| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913 | 3,970,427 | (ACG)4→3 | coding (295‑297/1131 nt) | wecB → | UDP‑N‑acetyl glucosamine‑2‑epimerase |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 3970421 = | 1 (0.030) | 28 (0.930) | 20/204 | 0.4 | 96.7% | coding (289/1131 nt) | wecB | UDP‑N‑acetyl glucosamine‑2‑epimerase |
| ? | NC_000913 | = 3970417 | 1 (0.030) | coding (285/1131 nt) | wecB | UDP‑N‑acetyl glucosamine‑2‑epimerase | |||||
CTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTACATTGAC > NC_000913/3970293‑3970545 | tacctgactacgatctcaacataatgcagccaggacagggcctgacagagataacctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCt < 2:641575‑M1/11‑1 (MQ=255) actacgatctcaacataatgcagccaggacagggcctgacagagataacctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAAc > 2:646487‑M1/123‑139 (MQ=255) caacataatgcagccaggacagggcctgacagagataacctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTggcg < 2:353300‑M1/27‑1 (MQ=255) aatgcagccaggacagggcctgacagagataacctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGttt < 2:547872‑M1/33‑1 (MQ=255) tgcagccaggacagggcctgacagagataacctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTa < 1:363601‑M1/35‑1 (MQ=255) tgcagccaggacagggcctgacagagataacctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTa < 1:667331‑M1/35‑1 (MQ=255) agccaggacagggcctgacagagataacctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCa > 1:353300‑M1/102‑139 (MQ=255) gccaggacagggcctgacagagataacctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAg > 1:509188‑M1/101‑139 (MQ=255) acagggcctgacagagataacctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTAtt < 2:704685‑M1/45‑1 (MQ=255) gacagagataacctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCa < 1:581806‑M1/38‑1 (MQ=255) gacagagataacctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCa > 2:581806‑M1/86‑123 (MQ=255) ataacctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTg < 2:564196‑M1/61‑1 (MQ=255) acctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAg < 1:184714‑M1/39‑1 (MQ=255) acctgtcggattctggaagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAg > 2:184714‑M1/76‑114 (MQ=255) aagggctaaaacctattcttgccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACggg > 1:564196‑M1/60‑139 (MQ=255) gccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTAt < 2:462228‑M1/36‑1 (MQ=255) gccgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTAt > 1:462228‑M1/40‑75 (MQ=255) cgagttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCg < 1:460966‑M1/102‑1 (MQ=255) gttcaaaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGaa > 1:512091‑M1/35‑139 (MQ=255) cAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGaa < 2:321608/139‑1 (MQ=255) aaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGc > 2:363601‑M1/30‑139 (MQ=255) aaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGc > 1:641575‑M1/30‑139 (MQ=255) aaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGc > 1:60771‑M1/30‑139 (MQ=255) aaccagacgtcgtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGc > 2:667331‑M1/30‑139 (MQ=255) gtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTggc < 1:521258‑M1/26‑1 (MQ=255) gtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTggc > 2:426068‑M1/19‑44 (MQ=255) gtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTggc > 2:521258‑M1/19‑44 (MQ=255) gtgctggttcacggcgatACGACGACGCTGGCAACCAGCCTggc < 1:426068‑M1/26‑1 (MQ=255) tggttcacggcgatACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTACATTGAc > 1:704685‑M1/15‑139 (MQ=255) | CTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTACATTGAC > NC_000913/3970293‑3970545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |