Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 657,215 Δ25 bp intergenic (+98/‑53) pagP → / → cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 657215 657239 25 55 [0] [0] 56 pagP/cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 6572140 (0.000)55 (0.910) 51/296 0.3 100% intergenic (+97/‑78) pagP/cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
?NC_000913 657240 = 0 (0.000)intergenic (+123/‑52) pagP/cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

ACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAG  >  NC_000913/657074‑657361
                                                                                                                                            |                                                                                                                                                   
acaaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAACTAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaacc                                                                                                                                           >  2:416420‑M1/1‑141 (MQ=255)
 caaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccg                                                                                                                                          <  2:330105‑M1/151‑12 (MQ=255)
        catGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagc                                                                                                                                   >  2:777227‑M1/2‑133 (MQ=255)
             gtACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcattt                                                                                                                               <  1:21928‑M1/150‑23 (MQ=255)
                    gCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAAGCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtcta                                                                                                                        <  2:242100‑M1/150‑30 (MQ=255)
                           tGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcat                                                                                                                >  1:271194‑M1/1‑114 (MQ=255)
                            gcgcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatg                                                                                                               <  1:395111‑M1/151‑39 (MQ=255)
                                tttCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaa                                                                                                            >  2:489200‑M1/1‑109 (MQ=255)
                                 ttCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaa                                                                                                            <  2:861527‑M1/149‑42 (MQ=255)
                                  tCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaagg                                                                                                         >  1:514611‑M1/1‑107 (MQ=255)
                                  tCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaag                                                                                                          <  1:411428‑M1/150‑44 (MQ=255)
                                     gTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaa                                                                                                      <  1:427482‑M1/151‑48 (MQ=255)
                                          gagaCAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttg                                                                                                 <  1:499913‑M1/151‑53 (MQ=255)
                                               aaaTGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACCTTTATCACTTTTTAGTAAAGTTACACTactggtaatcgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtc                                                                                            >  2:598665‑M1/1‑94 (MQ=255)
                                                 aTGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtcta                                                                                          <  2:540127‑M1/151‑60 (MQ=255)
                                                   gAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaag                                                                                        <  2:105603‑M1/151‑62 (MQ=255)
                                                     aGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtcta                                                                                          >  1:555956‑M1/1‑88 (MQ=255)
                                                       ttttAGTAACTTCTTTAAAATCAAGAGCGAAAATAAGTAACATCAAAAATAACGCGGCTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaatggtaattttgatgcctaagatt                                                                                     >  2:956491‑M1/1‑86 (MQ=255)
                                                        tttAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagatta                                                                                    <  1:728480‑M1/150‑66 (MQ=255)
                                                           aGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaagg                                                                                >  1:68816‑M1/1‑82 (MQ=255)
                                                            gTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggt                                                                               <  1:620974‑M1/151‑71 (MQ=255)
                                                                  tcttTAAAATCAATAGCTAAAATAATTAACATCAAAAATAACGCGACTTTTATCACTTTTTATTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacg                                                                           <  2:476072‑M1/149‑75 (MQ=255)
                                                                   cttTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgtt                                                                         >  1:672992‑M1/1‑74 (MQ=255)
                                                                     ttAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgtt                                                                         <  1:576291‑M1/148‑77 (MQ=255)
                                                                        aaaTCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtgg                                                                   >  2:225216‑M1/1‑69 (MQ=255)
                                                                            cAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggttta                                                               <  2:911144‑M1/151‑87 (MQ=255)
                                                                            cAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggttt                                                                <  1:59783‑M1/150‑86 (MQ=255)
                                                                             aaTAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggttta                                                               >  2:558319‑M1/1‑64 (MQ=255)
                                                                             aaTAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggttt                                                                >  2:251287‑M1/1‑64 (MQ=255)
                                                                                 gCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgag                                                          <  1:836406‑M1/151‑92 (MQ=255)
                                                                                     aaaTAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtcc                                                       >  1:605383‑M1/1‑56 (MQ=255)
                                                                                      aaTAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTATTAAAGTTTCACTactggtaaccgacacagcatttgtgtttatttttcatttaaaggtaattttgatgtctaagattaaaagtaacgttaggtggtttaatgagtcca                                                      <  2:963036‑M1/150‑96 (MQ=255)
                                                                                        tAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaa                                                     >  1:432478‑M1/1‑53 (MQ=255)
                                                                                             aaCATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattc                                              >  1:792936‑M1/1‑48 (MQ=255)
                                                                                                    aaaaTAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttc                                        >  1:303000‑M1/1‑41 (MQ=255)
                                                                                                    aaaaTAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggaaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttc                                        >  2:104218‑M1/1‑41 (MQ=255)
                                                                                                       aTAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcatt                                     >  2:474339‑M1/1‑38 (MQ=255)
                                                                                                        tAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattac                                   >  1:558976‑M1/1‑37 (MQ=255)
                                                                                                         aaCGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattact                                  >  2:198506‑M1/1‑36 (MQ=255)
                                                                                                          aCGCGACTTTTATCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactc                                 >  1:13556‑M1/1‑35 (MQ=255)
                                                                                                                     aTCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggc                      <  2:544704‑M1/151‑128 (MQ=255)
                                                                                                                     aTCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacgg                       <  1:563977‑M1/150‑127 (MQ=255)
                                                                                                                      tCACTTTTTAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggc                      <  1:233585‑M1/150‑128 (MQ=255)
                                                                                                                           ttttAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggcagcaa                 <  1:674050‑M1/150‑133 (MQ=255)
                                                                                                                           ttttAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggcagca                  <  2:108576‑M1/149‑132 (MQ=255)
                                                                                                                            tttAGTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggcagcaaa                <  1:142181‑M1/150‑134 (MQ=255)
                                                                                                                                gTAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggcagcaaagacgt           <  1:57957‑M1/151‑139 (MQ=255)
                                                                                                                                 tAAAGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggcagcaaagacgt           >  1:822826‑M1/1‑12 (MQ=255)
                                                                                                                                  aaaGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggcagcaaagacgtgt         <  2:320404‑M1/151‑141 (MQ=255)
                                                                                                                                   aaGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggcagcaaagacgtgtt        >  2:126034‑M1/1‑10 (MQ=255)
                                                                                                                                   aaGTTACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggcagcaaagacgtgtt        <  1:561293‑M1/151‑142 (MQ=255)
                                                                                                                                      ttACACTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggcagcaaagacgtgttcgt     >  2:3377‑M1/1‑7 (MQ=255)
                                                                                                                                       tACACTactggtaaccgactcagcatttgtgtctatttttcatgtaaaggtatttttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggcagcaaagacgtgttcgt     >  2:664137‑M1/1‑6 (MQ=255)
                                                                                                                                        acacTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggcagcaaagacgtgttcgtac   <  2:432282‑M1/151‑147 (MQ=255)
                                                                                                                                         cacTactggtaaccgacacagcatttgtgtctatttttcatgtaaaggtaattttgatgtctaagattaaaggtaacgttaagtggtttaatgagtccaaaggattcggtttcattactccggaagacggcagcaaagacgtgttcgtaca  <  1:672942‑M1/151‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
ACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAG  >  NC_000913/657074‑657361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.