breseq version 0.29.0 revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 202 shown) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 357,880 | 0 | T | C | 60.0% | 5.6 / 23.6 | 25 | K271E (AAA→GAA) | cynR | transcriptional activator of cyn operon; autorepressor |
* | NC_000913 | 357,890 | 0 | C | A | 56.5% | ‑4.4 / 17.9 | 23 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
* | NC_000913 | 357,875 | 0 | A | T | 56.0% | 7.8 / 30.6 | 26 | N272K (AAT→AAA) | cynR | transcriptional activator of cyn operon; autorepressor |
* | NC_000913 | 357,887 | 0 | C | A | 54.5% | ‑2.0 / 19.4 | 22 | L268F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
* | NC_000913 | 354,288 | 0 | T | G | 50.9% | 54.0 / 83.8 | 53 | V528G (GTG→GGG) | prpE | propionate‑‑CoA ligase |
* | NC_000913 | 2,434,882 | 0 | T | G | 48.9% | 62.6 / 14.3 | 48 | Y252S (TAC→TCC) | truA | tRNA pseudouridine(38‑40) synthase |
* | NC_000913 | 2,421,443 | 0 | A | C | 47.9% | 63.6 / 17.0 | 48 | Y40S (TAC→TCC) | folX | D‑erythro‑7,8‑dihydroneopterin triphosphate 2'‑epimerase and dihydroneopterin aldolase |
* | NC_000913 | 29,496 | 0 | T | A | 47.7% | 34.8 / 26.9 | 44 | intergenic (+301/‑155) | dapB/carA | dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
* | NC_000913 | 29,486 | 0 | C | A | 47.4% | 32.7 / 28.7 | 38 | intergenic (+291/‑165) | dapB/carA | dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
* | NC_000913 | 1,413,735 | 0 | A | T | 46.5% | 33.5 / 36.5 | 43 | Y2* (TAT→TAA) | ydaC | DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein |
* | NC_000913 | 354,282 | 0 | T | G | 46.3% | 69.6 / 10.3 | 54 | V526G (GTT→GGT) | prpE | propionate‑‑CoA ligase |
* | NC_000913 | 3,443,277 | 0 | A | C | 45.0% | 46.3 / 23.9 | 40 | V274G (GTA→GGA) | secY | preprotein translocase membrane subunit |
* | NC_000913 | 2,029,274 | 0 | C | T | 44.9% | 36.1 / 25.3 | 49 | K31K (AAG→AAA) | yedI | DUF808 family inner membrane protein |
* | NC_000913 | 2,252,646 | 0 | T | G | 44.4% | 65.9 / 11.5 | 46 | V316G (GTA→GGA) | yeiI | putative kinase |
* | NC_000913 | 4,630,340 | 0 | A | C | 44.4% | 57.1 / 13.4 | 46 | G61G (GGT→GGG) | ettA | energy‑dependent translational throttle A |
* | NC_000913 | 4,429,984 | 0 | T | G | 43.4% | 73.0 / 12.0 | 53 | L41V (TTG→GTG) | cycA | D‑alanine/D‑serine/glycine transporter |
* | NC_000913 | 4,171,892 | 0 | C | G | 43.3% | 38.9 / 10.6 | 30 | intergenic (+136/‑165) | rrfB/murB | 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding |
* | NC_000913 | 1,604,237 | 0 | C | T | 43.1% | 64.1 / 24.7 | 59 | S64L (TCG→TTG) | lsrD | autoinducer 2 import system permease protein |
* | NC_000913 | 436,516 | 0 | C | A | 42.9% | 63.8 / 21.2 | 49 | Q295K (CAA→AAA) | thiL | thiamine monophosphate kinase |
* | NC_000913 | 941,713 | 0 | T | G | 42.2% | 66.3 / 12.5 | 45 | V252G (GTG→GGG) | dmsA | dimethyl sulfoxide reductase, anaerobic, subunit A |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1207805 | 48 (0.860) | 17 (0.340) | 15/266 | 3.9 | 28.8% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 41 (0.830) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related |