BRESEQ :: Evidence

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	2601406 =	429 (1.180)	4 (0.010)	5/236	NT	1.1%	intergenic (‑818/+49)	dapF/USA300HOU_RS13395	Diaminopimelate epimerase/hypothetical protein
*	?	USA300TCH1516_ALE	2601436 =	334 (1.010)	4 (0.010)	5/236	NT	1.1%	intergenic (‑848/+19)	dapF/USA300HOU_RS13395	Diaminopimelate epimerase/hypothetical protein
Rejected: Frequency below/above cutoff threshold.

Only 100 of 431 total aligned reads displayed.

TCCTTTTGTTAATCTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTACAAACGTGTTAATCTCTC  >  USA300TCH1516_ALE/2601296‑2601576

 ccTTTTGTTAATCTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTaa                                                                                                                                             <  1:3341130/141‑1 (MQ=255)

  cTTTTGTTAATCTACAATGGATACCCTTGTCTTTCTCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTaaa                                                                                                                                            <  1:1012554/141‑1 (MQ=255)

  cTTTTGTTAATCTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTaaa                                                                                                                                            <  1:3955864/141‑1 (MQ=255)

    tttGTTAATCTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTagaaa                                                                                                                                          >  1:2875731/1‑141 (MQ=255)

       tttAATCTACAATAGATACCCTTGTCTTTCGCTAACATTTCCAATTACCAATACTGTAACGGATTTTCATCGTGAATTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAAttt                                                                                                                                       <  2:1008504/140‑1 (MQ=255)

       gTTAATCTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAAttt                                                                                                                                       >  1:2332381/1‑141 (MQ=255)

        ttAATCTATGATGGAGACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTta                                                                                                                                      <  2:2635520/141‑1 (MQ=255)

        ttAATCTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTta                                                                                                                                      >  1:3375153/1‑141 (MQ=255)

         tAATCTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTtaa                                                                                                                                     >  1:4093007/1‑141 (MQ=255)

          aaTCTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTtaaa                                                                                                                                    <  1:2458375/141‑1 (MQ=255)

            tCTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGTATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAATAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAACAATTcaaata                                                                                                                                  >  1:2648543/1‑141 (MQ=255)

            tCTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTtaaata                                                                                                                                  >  1:4227455/1‑141 (MQ=255)

            tCTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTtaaata                                                                                                                                  >  1:205971/1‑141 (MQ=255)

             cTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTCTTAGCGGGCCTGGAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTtaaataa                                                                                                                                 <  1:1882337/141‑1 (MQ=255)

              tACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTtaaataaa                                                                                                                                <  2:3579042/141‑1 (MQ=255)

               aCAATGTATACCCTTGTCTTTCTGTAACATTTCCTATTTCCAATCCTCTAACGGCTTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAATCTTTGT‑CCAATTTCGTACAC‑AGCTTTAAAAATTTAAATaaaa                                                                                                                               <  1:153886/141‑1 (MQ=255)

               aCAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTATCAATACGGTAACGGATTTTCATCGTCAAGTTGTTGCCCATAAAATACGCAACAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATaaaa                                                                                                                               <  2:4137871/141‑1 (MQ=255)

               aCAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCCTACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTCAATaaaa                                                                                                                               >  2:580707/1‑141 (MQ=255)

               aCAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATaaaa                                                                                                                               >  2:1208910/1‑141 (MQ=255)

                 aaTGGGTACCCTTGTCTTTCGCTAACATTTCCGAGTACCAATCCTGTAGCGGATTTTCATCGTCACGTTGTTGGAAAAGTTTTACCCA‑CAAAACAGAATTCTGACTTTTTCTTACAA‑AGCTTTAAAAATTTAAATAAAAat                                                                                                                             <  1:3251842/141‑1 (MQ=255)

                    ggATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATc                                                                                                                          >  1:517794/1‑141 (MQ=255)

                            ttGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTGTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAAAATCATTGtttt                                                                                                                  >  1:132198/1‑141 (MQ=255)

                            ttGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATATTCATCGACAAGATGTTTCGCATACAATCCGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGtttt                                                                                                                  >  1:1691642/1‑141 (MQ=255)

                             tGTCTTTCGCTAACATTTCCTATTACCGATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTa                                                                                                                 <  1:1807192/141‑1 (MQ=255)

                             tGTCTTTCGCTAACATGTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTACAAATTTAAATAAAAATATCATTGTTTTa                                                                                                                 >  1:2108983/1‑141 (MQ=255)

                              gTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTaa                                                                                                                <  1:2561882/141‑1 (MQ=255)

                              cctgaattacaagtattagcaaacgaaataaaatctgatatggctagttcattaaaacaatgatatttttatttaaatttttaaagctttgtacaatttcttacaaatctttAAAAATTTAAATAAAAATATCATTGtttt                                                                                                                  >  2:105985‑M2/113‑141 (MQ=255)

