Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 657,215 Δ25 bp intergenic (+98/‑53) pagP → / → cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 657215 657239 25 55 [0] [0] 56 pagP/cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 6572140 (0.000)55 (0.910) 51/296 0.3 100% intergenic (+97/‑78) pagP/cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
?NC_000913 657240 = 0 (0.000)intergenic (+123/‑52) pagP/cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

GATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTC  >  NC_000913/657099‑657390
                                                                                                                                             |                                                                                                                                                      
acaatggcaatgtgtactttgcctggatgcgctttcagttttgagacaaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaactaacgcgacttttatcactttttagtaaagttacactACTGGTAAcc                                                                                                                                               >  2:416420‑M2/142‑151 (MQ=255)
 caatggcaatgtgtactttgcctggatgcgctttcagttttgagacaaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCg                                                                                                                                              <  2:330105‑M2/11‑1 (MQ=255)
        catgtgtactttgcctggatgcgctttcagttttgagacaaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGc                                                                                                                                       >  2:777227‑M2/134‑151 (MQ=255)
             gtactttgcctggatgcgctttcagttttgagacaaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCAttt                                                                                                                                   <  1:21928‑M2/22‑1 (MQ=255)
                    gcctggatgcgctttcagttttgagacaaatgaagttttagtaacttctttaaaagcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTa                                                                                                                            <  2:242100‑M2/29‑1 (MQ=255)
                           tgcgctttcagttttgagacaaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCAt                                                                                                                    >  1:271194‑M2/115‑151 (MQ=255)
                            gcgctttcagttttgagacaaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATg                                                                                                                   <  1:395111‑M2/38‑1 (MQ=255)
                                tttcagttttgagacaaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTaa                                                                                                                >  2:489200‑M2/110‑150 (MQ=255)
                                 ttcagttttgagacaaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTaa                                                                                                                <  2:861527‑M2/41‑1 (MQ=255)
                                  tcagttttgagacaaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAgg                                                                                                             >  1:514611‑M2/108‑151 (MQ=255)
                                  tcagttttgagacaaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAg                                                                                                              <  1:411428‑M2/43‑1 (MQ=255)
                                     gttttgagacaaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTaa                                                                                                          <  1:427482‑M2/47‑1 (MQ=255)
                                          gagacaaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTg                                                                                                     <  1:499913‑M2/52‑1 (MQ=255)
                                               aaatgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacctttatcactttttagtaaagttacactACTGGTAATCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTc                                                                                                >  2:598665‑M2/95‑151 (MQ=255)
                                                 atgaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTa                                                                                              <  2:540127‑M2/59‑1 (MQ=255)
                                                   gaagttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAg                                                                                            <  2:105603‑M2/61‑1 (MQ=255)
                                                     agttttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTa                                                                                              >  1:555956‑M2/89‑147 (MQ=255)
                                                       ttttagtaacttctttaaaatcaagagcgaaaataagtaacatcaaaaataacgcggcttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAATGGTAATTTTGATGCCTAAGAtt                                                                                         >  2:956491‑M2/87‑150 (MQ=255)
                                                        tttagtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTa                                                                                        <  1:728480‑M2/65‑1 (MQ=255)
                                                           agtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAgg                                                                                    >  1:68816‑M2/83‑151 (MQ=255)
                                                            gtaacttctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGt                                                                                   <  1:620974‑M2/70‑1 (MQ=255)
                                                                  tctttaaaatcaatagctaaaataattaacatcaaaaataacgcgacttttatcactttttattaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACg                                                                               <  2:476072‑M2/74‑1 (MQ=255)
                                                                   ctttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGtt                                                                             >  1:672992‑M2/75‑150 (MQ=255)
                                                                     ttaaaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGtt                                                                             <  1:576291‑M2/76‑1 (MQ=255)
                                                                        aaatcaatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTgg                                                                       >  2:225216‑M2/70‑151 (MQ=255)
                                                                            caatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGttt                                                                    <  1:59783‑M2/85‑1 (MQ=255)
                                                                            caatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTa                                                                   <  2:911144‑M2/86‑1 (MQ=255)
                                                                             aatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGttt                                                                    >  2:251287‑M2/65‑149 (MQ=255)
                                                                             aatagctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTa                                                                   >  2:558319‑M2/65‑150 (MQ=255)
                                                                                 gctaaaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAg                                                              <  1:836406‑M2/91‑1 (MQ=255)
                                                                                     aaataagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTcc                                                           >  1:605383‑M2/57‑150 (MQ=255)
                                                                                      aataagtaacatcaaaaataacgcgacttttatcactttttattaaagtttcactACTGGTAACCGACACAGCATTTGTGTTTATTTTTCATTTAAAGGTAATTTTGATGTCTAAGATTAAAAGTAACGTTAGGTGGTTTAATGAGTCCa                                                          <  2:963036‑M2/95‑1 (MQ=255)
                                                                                        taagtaacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCaa                                                         >  1:432478‑M2/54‑149 (MQ=255)
                                                                                             aacatcaaaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTc                                                  >  1:792936‑M2/49‑151 (MQ=255)
                                                                                                    aaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTc                                            >  1:303000‑M2/42‑150 (MQ=255)
                                                                                                    aaaataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGAAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTc                                            >  2:104218‑M2/42‑150 (MQ=255)
                                                                                                       ataacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCAtt                                         >  2:474339‑M2/39‑150 (MQ=255)
                                                                                                        taacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTAc                                       >  1:558976‑M2/38‑151 (MQ=255)
                                                                                                         aacgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACt                                      >  2:198506‑M2/37‑151 (MQ=255)
                                                                                                          acgcgacttttatcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTc                                     >  1:13556‑M2/36‑151 (MQ=255)
                                                                                                                     atcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACgg                           <  1:563977‑M2/126‑1 (MQ=255)
                                                                                                                     atcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGgc                          <  2:544704‑M2/127‑1 (MQ=255)
                                                                                                                      tcactttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGgc                          <  1:233585‑M2/127‑1 (MQ=255)
                                                                                                                           ttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGgcagca                      <  2:108576‑M2/131‑1 (MQ=255)
                                                                                                                           ttttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCaa                     <  1:674050‑M2/132‑1 (MQ=255)
                                                                                                                            tttagtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCaaa                    <  1:142181‑M2/133‑1 (MQ=255)
                                                                                                                                gtaaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACgt               <  1:57957‑M2/138‑1 (MQ=255)
                                                                                                                                 taaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACgt               >  1:822826‑M2/13‑150 (MQ=255)
                                                                                                                                  aaagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACgtgt             <  2:320404‑M2/140‑1 (MQ=255)
                                                                                                                                   aagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGtt            <  1:561293‑M2/141‑1 (MQ=255)
                                                                                                                                   aagttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGtt            >  2:126034‑M2/11‑151 (MQ=255)
                                                                                                                                      ttacactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGt         >  2:3377‑M2/8‑151 (MQ=255)
                                                                                                                                       tacactACTGGTAACCGACTCAGCATTTGTGTCTATTTTTCATGTAAAGGTATTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGt         >  2:664137‑M2/7‑150 (MQ=255)
                                                                                                                                        acactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTac       <  2:432282‑M2/146‑1 (MQ=255)
                                                                                                                                         cactACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTaca      <  1:672942‑M2/147‑1 (MQ=255)
                                                                                                                                             aCTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTtc  <  2:400122/151‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                      
GATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTC  >  NC_000913/657099‑657390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.