BRESEQ :: Evidence

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 2681424	107 (1.360)	3 (0.040)	3/250	8.8	3.0%	intergenic (‑459/+120)	USA300HOU_RS13810/mvaA	hypothetical protein/3‑hydroxy‑3‑methylglutaryl‑coenzyme A reductase
*	?	USA300TCH1516_ALE	= 2681449	98 (1.400)	3 (0.040)	3/250	8.8	3.0%	intergenic (‑484/+95)	USA300HOU_RS13810/mvaA	hypothetical protein/3‑hydroxy‑3‑methylglutaryl‑coenzyme A reductase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 108 total aligned reads displayed.

GGAACTTGAAGAATCTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTATTGTTGTCTAATTTC  >  USA300TCH1516_ALE/2681285‑2681561

ggtaCTTGAAGAATCTCAGACCGTTAAAATCGAGACAATTAGAAACTCGGTCTAAATTTGAATCCTATCATGAATGTGCCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACg                                                                                                                                          <  2:456562/138‑1 (MQ=255)

ggAACTTGAAGAATCTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATATACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACg                                                                                                                                          >  2:782751/1‑141 (MQ=255)

     ttGAAGAATCTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGc                                                                                                                                     >  1:847921/1‑141 (MQ=255)

     ttGAAGAATCTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAAAGc                                                                                                                                     >  1:249439/1‑141 (MQ=255)

      tcaagaaTCTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCt                                                                                                                                    <  2:228307/139‑1 (MQ=255)

      tGAAGAATCTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCt                                                                                                                                    <  1:684329/141‑1 (MQ=255)

        aaggaTCTAAGCCTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCAGGAAGAAGTAATTTACGTTTCATTAGACTGCGTTACTAATCAAAAAACTTCTATCACGAACGCTTc                                                                                                                                  >  2:295364/1‑141 (MQ=39)

        aagaaTCTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAGTTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTc                                                                                                                                  >  2:237621/1‑141 (MQ=255)

        aagaaTCTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTc                                                                                                                                  <  2:664259/141‑1 (MQ=255)

         agaaTCTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCa                                                                                                                                 >  1:545130/1‑141 (MQ=255)

         agaaTCTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCa                                                                                                                                 >  1:39839/1‑141 (MQ=255)

          gaaTCTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCaa                                                                                                                                >  1:377954/1‑141 (MQ=255)

              cTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTc                                                                                                                            >  1:407599/1‑141 (MQ=255)

                aaGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCt                                                                                                                          >  2:862467/1‑141 (MQ=255)

                   aCTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATACCGCCCCCTCCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTa                                                                                                                       <  1:669697/141‑1 (MQ=37)

                        tAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCgt                                                                                                                  <  1:239638/141‑1 (MQ=255)

                             tCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGAt                                                                                                             <  1:343779/141‑1 (MQ=255)

                              cGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATa                                                                                                            >  2:849277/1‑141 (MQ=255)

                                   cAATAAGAAACTCGTTCCAAACACGAATCCTATAATGAATTCGTCTCATGACGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGt                                                                                                       >  2:654691/1‑141 (MQ=255)

                                       aaGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGttttt                                                                                                   <  2:899937/141‑1 (MQ=255)

                                        aGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATCAAAGTTTTTa                                                                                                  <  1:676367/141‑1 (MQ=255)

                                        aGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTa                                                                                                  <  2:267747/141‑1 (MQ=255)

                                        aGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTa                                                                                                  <  1:29112/141‑1 (MQ=255)

                                        aGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTa                                                                                                  <  2:899689/141‑1 (MQ=255)

                                          aaaCTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTAtt                                                                                                <  2:34261/141‑1 (MQ=255)

                                           aaCTCGATCCAAATTTAAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTAttt                                                                                               <  2:504780/141‑1 (MQ=255)

                                            aCTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTAtttt                                                                                              <  2:134122/141‑1 (MQ=255)

                                            aCTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTAtttt                                                                                              <  1:694999/141‑1 (MQ=255)

                                            aCTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTAtttt                                                                                              <  1:890836/141‑1 (MQ=255)

                                             cTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTGATCAAAAAACTTCTATCACGAAAGATTCAATTTCCTGTTTGTGTGGGGTAGAAGgtttttggtta                                                                                             >  1:871631/1‑135 (MQ=37)

                                               agTTCCAAATTTGATACCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTgc                                                                                           <  2:489430/140‑2 (MQ=255)

