Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 3,018,356 (GTC)4→3 coding (2297‑2299/3099 nt) ygfK → putative Fe‑S subunit oxidoreductase subunit

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3018350 =1 (0.020)47 (1.160) 44/280 0.1 98.0% coding (2291/3099 nt) ygfK putative Fe‑S subunit oxidoreductase subunit
?NC_000913 = 3018346 1 (0.020)coding (2287/3099 nt) ygfK putative Fe‑S subunit oxidoreductase subunit

CGCCATTATGGTCGACGTCTGGCCAGCCGTTTTTGTCCAGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3018499‑3018350
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gacgacgacCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTTCTTCATACTCTTCGCGCCATGCGGGCATCT  <  NC_000913/3018346‑3018206
                                                                                                                                                                                                                                                                                                   
CGCCATTATGGTCGACGTCTGGCCAGCCGTTTTTGTCCAGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACC                                                                                                                                              <  1:38019/151‑1
  CCATTATGGTCGACGTCTGGCCAGCCGTTTTTGTCCAGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTT                                                                                                                                            <  1:516450/151‑1
   CATTATGGTCGACGTCTGGCCAGCCGTTTTTGTCCAGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTT                                                                                                                                           >  2:422716/1‑151
               CGTCTGGCCAGCCGTTTTTGTCCAGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTC                                                                                                                               <  2:678854/151‑1
               CGTCTGGCCAGCCGTTTTTGTCCAGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTC                                                                                                                               <  2:431863/151‑1
                   TGGCCAGCCGTTTTTGTCCAGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCC                                                                                                                            >  1:136463/1‑150
                      CCAGCCGTTTTTGTCCAGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGC                                                                                                                        <  1:622630/151‑1
                              TTTTGTCCAGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAA                                                                                                                 <  2:233918/150‑1
                                 TGTCCAGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCG                                                                                                             >  1:662310/1‑151
                                      AGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGG                                                                                                        >  2:558706/1‑151
                                      AGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGG                                                                                                        <  1:674577/151‑1
                                           CACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAG                                                                                                   <  1:422727/151‑1
                                              GCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTG                                                                                                >  2:205794/1‑151
                                                    CGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCA                                                                                          <  2:489893/151‑1
                                                      CATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGC                                                                                        <  2:412636/151‑1
                                                       ATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGC                                                                                        >  2:582783/1‑150
                                                         TCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCAT                                                                                      <  1:359323/150‑1
                                                          CAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATC                                                                                     <  1:174653/150‑1
                                                           AGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGA                                                                                   <  1:268894/151‑1
                                                               CTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACG                                                                               >  2:419703/1‑151
                                                                 TCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACG                                                                               <  2:569775/149‑1
                                                                  CAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTT                                                                             <  1:394911/150‑1
                                                                   AGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCC                                                                           <  1:295362/151‑1
                                                                      ATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGG                                                                         <  1:331068/150‑1
                                                                       TCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGA                                                                        <  2:237152/150‑1
                                                                           GCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATT                                                                   >  1:611881/1‑151
                                                                              GTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCA                                                                 <  1:56913/150‑1
                                                                                    CAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACG                                                          <  1:47469/151‑1
                                                                                            GTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTA                                                  <  1:391782/151‑1
                                                                                                   GGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTC                                           >  2:522556/1‑151
                                                                                                       GTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGC                                       >  1:182223/1‑151
                                                                                                           ACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACG                                   <  1:358020/151‑1
                                                                                                           ACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAA                                     <  2:262839/149‑1
                                                                                                            CAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGC                                  <  1:265779/151‑1
                                                                                                             AAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCT                                 >  1:393042/1‑151
                                                                                                              AGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTT                                <  2:189150/151‑1
                                                                                                              AGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTT                                <  2:135497/151‑1
                                                                                                               GCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTTC                               <  1:53420/151‑1
                                                                                                                         TGTTTCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTTCTTCATACTCT                     >  2:555876/1‑151
                                                                                                                             TCATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTTCTTCATACTCTTCGC                 >  2:60328/1‑151
                                                                                                                              CATTGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTTCTTCATACTCTTCGCG                <  2:491673/151‑1
                                                                                                                                 TGGTTTCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTTCTTCATACTCTTCGCGCC              >  1:92890/1‑150
                                                                                                                                      TCAACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTTCTTCATACTCTTCGCGCCATGCGG        >  1:552815/1‑151
                                                                                                                                        AACCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTTCTTCATACTCTTCGCGCCATGCGGGC      >  2:449087/1‑151
                                                                                                                                          CCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTTCTTCATACTCTTCGCGCCATGCGGGCAT    >  1:241473/1‑151
                                                                                                                                          CCGGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTTCTTCATACTCTTCGCGCCATGCGGGCAT    >  1:590476/1‑151
                                                                                                                                            GGACGACGACCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTTCTTCATACTCTTCGCGCCATGCGGGCATCT  <  2:70826/151‑1
                                                                                                                                                                                                                                                                                                   
CGCCATTATGGTCGACGTCTGGCCAGCCGTTTTTGTCCAGCGGCACGCCCATCGCATTCAGGGCTTCAGTATCCTGCTGTTCACCAATGGCGGTGATCAGGCTGTCTACAAGCAGTGTTACTGTTTCATTGGTTTCAACCGGACGACGAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3018499‑3018350
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gacgacgacCTTTCTCATCCGGTTCGCCAAGCGACATAACGCGCAAGGTTAAGGTGCCATCAGCATCGAAACGTTCCGGATTATTCAGGAAACGGAACTCTACGCCGTCGTGCAACGCTTCTTCATACTCTTCGCGCCATGCGGGCATCT  <  NC_000913/3018346‑3018206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.