Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 3,018,356 (GTC)4→3 coding (2297‑2299/3099 nt) ygfK → putative Fe‑S subunit oxidoreductase subunit

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3018350 =1 (0.020)47 (1.160) 44/280 0.1 98.0% coding (2291/3099 nt) ygfK putative Fe‑S subunit oxidoreductase subunit
?NC_000913 = 3018346 1 (0.020)coding (2287/3099 nt) ygfK putative Fe‑S subunit oxidoreductase subunit

AGATGCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATG  >  NC_000913/3018206‑3018496
                                                                                                                                            |                                                                                                                                                      
agaTGCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtcc                                                                                                                                              <  2:70826‑M2/151‑11 (MQ=255)
  aTGCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccgg                                                                                                                                            >  1:241473‑M2/1‑139 (MQ=255)
  aTGCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccgg                                                                                                                                            >  1:590476‑M2/1‑139 (MQ=255)
    gCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggtt                                                                                                                                          >  2:449087‑M2/1‑137 (MQ=255)
      ccGCATGGCGCGAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttga                                                                                                                                        >  1:552815‑M2/1‑135 (MQ=255)
          aTGGCGCGAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGaa                                                                                                                                    <  1:112160/151‑1 (MQ=255)
            ggCGCGAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaacca                                                                                                                                   >  1:92890‑M2/1‑129 (MQ=255)
              cgcgAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatg                                                                                                                                <  2:491673‑M2/151‑25 (MQ=255)
               gcgAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatga                                                                                                                               >  2:60328‑M2/1‑126 (MQ=255)
                   agagTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaaca                                                                                                                           >  2:555876‑M2/1‑122 (MQ=255)
                             gaagCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgc                                                                                                                 <  1:53420‑M2/151‑40 (MQ=255)
                              aagCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgct                                                                                                                <  2:189150‑M2/151‑41 (MQ=255)
                              aagCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgct                                                                                                                <  2:135497‑M2/151‑41 (MQ=255)
                               agCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgctt                                                                                                               >  1:393042‑M2/1‑110 (MQ=255)
                                gCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttg                                                                                                              <  1:265779‑M2/151‑43 (MQ=255)
                                 cGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgt                                                                                                             <  1:358020‑M2/151‑44 (MQ=255)
                                   ttGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgt                                                                                                             <  2:262839‑M2/149‑44 (MQ=255)
                                     gCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagac                                                                                                         >  1:182223‑M2/1‑104 (MQ=255)
                                         gacgGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcc                                                                                                     >  2:522556‑M2/1‑100 (MQ=255)
                                                tAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcac                                                                                              <  1:391782‑M2/151‑59 (MQ=255)
                                                        cGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattg                                                                                      <  1:47469‑M2/151‑67 (MQ=255)
                                                               tGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaac                                                                                <  1:56913‑M2/150‑73 (MQ=255)
                                                                 aataatCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagc                                                                             >  1:611881‑M2/1‑76 (MQ=255)
                                                                      tCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcagga                                                                         <  2:237152‑M2/150‑80 (MQ=255)
                                                                       ccGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggat                                                                        <  1:331068‑M2/150‑81 (MQ=255)
                                                                         ggAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatact                                                                     <  1:295362‑M2/151‑84 (MQ=255)
                                                                           aaCGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactg                                                                    <  1:394911‑M2/150‑85 (MQ=255)
                                                                             cGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaag                                                                 >  2:419703‑M2/1‑64 (MQ=255)
                                                                             cGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactga                                                                   <  2:569775‑M2/149‑86 (MQ=255)
                                                                                 tCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccct                                                             <  1:268894‑M2/151‑92 (MQ=255)
                                                                                   gATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctg                                                            <  1:174653‑M2/150‑93 (MQ=255)
                                                                                    aTGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctga                                                           <  1:359323‑M2/150‑94 (MQ=255)
                                                                                      gCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatg                                                        <  2:412636‑M2/151‑97 (MQ=255)
                                                                                      gCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaat                                                         >  2:582783‑M2/1‑55 (MQ=255)
                                                                                        tGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcg                                                      <  2:489893‑M2/151‑99 (MQ=255)
                                                                                              cACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggc                                                >  2:205794‑M2/1‑47 (MQ=255)
                                                                                                 cTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggcgtg                                             <  1:422727‑M2/151‑108 (MQ=255)
                                                                                                      ccTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggcgtgccgct                                        >  2:558706‑M2/1‑39 (MQ=255)
                                                                                                      ccTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggcgtgccgct                                        <  1:674577‑M2/151‑113 (MQ=255)
                                                                                                           cgcgTTATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggcgtgccgctggaca                                   >  1:662310‑M2/1‑34 (MQ=255)
                                                                                                               ttATGTCGCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggcgtgccgctggacaaaa                                <  2:233918‑M2/150‑121 (MQ=255)
                                                                                                                      gCTTGGCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggcgtgccgctggacaaaaacggctgg                        <  1:622630‑M2/151‑129 (MQ=255)
                                                                                                                          ggCGAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggcgtgccgctggacaaaaacggctggcca                     >  1:136463‑M2/1‑19 (MQ=255)
                                                                                                                             gAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggcgtgccgctggacaaaaacggctggccagacg                 <  2:431863‑M2/151‑136 (MQ=255)
                                                                                                                             gAACCGGATGAGAAAGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggcgtgccgctggacaaaaacggctggccagacg                 <  2:678854‑M2/151‑136 (MQ=255)
                                                                                                                                         aaaGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggcgtgccgctggacaaaaacggctggccagacgtcgaccataatg     >  2:422716‑M2/1‑4 (MQ=255)
                                                                                                                                          aaGgtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggcgtgccgctggacaaaaacggctggccagacgtcgaccataatgg    <  1:516450‑M2/151‑149 (MQ=255)
                                                                                                                                            ggtcgtcgtccggttgaaaccaatgaaacagtaacactgcttgtagacagcctgatcaccgccattggtgaacagcaggatactgaagccctgaatgcgatgggcgtgccgctggacaaaaacggctggccagacgtcgaccataatggcg  <  1:38019‑M2/151‑151 (MQ=255)
                                                                                                                                            |                                                                                                                                                      
AGATGCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATG  >  NC_000913/3018206‑3018496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.