                                                  tCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCa                                                                                        >  2:161387/1‑141 (MQ=255)

                                                  tCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACCTCAGATTTTACTGCATTGCCAATCAAAAAACTTCTATCACGGACGCGGCAATTTCCTGTAGGCGTGTGAGAGAAGTTTTTATTTTTGTTCa                                                                                        >  1:216502/1‑141 (MQ=21)

                                                    cAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCata                                                                                      <  1:279243/141‑1 (MQ=255)

                                                     aaaTTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCatat                                                                                     <  1:38321/141‑1 (MQ=255)

                                                     aaaTTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCatat                                                                                     <  2:778661/141‑1 (MQ=255)

                                                     aaaTTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCatat                                                                                     <  2:778666/141‑1 (MQ=255)

                                                     aaaTTTGAATCCTATAAGGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCatat                                                                                     <  2:541760/141‑1 (MQ=255)

                                                      aaTTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATAtt                                                                                    >  1:412649/1‑141 (MQ=255)

                                                      aaTTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATAtt                                                                                    <  1:226966/141‑1 (MQ=255)

                                                        tttGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTaa                                                                                  <  1:495190/141‑1 (MQ=255)

                                                          tGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACGTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAAtt                                                                                >  1:631850/1‑141 (MQ=255)

                                                                    aaTGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAgcg                                                                      >  2:234265/1‑141 (MQ=255)

                                                                          ttCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGCGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGa                                                                <  2:732822/141‑1 (MQ=255)

                                                                          ttCGTCTCATGAAGAAGTTATTTACTTTTCATGAGACGGAGTTACTAATCAAAACACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGa                                                                >  1:255957/1‑141 (MQ=255)

                                                                             gTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTTTTCTTATTAATTTATCTAAGCGCTACGATgg                                                             >  2:622670/1‑141 (MQ=255)

                                                                             gTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATgg                                                             >  1:707367/1‑141 (MQ=255)

                                                                               ctcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgac                                                           <  2:356751/141‑1 (MQ=255)

                                                                               cgcATGAAGAAGTTATTTAGTTGGCATTAGAGTGTTTTCCTAAACAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTAGGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgac                                                           <  2:783948/139‑1 (MQ=21)

                                                                                tcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgact                                                          >  1:719332/1‑141 (MQ=255)

                                                                                tcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgact                                                          >  2:292886/1‑141 (MQ=255)

                                                                                tcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgact                                                          >  1:925077/1‑141 (MQ=255)

                                                                                tcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgact                                                          >  2:167656/1‑141 (MQ=255)

                                                                                tcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgact                                                          >  1:292357/1‑141 (MQ=255)

                                                                                tcATGAAGAAGTTATTTACGTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTGTGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgact                                                          >  1:489198/1‑141 (MQ=255)

                                                                                    gaagaaGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTCCGATGgactgact                                                      >  2:330776/1‑141 (MQ=255)

                                                                                      agaagTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAATTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTa                                                    >  1:255386/1‑141 (MQ=255)

                                                                                       gaagTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTaa                                                   <  1:630045/141‑1 (MQ=255)

                                                                                       gaagTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTGCAATTTCCTGTCTGCGTGGGATAGGAGGCTCGATTATTTTTCATATTAATTTCTCTAAGGGCTACGCTGGGCTGGCTTaa                                                   >  1:398947/1‑141 (MQ=21)

                                                                                         agTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAAc                                                 <  2:850904/141‑1 (MQ=255)

                                                                                             aTTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCTCGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACtttt                                             >  1:868756/1‑141 (MQ=255)

                                                                                             aTTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACtttt                                             >  1:186231/1‑141 (MQ=255)

                                                                                                tACTTTTCATTAGACTGATTTACTAATGAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCtt                                          <  2:479493/141‑1 (MQ=255)

                                                                                                tACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCtt                                          <  1:61093/141‑1 (MQ=255)

                                                                                                tACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTACCACGAACGTTTCAATTTCCTTTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATAATTTTATCCAAGCCCTACGATGGACTGACTAAAACTTTTCtt                                          <  2:526900/141‑1 (MQ=25)

                                                                                                tACTTTTCATTAGACTGATATACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCtt                                          >  1:550766/1‑141 (MQ=255)

                                                                                                        aTTAGACTTATTTACTAAGTAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCg                                  <  2:832340/141‑1 (MQ=255)

                                                                                                        aTTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGCGTGACTGAAAAAAACATTTCTTTAAAATCg                                  <  2:609742/141‑1 (MQ=21)

