breseq version 0.27.1 revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 90 shown) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 357,880 | 0 | T | C | 69.2% | ‑3.4 / 13.0 | 26 | K271E (AAA→GAA) | cynR | transcriptional activator of cyn operon; autorepressor |
* | NC_000913 | 2,442,632 | 0 | T | G | 63.6% | 14.6 / 21.4 | 22 | V290G (GTA→GGA) | mnmC | fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
* | NC_000913 | 357,890 | 0 | C | A | 62.5% | ‑1.5 / 12.9 | 24 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
* | NC_000913 | 1,356,201 | 0 | T | G | 60.0% | 27.9 / 35.4 | 26 | T304P (ACC→CCC) | sapA | antimicrobial peptide transport ABC transporter periplasmic binding protein |
* | NC_000913 | 3,177,531 | 0 | A | C | 58.6% | 14.0 / 28.5 | 30 | V127G (GTG→GGG) | nudF | ADP‑ribose pyrophosphatase |
* | NC_000913 | 328,791 | 0 | A | G | 57.7% | 4.4 / 24.5 | 26 | S182P (TCC→CCC) | betI | choline‑inducible betIBA‑betT divergent operon transcriptional repressor |
* | NC_000913 | 3,953,550 | 0 | A | C | 56.7% | 21.5 / 42.2 | 30 | T25P (ACC→CCC) | ilvD | dihydroxyacid dehydratase |
* | NC_000913 | 328,803 | 0 | T | G | 56.0% | ‑2.3 / 29.3 | 25 | T178P (ACC→CCC) | betI | choline‑inducible betIBA‑betT divergent operon transcriptional repressor |
* | NC_000913 | 328,807 | 0 | A | T | 56.0% | 5.2 / 28.0 | 25 | D176E (GAT→GAA) | betI | choline‑inducible betIBA‑betT divergent operon transcriptional repressor |
* | NC_000913 | 2,386,862 | 0 | G | A | 54.8% | 0.1 / 52.1 | 42 | intergenic (+31/‑72) | yfbL/yfbM | putative M28A family peptidase/DUF1877 family protein |
* | NC_000913 | 993,913 | 0 | G | C | 53.3% | 33.6 / 50.5 | 30 | G44G (GGC→GGG) | ssuB | aliphatic sulfonate ABC transporter ATPase |
* | NC_000913 | 328,816 | 0 | T | G | 52.0% | 30.1 / 38.0 | 25 | K173N (AAA→AAC) | betI | choline‑inducible betIBA‑betT divergent operon transcriptional repressor |
* | NC_000913 | 3,556,207 | 0 | T | C | 51.8% | 15.9 / 35.9 | 28 | S215G (AGT→GGT) | rtcA | RNA 3'‑terminal phosphate cyclase |
* | NC_000913 | 4,346,675 | 0 | T | G | 51.6% | 33.9 / 42.8 | 31 | T218P (ACC→CCC) | fumB | anaerobic class I fumarate hydratase (fumarase B) |
* | NC_000913 | 3,578,290 | 0 | A | C | 51.2% | 47.1 / 63.2 | 44 | V146G (GTG→GGG) | gntR | d‑gluconate inducible gluconate regulon transcriptional repressor |
* | NC_000913 | 3,019,596 | 0 | G | A | 48.6% | 24.7 / 18.1 | 39 | V146I (GTT→ATT) | ssnA | putative chlorohydrolase/aminohydrolase |
* | NC_000913 | 4,465,787 | 0 | G | A | 48.4% | 27.4 / 11.2 | 31 | R132C (CGC→TGC) | treB | trehalose‑specific PTS enzyme: IIB and IIC component |
* | NC_000913 | 75,079 | 0 | C | T | 48.1% | 14.6 / 16.8 | 27 | L134L (CTG→CTA) | thiB | thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein |
* | NC_000913 | 4,538,109 | 0 | A | C | 47.9% | 62.4 / 13.7 | 48 | G219G (GGT→GGG) | nanM | N‑acetylneuraminic acid mutarotase |
* | NC_000913 | 2,275,810 | 0 | A | T | 47.1% | 35.9 / 16.1 | 34 | I180F (ATT→TTT) | yejE | microcin C ABC transporter permease |
