breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
JC JC 700,602 IS1 (+) +9 bp coding (985‑993/1221 nt) nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,966,573 T→G D621A (GAT→GCT)  ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
RA 3,310,266 C→A E301D (GAG→GAT pnp ← polynucleotide phosphorylase/polyadenylase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,815,810 Δ1 bp intergenic (‑42/+24) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,378,331 A→G I205T (ATT→ACT)  ampC ← penicillin‑binding protein; beta‑lactamase, intrinsically weak

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2798606 2805673 7068 96 [0] [1] 86 alaE–[proV] alaE, ygaC, ygaM, nrdH, nrdI, nrdE, nrdF, [proV]
* * ÷ NC_000913 3423732–3424234 3424561–3424238 5–830 43 [42] [41] 43 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)75 (0.970) 66/290 0.2 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 27986050 (0.000)96 (1.210) 86/298 0.0 100% intergenic (‑110/‑559) stpA/alaE DNA binding protein, nucleoid‑associated/alanine exporter, alanine‑inducible, stress‑responsive
?NC_000913 3583428 = NA (NA)noncoding (1/768 nt) IS1 repeat region
* ? NC_000913 2805674 =1 (0.010)85 (1.070) 74/298 0.1 98.8% coding (860/1203 nt) proV glycine betaine/proline ABC transporter periplasmic binding protein
?NC_000913 = 3584195 NA (NA)noncoding (768/768 nt) IS1 repeat region