                               tCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAAt                                                                                                               <  1:3676462/141‑1 (MQ=255)

                               tCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGATTTAAAAATTTAAATAAAAATATCATTGTTTTAAt                                                                                                               <  1:1253054/141‑1 (MQ=255)

                                cTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATg                                                                                                              <  2:4359231/141‑1 (MQ=255)

                                cTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATg                                                                                                              <  1:3591622/141‑1 (MQ=255)

                                   tCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAACAAGCTTTGT‑GCCATTTCGTACAA‑AGCTTTAAAACTTTAAATAAAACTATCATTGTTTTAATGTAc                                                                                                           >  2:222952/1‑141 (MQ=255)

                                   tCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGaaa                                                                                                           >  1:3188592/1‑140 (MQ=255)

                                   tCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAAc                                                                                                           >  1:306673/1‑141 (MQ=255)

                                   tCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAAc                                                                                                           >  1:2915945/1‑141 (MQ=255)

                                   tCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAAc                                                                                                           >  1:1778096/1‑141 (MQ=255)

                                        aaCATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGcc                                                                                                      >  1:3535118/1‑141 (MQ=255)

                                           aTTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCata                                                                                                   <  2:756807/141‑1 (MQ=255)

                                              tCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCa                                                                                                >  1:2231884/1‑141 (MQ=255)

                                                cTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTTTTTTAATGAACTAGCCATATCAGa                                                                                              >  2:316832/1‑141 (MQ=255)

                                                  aTTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGAtt                                                                                            >  2:640218/1‑141 (MQ=255)

                                                    tACCAATCCATAAACTGATTGTCATCGTCAAGTTGTTACAGATATAATACGCACCAAAAAAGGTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGAtttt                                                                                          <  2:943495/141‑1 (MQ=255)

                                                     aCCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTa                                                                                         <  1:3436330/141‑1 (MQ=255)

                                                     aCCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTa                                                                                         <  2:1610469/141‑1 (MQ=255)

                                                      cgaaTCCTGTAACGGATCTTCATCATTAAGTTGTTGTCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTAt                                                                                        <  2:2386126/139‑1 (MQ=255)

                                                      ccAATCCTGTAACGGATTTTGCTCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTAt                                                                                        <  1:3906507/141‑1 (MQ=255)

                                                      ccAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTAt                                                                                        <  1:583272/141‑1 (MQ=255)

                                                       cAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTAtt                                                                                       <  2:218946/141‑1 (MQ=255)

                                                       cAATCCTCTAACGGATTTACATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTAtt                                                                                       <  2:1317343/141‑1 (MQ=255)

                                                           ccTGTAACGGATTTTCATCGTCAATGTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGt                                                                                   <  2:2774251/141‑1 (MQ=255)

                                                           ccTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGt                                                                                   >  2:1636599/1‑141 (MQ=255)

                                                           ccTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGt                                                                                   <  1:892893/141‑1 (MQ=255)

                                                           ccTGTAACGGATTGTTATCGTCAAGTTGTTGCCCATACAATACGCAACAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGt                                                                                   <  2:3915908/141‑1 (MQ=255)

                                                             tGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGttt                                                                                 >  1:3118235/1‑141 (MQ=255)

                                                               tAACTGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTgt                                                                               >  2:1840293/1‑140 (MQ=255)

                                                                aaCGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCt                                                                              <  1:1358128/141‑1 (MQ=255)

                                                                aaCGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTAGTTTGCt                                                                              >  2:2604939/1‑141 (MQ=255)

                                                                aaCGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGATTTAATGAACTAGCCATATCAGGTTTTATTTCGTTTGCt                                                                              >  1:739318/1‑141 (MQ=255)

                                                                      ttttCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTactact                                                                        >  2:3687907/1‑141 (MQ=255)

                                                                      ttttCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACt                                                                        <  2:700985/141‑1 (MQ=255)

                                                                      ttttCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAAAATCATTGTTTTAATGCACTAACCATATCAGAGTACATCTCGTTTGCTAATACt                                                                        >  2:4251210/1‑141 (MQ=255)

                                                                       tttCATCGTCAAGTTGTTGCCCATACACTAAGAACAAAACAAACTTCGA‑ACAA‑TTAGCACATCTACAAAAATAAGTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACtt                                                                       >  1:4395240/1‑141 (MQ=255)

                                                                       tttCATCGTCAAGTTGTTGCCCATACAATGCGCACCAAAAAAGCGTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACtt                                                                       <  2:3329342/141‑1 (MQ=255)

                                                                       tttCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACtt                                                                       >  1:2736212/1‑141 (MQ=255)

                                                                       tttCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACtt                                                                       <  1:4033733/141‑1 (MQ=255)

                                                                        ttCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAGATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTg                                                                      >  1:2362386/1‑141 (MQ=255)

                                                                        ttCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTg                                                                      <  1:3770326/141‑1 (MQ=255)

