breseq  version 0.26.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 526,792 A→C 20.2% S1178R (AGC→CGC)  rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 526,800 A→G 15.9% E1180E (GAA→GAG rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 1,311,197 G→T 17.9% E37* (GAA→TAA)  tonB → membrane spanning protein in TonB‑ExbB‑ExbD transport complex
RA 1,466,711 T→C 7.2% pseudogene (1320/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,529,175 T→C 100% pseudogene (1274/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,180 T→C 100% pseudogene (1279/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,185 C→T 100% pseudogene (1284/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,203 C→A 100% pseudogene (1302/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,218 C→T 100% pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,679,869 A→T 6.6% I105F (ATC→TTC)  ydgI → putative arginine/ornithine antiporter transporter
RA 1,708,765 T→A 8.4% V616D (GTC→GAC)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,756,457 +A 80.0% coding (760/1413 nt) pykF → pyruvate kinase I
RA 1,796,524 A→G 6.0% I474T (ATC→ACC)  pheT ← phenylalanine tRNA synthetase, beta subunit
JC JC 1,907,562 IS1 (+) +9 bp 100% coding (22‑30/144 nt) yobF ← DUF2527 family heat‑induced protein
RA 1,915,288 G→C 10.4% L83V (CTT→GTT)  proQ ← RNA chaperone, putative ProP translation regulator
RA 2,173,359 Δ1 bp 7.4% pseudogene (4/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,173,360 Δ1 bp 7.4% pseudogene (3/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,966,582 C→T 80.4% G618D (GGC→GAC)  ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
RA 3,440,065 G→A 12.6% P322L (CCA→CTA)  rpoA ← RNA polymerase, alpha subunit
RA 3,560,455 +G 93.8% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 4,296,190 A→G 24.7% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 33.4% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 23.7% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,286 C→T 17.2% intergenic (+492/+150) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,304 A→C 9.8% intergenic (+510/+132) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380 +C 92.7% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380 +G 92.7% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1211045 1212812 1768 47 [45] [45] 46 [mcrA]–[ycgX] [mcrA], icdC, iraM, [ycgX]
* * ÷ NC_000913 1978373 1979728 1356 47 [41] [45] 46 insB1–insA insB1, insA
* * ÷ NC_000913 2469052 2470736 1685 47 [44] [43] 48 [gtrB]–gtrS [gtrB], gtrS
* * ÷ NC_000913 3267939 3269705 1767 46 [44] [45] 46 [yhaB]–yhaC [yhaB], yhaC
* * ÷ NC_000913 4002599 4003407 809 48 [44] [45] 46 [yigF]–[rarD] [yigF], yigG, [rarD]
* * ÷ NC_000913 4476521 4477228 708 50 [34] [45] 47 yjgL SopA‑central‑domain‑like hexapeptide repeat protein

Unassigned new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =55 (0.540)30 (0.310) 26/516 NT 39.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 41 (0.430)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780548 (0.470)28 (0.290) 24/516 NT 39.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 41 (0.430)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)24 (0.240) 19/540 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19785020 (0.000)23 (0.230) 17/532 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 3194098 =100 (0.980)3 (0.030) 3/536 NT 3.0% coding (1235/1662 nt) yqiK PHB family membrane protein, function unknown
?NC_000913 3194119 = 98 (0.980)coding (1256/1662 nt) yqiK PHB family membrane protein, function unknown