Marginal new junction evidence (lowest skew 10 of 92 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1616905 | 1222 (28.000) | 28 (0.690) | 17/278 | 0.8 | 2.3% | intergenic (+27/‑123) | yneK/ydeA | uncharacterized protein/arabinose efflux transporter, arabinose‑inducible |
? | NC_000913 | = 1616917 | 1224 (30.080) | intergenic (+39/‑111) | yneK/ydeA | uncharacterized protein/arabinose efflux transporter, arabinose‑inducible | |||||
* | ? | NC_000913 | 1616896 = | 1223 (28.030) | 17 (0.420) | 14/278 | 1.1 | 1.4% | intergenic (+18/‑132) | yneK/ydeA | uncharacterized protein/arabinose efflux transporter, arabinose‑inducible |
? | NC_000913 | 1616928 = | 1224 (30.080) | intergenic (+50/‑100) | yneK/ydeA | uncharacterized protein/arabinose efflux transporter, arabinose‑inducible | |||||
* | ? | NC_000913 | = 2713829 | 44 (1.010) | 10 (0.250) | 9/276 | 1.8 | 19.5% | coding (934/1335 nt) | srmB | ATP‑dependent RNA helicase |
? | NC_000913 | = 2713908 | 42 (1.040) | coding (1013/1335 nt) | srmB | ATP‑dependent RNA helicase | |||||
* | ? | NC_000913 | = 1622333 | 1350 (30.940) | 12 (0.290) | 7/282 | 2.3 | 0.85% | coding (1002/1188 nt) | ydeE | putative transporter |
? | NC_000913 | = 1622355 | 1507 (36.510) | coding (1024/1188 nt) | ydeE | putative transporter | |||||
* | ? | NC_000913 | 1618222 = | 1811 (41.500) | 6 (0.150) | 6/282 | 2.6 | 0.36% | intergenic (+4/+21) | ydeA/marC | arabinose efflux transporter, arabinose‑inducible/UPF0056 family inner membrane protein |
? | NC_000913 | 1618242 = | 1613 (39.080) | intergenic (+24/+1) | ydeA/marC | arabinose efflux transporter, arabinose‑inducible/UPF0056 family inner membrane protein | |||||
* | ? | NC_000913 | = 734250 | 50 (1.150) | 10 (0.240) | 6/282 | 2.6 | 17.4% | pseudogene (118/1521 nt) | rhsO | pseudogene, Rhs family |
? | NC_000913 | = 3622011 | NA (NA) | coding (2820/4236 nt) | rhsB | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor | |||||
* | ? | NC_000913 | = 4318528 | 34 (0.780) | 5 (0.120) | 5/280 | 2.8 | 13.2% | coding (237/759 nt) | phnK | carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein |
? | NC_000913 | = 4318586 | 34 (0.830) | coding (179/759 nt) | phnK | carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein | |||||
* | ? | NC_000913 | = 3620868 | NA (NA) | 5 (0.120) | 5/276 | 2.8 | 13.1% | coding (1677/4236 nt) | rhsB | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor |
? | NC_000913 | = 3621008 | 36 (0.830) | coding (1817/4236 nt) | rhsB | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor | |||||
* | ? | NC_000913 | = 3544651 | 31 (0.710) | 5 (0.120) | 5/280 | 2.8 | 14.5% | coding (194/771 nt) | bioH | pimeloyl‑ACP methyl ester carboxylesterase |
? | NC_000913 | = 3544692 | 30 (0.730) | coding (153/771 nt) | bioH | pimeloyl‑ACP methyl ester carboxylesterase | |||||
* | ? | NC_000913 | = 3340296 | 48 (1.100) | 5 (0.120) | 5/280 | 2.8 | 9.6% | coding (22/978 nt) | yrbG | putative calcium/sodium:proton antiporter |
? | NC_000913 | = 3340323 | 49 (1.200) | coding (49/978 nt) | yrbG | putative calcium/sodium:proton antiporter |