                                                                         tCATCTTCAAGTTATTGCTCATATAATATGCACAAAAAAAGCTTTGT‑ATAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGt                                                                     <  2:438964/141‑1 (MQ=255)

                                                                         tCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTGATACttat                                                                     >  2:207067/1‑139 (MQ=255)

                                                                         tCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGt                                                                     <  1:1426453/141‑1 (MQ=255)

                                                                          cATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTa                                                                    >  2:2958951/1‑141 (MQ=255)

                                                                             cGTCAAGTTGTTGCCCATACAATACGCATCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAAtt                                                                 <  1:2037394/141‑1 (MQ=255)

                                                                             cGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTGCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAAtt                                                                 <  1:1133266/141‑1 (MQ=255)

                                                                              gTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAGATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTc                                                                <  2:134896/141‑1 (MQ=255)

                                                                                 aaGTTGTAGTCTATACAATAAGCACAAAAAAAGCTTTGT‑ACAATTTAGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAgg                                                             <  2:2844271/141‑1 (MQ=255)

                                                                                   gttgttGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGa                                                           <  2:4196792/141‑1 (MQ=255)

                                                                                    ttgttgCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGaa                                                          >  1:671250/1‑141 (MQ=255)

                                                                                     tgttgCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAg                                                         <  1:3132620/141‑1 (MQ=255)

                                                                                       ttgCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTa                                                       >  2:2269136/1‑141 (MQ=255)

                                                                                                  aTACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCaa                                            >  2:3435936/1‑141 (MQ=255)

                                                                                                  aTACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCaa                                            >  2:1730662/1‑141 (MQ=255)

                                                                                                   tACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTACTACTTGTAATTCAGGTAAGTAATTATTACCaaa                                           >  2:4063591/1‑141 (MQ=255)

                                                                                                         ccAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATAATTGATTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTACTACTTCTAATTCAGGGAAGTAATTATTAGCACAGcatct                                     >  2:3051770/1‑137 (MQ=255)

                                                                                                                 aCTTTGT‑ACAATTTAGTACAA‑AGTTTTAAAAATTTAAATAAAAATATTATTGTTTTAATGAACTAGCCATACCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTAttattt                             >  2:3247253‑M1/2‑141 (MQ=255)

                                                                                                                 GCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTAttattt                             >  1:4288492‑M1/1‑141 (MQ=255)

                                                                                                                  CTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGTAAAGCCAATTAttatttt                            <  2:3532708‑M1/141‑1 (MQ=255)

                                                                                                                     TGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTAttattttatt                         <  2:4301305‑M1/141‑1 (MQ=255)

                                                                                                                         ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAAGGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTAttttt                      >  2:1991226‑M1/1‑141 (MQ=255)

                                                                                                                           aaTTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTa                    <  1:2847199/141‑1 (MQ=255)

                                                                                                                              ttCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTACaa                 <  1:3800815/141‑1 (MQ=255)

                                                                                                                               tCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTACaaa                <  1:3827814/141‑1 (MQ=255)

                                                                                                                                cGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTACaaat               >  2:3815671/1‑140 (MQ=255)

                                                                                                                                cGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTACAAAc               >  1:1647699/1‑141 (MQ=255)

                                                                                                                                cGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTACAAAc               >  2:3748764/1‑141 (MQ=255)

                                                                                                                                  gtacaaagctttAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGTGAAGTAATTATTACCAAAGCCAATTATTATTTTATTTTTTACAATCg              >  2:1897557‑M2/13‑141 (MQ=255)

                                                                                                                                  gtacaaagctttAAAAATTTAAATAAAAATAGCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTACAAACg              <  2:397417‑M2/129‑1 (MQ=255)

                                                                                                                                    acaaagctttAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTACAAACgtg            <  1:300657‑M2/131‑1 (MQ=255)

                                                                                                                                    acaaagctttAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTACAAACgtg            >  1:1631648‑M2/11‑141 (MQ=255)

                                                                                                                                      aaagctttAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTACAAACGTGtt          >  2:141690‑M2/9‑141 (MQ=255)

                                                                                                                                            ttAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTACAAACTTGTTAAtctc    >  2:2487578‑M2/3‑141 (MQ=255)

TCCTTTTGTTAATCTACAATGGATACCCTTGTCTTTCGCTAACATTTCCTATTACCAATCCTGTAACGGATTTTCATCGTCAAGTTGTTGCCCATACAATACGCACCAAAAAAGCTTTGT‑ACAATTTCGTACAA‑AGCTTTAAAAATTTAAATAAAAATATCATTGTTTTAATGAACTAGCCATATCAGATTTTATTTCGTTTGCTAATACTTGTAATTCAGGGAAGTAATTATTAGCAAAGCCAATTATTATTTTATTTTTTACAAACGTGTTAATCTCTC  >  USA300TCH1516_ALE/2601296‑2601576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.