Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 3,970,427 (ACG)4→3 coding (295‑297/1131 nt) wecB → UDP‑N‑acetyl glucosamine‑2‑epimerase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3970421 =1 (0.030)28 (0.930) 20/204 0.4 96.7% coding (289/1131 nt) wecB UDP‑N‑acetyl glucosamine‑2‑epimerase
?NC_000913 = 3970417 1 (0.030)coding (285/1131 nt) wecB UDP‑N‑acetyl glucosamine‑2‑epimerase

TACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTACATT  >  NC_000913/3970290‑3970542
                                                                                                                               |                                                                                                                             
tACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgct                                                                                                                    <  2:641575‑M2/139‑12 (MQ=255)
      aCTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaac                                                                                                              >  2:646487‑M2/1‑122 (MQ=255)
                cAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcg                                                                                                    <  2:353300‑M2/139‑28 (MQ=255)
                      aaTGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttt                                                                                              <  2:547872‑M2/139‑34 (MQ=255)
                        tGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgtttta                                                                                            <  1:363601‑M2/139‑36 (MQ=255)
                        tGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgtttta                                                                                            <  1:667331‑M2/139‑36 (MQ=255)
                           aGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatca                                                                                         >  1:353300‑M2/1‑101 (MQ=255)
                            gCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcag                                                                                        >  1:509188‑M2/1‑100 (MQ=255)
                                  aCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtatt                                                                                  <  2:704685‑M2/139‑46 (MQ=255)
                                           gACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatca                                                                                         <  1:581806‑M2/123‑39 (MQ=255)
                                           gACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatca                                                                                         >  2:581806‑M2/1‑85 (MQ=255)
                                                  aTAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttg                                                                  <  2:564196‑M2/139‑62 (MQ=255)
                                                     aCCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcag                                                                                        <  1:184714‑M2/114‑40 (MQ=255)
                                                     aCCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcag                                                                                        >  2:184714‑M2/1‑75 (MQ=255)
                                                                     aaGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacggg                                               >  1:564196‑M2/1‑59 (MQ=255)
                                                                                         gCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttat                                                                                           <  2:462228‑M2/75‑37 (MQ=255)
                                                                                         gCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttat                                                                                           >  1:462228‑M2/1‑39 (MQ=255)
                                                                                           cGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccg                         <  1:460966‑M2/139‑103 (MQ=255)
                                                                                              gTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaa                      >  1:512091‑M2/1‑34 (MQ=255)
                                                                                                 cAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGaa                   <  2:321608/139‑1 (MQ=255)
                                                                                                   aaCCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggc                 >  2:363601‑M2/1‑29 (MQ=255)
                                                                                                   aaCCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggc                 >  1:641575‑M2/1‑29 (MQ=255)
                                                                                                   aaCCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggc                 >  1:60771‑M2/1‑29 (MQ=255)
                                                                                                   aaCCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggc                 >  2:667331‑M2/1‑29 (MQ=255)
                                                                                                              gtGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                     <  1:521258‑M2/44‑27 (MQ=255)
                                                                                                              gtGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                     >  2:426068‑M2/1‑18 (MQ=255)
                                                                                                              gtGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                     >  2:521258‑M2/1‑18 (MQ=255)
                                                                                                              gtGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                     <  1:426068‑M2/44‑27 (MQ=255)
                                                                                                                  tGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggctaaccgtacattgac  >  1:704685‑M2/1‑14 (MQ=255)
                                                                                                                               |                                                                                                                             
TACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTACATT  >  NC_000913/3970290‑3970542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.