 Apr 2015 | 1 minute 12 seconds |
| Total | 3 hours 8 minutes 30 seconds | ||
