Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 3,970,427 (ACG)4→3 coding (295‑297/1131 nt) wecB → UDP‑N‑acetyl glucosamine‑2‑epimerase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3970421 =1 (0.030)28 (0.930) 20/204 0.4 96.7% coding (289/1131 nt) wecB UDP‑N‑acetyl glucosamine‑2‑epimerase
?NC_000913 = 3970417 1 (0.030)coding (285/1131 nt) wecB UDP‑N‑acetyl glucosamine‑2‑epimerase

GTCAATGTACGGTTAGCCTCTTCCGGCCACGGCGAATAGAGATCGCCCGTGCGCAGACCAGCCTCAACGTGACCAACAGGAATACGCTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3970545‑3970421
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgtcgtcgtATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGCCCTGTCCTGGCTGCATTATGTTGAGATCGTAGTCAGGTA  <  NC_000913/3970417‑3970290
                                                                                                                                                                                                                                                             
GTCAATGTACGGTTAGCCTCTTCCGGCCACGGCGAATAGAGATCGCCCGTGCGCAGACCAGCCTCAACGTGACCAACAGGAATACGCTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCA                                                                                                                    >  1:704685/1‑139
               GCCTCTTCCGGCCACGGCGAATAGAGATCGCCCGTGCGCAGACCAGCCTCAACGTGACCAACAGGAATACGCTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTT                                                                                                     >  2:667331/1‑139
               GCCTCTTCCGGCCACGGCGAATAGAGATCGCCCGTGCGCAGACCAGCCTCAACGTGACCAACAGGAATACGCTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTT                                                                                                     >  2:363601/1‑139
               GCCTCTTCCGGCCACGGCGAATAGAGATCGCCCGTGCGCAGACCAGCCTCAACGTGACCAACAGGAATACGCTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTT                                                                                                     >  1:641575/1‑139
               GCCTCTTCCGGCCACGGCGAATAGAGATCGCCCGTGCGCAGACCAGCCTCAACGTGACCAACAGGAATACGCTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTT                                                                                                     >  1:60771/1‑139
                    TTCCGGCCACGGCGAATAGAGATCGCCCGTGCGCAGACCAGCCTCAACGTGACCAACAGGAATACGCTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAAC                                                                                                >  1:512091/1‑139
                       CGGCCACGGCGAATAGAGATCGCCCGTGCGCAGACCAGCCTCAACGTGACCAACAGGAATACGCTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCG                                                                                             <  1:460966/139‑1
                                             CCCGTGCGCAGACCAGCCTCAACGTGACCAACAGGAATACGCTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTT                                                                       >  1:564196/1‑139
                                                                CAACGTGACCAACAGGAATACGCTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTAT                                                    <  2:564196/139‑1
                                                                                AATACGCTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGCCCTGT                                    <  2:704685/139‑1
                                                                                      CTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGCCCTGTCCTGGC                              >  1:509188/1‑139
                                                                                      CTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGT                                                       <  1:184714/114‑1
                                                                                      CTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGT                                                       >  2:184714/1‑114
                                                                                       TGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGCCCTGTCCTGGCT                             >  1:353300/1‑139
                                                                                       TGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTC                                             >  2:581806/1‑123
                                                                                       TGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTC                                             <  1:581806/123‑1
                                                                                         ATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGC                                                                                           <  2:462228/75‑1
                                                                                         ATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGC                                                                                           >  1:462228/1‑75
                                                                                          TAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGCCCTGTCCTGGCTGCA                          <  1:363601/139‑1
                                                                                          TAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGCCCTGTCCTGGCTGCA                          <  1:667331/139‑1
                                                                                            AAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGCCCTGTCCTGGCTGCATT                        <  2:547872/139‑1
                                                                                                  CGCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGCCCTGTCCTGGCTGCATTATGTTG                  <  2:353300/139‑1
                                                                                                   GCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCAC                                                                                                                <  1:426068/44‑1
                                                                                                   GCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCAC                                                                                                                >  2:426068/1‑44
                                                                                                   GCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCAC                                                                                                                >  2:521258/1‑44
                                                                                                   GCCAGGCTGGTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCAC                                                                                                                <  1:521258/44‑1
                                                                                                            GTTGCCAGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGCCCTGTCCTGGCTGCATTATGTTGAGATCGTAGT        >  2:646487/1‑139
                                                                                                                  AGCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGCCCTGTCCTGGCTGCATTATGTTGAGATCGTAGTCAGGTA  <  2:641575/139‑1
                                                                                                                                                                                                                                                             
GTCAATGTACGGTTAGCCTCTTCCGGCCACGGCGAATAGAGATCGCCCGTGCGCAGACCAGCCTCAACGTGACCAACAGGAATACGCTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3970545‑3970421
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgtcgtcgtATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGCCCTGTCCTGGCTGCATTATGTTGAGATCGTAGTCAGGTA  <  NC_000913/3970417‑3970290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.