Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 3,018,356 (GTC)4→3 coding (2297‑2299/3099 nt) ygfK → putative Fe‑S subunit oxidoreductase subunit

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3018350 =1 (0.020)47 (1.160) 44/280 0.1 98.0% coding (2291/3099 nt) ygfK putative Fe‑S subunit oxidoreductase subunit
?NC_000913 = 3018346 1 (0.020)coding (2287/3099 nt) ygfK putative Fe‑S subunit oxidoreductase subunit

TGCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCG  >  NC_000913/3018209‑3018499
                                                                                                                                             |                                                                                                                                                     
agatgcccgcatggcgcgaagagtatgaagaagcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTcc                                                                                                                                              <  2:70826‑M1/10‑1 (MQ=255)
  atgcccgcatggcgcgaagagtatgaagaagcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCgg                                                                                                                                            >  1:241473‑M1/140‑151 (MQ=255)
  atgcccgcatggcgcgaagagtatgaagaagcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCgg                                                                                                                                            >  1:590476‑M1/140‑151 (MQ=255)
    gcccgcatggcgcgaagagtatgaagaagcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGtt                                                                                                                                          >  2:449087‑M1/138‑151 (MQ=255)
      ccgcatggcgcgaagagtatgaagaagcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGa                                                                                                                                        >  1:552815‑M1/136‑151 (MQ=255)
       aTGGCGCGAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGaa                                                                                                                                       <  1:112160/151‑1 (MQ=255)
            ggcgcgaagagtatgaagaagcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCa                                                                                                                                   >  1:92890‑M1/130‑150 (MQ=255)
              cgcgaagagtatgaagaagcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATg                                                                                                                                <  2:491673‑M1/24‑1 (MQ=255)
               gcgaagagtatgaagaagcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGa                                                                                                                               >  2:60328‑M1/127‑151 (MQ=255)
                   agagtatgaagaagcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACa                                                                                                                           >  2:555876‑M1/123‑151 (MQ=255)
                             gaagcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGc                                                                                                                 <  1:53420‑M1/39‑1 (MQ=255)
                              aagcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCt                                                                                                                <  2:189150‑M1/40‑1 (MQ=255)
                              aagcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCt                                                                                                                <  2:135497‑M1/40‑1 (MQ=255)
                               agcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCtt                                                                                                               >  1:393042‑M1/111‑151 (MQ=255)
                                gcgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTg                                                                                                              <  1:265779‑M1/42‑1 (MQ=255)
                                 cgttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGt                                                                                                             <  1:358020‑M1/43‑1 (MQ=255)
                                   ttgcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGt                                                                                                             <  2:262839‑M1/43‑1 (MQ=255)
                                     gcacgacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGAc                                                                                                         >  1:182223‑M1/105‑151 (MQ=255)
                                         gacggcgtagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGcc                                                                                                     >  2:522556‑M1/101‑151 (MQ=255)
                                                tagagttccgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCAc                                                                                              <  1:391782‑M1/58‑1 (MQ=255)
                                                        cgtttcctgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTg                                                                                      <  1:47469‑M1/66‑1 (MQ=255)
                                                               tgaataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAAc                                                                                <  1:56913‑M1/72‑1 (MQ=255)
                                                                 aataatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAAcagc                                                                             >  1:611881‑M1/77‑151 (MQ=255)
                                                                      tccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGa                                                                         <  2:237152‑M1/79‑1 (MQ=255)
                                                                       ccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGAt                                                                        <  1:331068‑M1/80‑1 (MQ=255)
                                                                         ggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACt                                                                     <  1:295362‑M1/83‑1 (MQ=255)
                                                                           aacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTg                                                                    <  1:394911‑M1/84‑1 (MQ=255)
                                                                             cgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGa                                                                   <  2:569775‑M1/85‑1 (MQ=255)
                                                                             cgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAg                                                                 >  2:419703‑M1/65‑151 (MQ=255)
                                                                                 tcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCt                                                             <  1:268894‑M1/91‑1 (MQ=255)
                                                                                   gatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTg                                                            <  1:174653‑M1/92‑1 (MQ=255)
                                                                                    atgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGa                                                           <  1:359323‑M1/93‑1 (MQ=255)
                                                                                      gctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAAt                                                         >  2:582783‑M1/56‑150 (MQ=255)
                                                                                      gctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATg                                                        <  2:412636‑M1/96‑1 (MQ=255)
                                                                                        tgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCg                                                      <  2:489893‑M1/98‑1 (MQ=255)
                                                                                              caccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGc                                                >  2:205794‑M1/48‑151 (MQ=255)
                                                                                                 cttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTg                                             <  1:422727‑M1/107‑1 (MQ=255)
                                                                                                      ccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCt                                        >  2:558706‑M1/40‑151 (MQ=255)
                                                                                                      ccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCt                                        <  1:674577‑M1/112‑1 (MQ=255)
                                                                                                           cgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACa                                   >  1:662310‑M1/35‑151 (MQ=255)
                                                                                                               ttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACaaaa                                <  2:233918‑M1/120‑1 (MQ=255)
                                                                                                                      gcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTgg                        <  1:622630‑M1/128‑1 (MQ=255)
                                                                                                                          ggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCa                     >  1:136463‑M1/20‑150 (MQ=255)
                                                                                                                             gaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACg                 <  2:431863‑M1/135‑1 (MQ=255)
                                                                                                                             gaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACg                 <  2:678854‑M1/135‑1 (MQ=255)
                                                                                                                                         aaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATg     >  2:422716‑M1/5‑151 (MQ=255)
                                                                                                                                          aagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATgg    <  1:516450‑M1/148‑1 (MQ=255)
                                                                                                                                            gGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCg  <  1:38019‑M1/150‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                     
TGCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCACGACGGCGTAGAGTTCCGTTTCCTGAATAATCCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCG  >  NC_000913/3018209‑3018499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.