breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 90 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8800TC69.2% ‑3.4 / 13.0 26K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
*NC_0009132,442,6320TG63.6% 14.6 / 21.4 22V290G (GTA→GGA) mnmCfused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
*NC_000913357,8900CA62.5% ‑1.5 / 12.9 24L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_0009131,356,2010TG60.0% 27.9 / 35.4 26T304P (ACC→CCC) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
*NC_0009133,177,5310AC58.6% 14.0 / 28.5 30V127G (GTG→GGG) nudFADP‑ribose pyrophosphatase
*NC_000913328,7910AG57.7% 4.4 / 24.5 26S182P (TCC→CCC) betIcholine‑inducible betIBA‑betT divergent operon transcriptional repressor
*NC_0009133,953,5500AC56.7% 21.5 / 42.2 30T25P (ACC→CCC) ilvDdihydroxyacid dehydratase
*NC_000913328,8030TG56.0% ‑2.3 / 29.3 25T178P (ACC→CCC) betIcholine‑inducible betIBA‑betT divergent operon transcriptional repressor
*NC_000913328,8070AT56.0% 5.2 / 28.0 25D176E (GAT→GAAbetIcholine‑inducible betIBA‑betT divergent operon transcriptional repressor
*NC_0009132,386,8620GA54.8% 0.1 / 52.1 42intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
*NC_000913993,9130GC53.3% 33.6 / 50.5 30G44G (GGC→GGGssuBaliphatic sulfonate ABC transporter ATPase
*NC_000913328,8160TG52.0% 30.1 / 38.0 25K173N (AAA→AACbetIcholine‑inducible betIBA‑betT divergent operon transcriptional repressor
*NC_0009133,556,2070TC51.8% 15.9 / 35.9 28S215G (AGT→GGT) rtcARNA 3'‑terminal phosphate cyclase
*NC_0009134,346,6750TG51.6% 33.9 / 42.8 31T218P (ACC→CCC) fumBanaerobic class I fumarate hydratase (fumarase B)
*NC_0009133,578,2900AC51.2% 47.1 / 63.2 44V146G (GTG→GGG) gntRd‑gluconate inducible gluconate regulon transcriptional repressor
*NC_0009133,019,5960GA48.6% 24.7 / 18.1 39V146I (GTT→ATT) ssnAputative chlorohydrolase/aminohydrolase
*NC_0009134,465,7870GA48.4% 27.4 / 11.2 31R132C (CGC→TGC) treBtrehalose‑specific PTS enzyme: IIB and IIC component
*NC_00091375,0790CT48.1% 14.6 / 16.8 27L134L (CTG→CTAthiBthiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
*NC_0009134,538,1090AC47.9% 62.4 / 13.7 48G219G (GGT→GGGnanMN‑acetylneuraminic acid mutarotase
*NC_0009132,275,8100AT47.1% 35.9 / 16.1 34I180F (ATT→TTT) yejEmicrocin C ABC transporter permease

Marginal new junction evidence (lowest skew 10 of 92 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 16169051222 (28.000)28 (0.690) 17/278 0.8 2.3% intergenic (+27/‑123) yneK/ydeA uncharacterized protein/arabinose efflux transporter, arabinose‑inducible
?NC_000913 = 1616917 1224 (30.080)intergenic (+39/‑111) yneK/ydeA uncharacterized protein/arabinose efflux transporter, arabinose‑inducible
* ? NC_000913 1616896 =1223 (28.030)17 (0.420) 14/278 1.1 1.4% intergenic (+18/‑132) yneK/ydeA uncharacterized protein/arabinose efflux transporter, arabinose‑inducible
?NC_000913 1616928 = 1224 (30.080)intergenic (+50/‑100) yneK/ydeA uncharacterized protein/arabinose efflux transporter, arabinose‑inducible
* ? NC_000913 = 271382944 (1.010)10 (0.250) 9/276 1.8 19.5% coding (934/1335 nt) srmB ATP‑dependent RNA helicase
?NC_000913 = 2713908 42 (1.040)coding (1013/1335 nt) srmB ATP‑dependent RNA helicase
* ? NC_000913 = 16223331350 (30.940)12 (0.290) 7/282 2.3 0.85% coding (1002/1188 nt) ydeE putative transporter
?NC_000913 = 1622355 1507 (36.510)coding (1024/1188 nt) ydeE putative transporter
* ? NC_000913 1618222 =1811 (41.500)6 (0.150) 6/282 2.6 0.36% intergenic (+4/+21) ydeA/marC arabinose efflux transporter, arabinose‑inducible/UPF0056 family inner membrane protein
?NC_000913 1618242 = 1613 (39.080)intergenic (+24/+1) ydeA/marC arabinose efflux transporter, arabinose‑inducible/UPF0056 family inner membrane protein
* ? NC_000913 = 73425050 (1.150)10 (0.240) 6/282 2.6 17.4% pseudogene (118/1521 nt) rhsO pseudogene, Rhs family
?NC_000913 = 3622011 NA (NA)coding (2820/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 = 431852834 (0.780)5 (0.120) 5/280 2.8 13.2% coding (237/759 nt) phnK carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein
?NC_000913 = 4318586 34 (0.830)coding (179/759 nt) phnK carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein
* ? NC_000913 = 3620868NA (NA)5 (0.120) 5/276 2.8 13.1% coding (1677/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3621008 36 (0.830)coding (1817/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 = 354465131 (0.710)5 (0.120) 5/280 2.8 14.5% coding (194/771 nt) bioH pimeloyl‑ACP methyl ester carboxylesterase
?NC_000913 = 3544692 30 (0.730)coding (153/771 nt) bioH pimeloyl‑ACP methyl ester carboxylesterase
* ? NC_000913 = 334029648 (1.100)5 (0.120) 5/280 2.8 9.6% coding (22/978 nt) yrbG putative calcium/sodium:proton antiporter
?NC_000913 = 3340323 49 (1.200)coding (49/978 nt) yrbG putative calcium/sodium:proton antiporter