                                                                                                         ttAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGc                                 <  1:849277/141‑1 (MQ=255)

                                                                                                           aGACTGATTTACTAATCAAAAAACTTCTATCACGACAGCTTCAATTTCCTGTATGCGTGTGATAGAAGGTTTTGTTTTTGTTCATATTAATTTATCTAAACGCTACGCTGGTCTGACTTAAACTTTTCTTTAACATCGCTa                               >  2:278583/1‑141 (MQ=25)

                                                                                                           aGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCtt                               >  1:41804/1‑140 (MQ=255)

                                                                                                           aGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTa                               <  1:234265/141‑1 (MQ=255)

                                                                                                           aGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTATATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCtt                               >  1:610462/1‑140 (MQ=255)

                                                                                                                    tACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCAtt                      >  2:396275/1‑141 (MQ=255)

                                                                                                                     aCTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCAttt                     >  1:859503/1‑141 (MQ=255)

                                                                                                                       tAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTAc                   <  1:782751/141‑1 (MQ=255)

                                                                                                                        aaTCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACt                  >  1:120601/1‑141 (MQ=255)

                                                                                                                         aTCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTa                 >  2:440554/1‑141 (MQ=255)

                                                                                                                          taaaaaaaCTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTAt                <  2:704082/139‑1 (MQ=255)

                                                                                                                           cAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTACAATCGCTCTTCGCCATTTCCTAtt               >  2:619229/1‑141 (MQ=255)

                                                                                                                           cAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTAtt               >  1:609870/1‑141 (MQ=255)

                                                                                                                           cAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTAtt               >  1:438780/1‑141 (MQ=255)

                                                                                                                            aaaaaaATTCTATCACGAACGATTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTAGCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTAttg              <  1:361295/141‑1 (MQ=255)

                                                                                                                              aaaaCTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGCCTGACTTAAACTTTTCTTTAAAATAGCTATTCGCCATTTCCTAttgtt            >  1:171019/1‑141 (MQ=255)

                                                                                                                               aaaCTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTGttgttg           >  1:68321/1‑141 (MQ=255)

                                                                                                                               aaaCTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTAttgttg           <  2:39839/141‑1 (MQ=255)

                                                                                                                               aaaCTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGAGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCACTATTCGCCATTTACTAttgttt           >  2:122801/1‑140 (MQ=255)

                                                                                                                                  cTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTATTGTTGTCt        >  2:11747/1‑141 (MQ=255)

                                                                                                                                   ttCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTATTGTTGTCTa       >  2:821593/1‑141 (MQ=255)

                                                                                                                                   ttCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAACGCTACGATGGACTGACTTAAACTTTTCTTTAAAATAGCTATTCGCCATTTACTCTTGTTGTCtt       >  2:668968/1‑140 (MQ=255)

                                                                                                                                   tccTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTATTGTTGTCTa       >  2:465148/3‑141 (MQ=255)

                                                                                                                                    tCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTATTGTTGTCTaa      <  2:545130/141‑1 (MQ=255)

                                                                                                                                     cTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTCTTCGCCATTTACTATTGTTGTCTAAt     >  1:459217/1‑141 (MQ=255)

                                                                                                                                     cTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTATTGTTGTCTAAt     >  1:757049/1‑141 (MQ=255)

                                                                                                                                     cTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTGGGTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTATTGTTGTCTAAt     >  2:692863/1‑141 (MQ=255)

                                                                                                                                      tATCACGAACGCTTCAATTTCCTGTATGCGTGTGATCGCAGTTTTTATTTTTGTTGATATTAATTTATCTAAGCGATACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTCTTCGCCCTTTACTATGTTTGTCTaact    >  2:581255/1‑139 (MQ=37)

                                                                                                                                      tATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTATTGTTGTCTAAtt    >  2:887865/1‑141 (MQ=255)

                                                                                                                                      tATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTATTGTTGTCTAAtt    >  1:654427/1‑141 (MQ=255)

                                                                                                                                        tCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTATTGTTGTCTAATTTc  <  1:440136/141‑1 (MQ=255)

                                                                                                                                        tCACGAACGCTTCAATTTCCGGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTATTGTTGTCTAATTTc  <  1:440164/141‑1 (MQ=255)

GGAACTTGAAGAATCTAAGACTGTTAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCTATTCGCCATTTACTATTGTTGTCTAATTTC  >  USA300TCH1516_ALE/2681285‑2681561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.