BRESEQ :: Evidence

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 2681424	107 (1.360)	3 (0.040)	3/250	8.8	3.0%	intergenic (‑459/+120)	USA300HOU_RS13810/mvaA	hypothetical protein/3‑hydroxy‑3‑methylglutaryl‑coenzyme A reductase
*	?	USA300TCH1516_ALE	= 2681449	98 (1.400)	3 (0.040)	3/250	8.8	3.0%	intergenic (‑484/+95)	USA300HOU_RS13810/mvaA	hypothetical protein/3‑hydroxy‑3‑methylglutaryl‑coenzyme A reductase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 105 total aligned reads displayed.

TAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAATTTCTTGTAAAATATGTTCCGCTGCTTGTG  >  USA300TCH1516_ALE/2681309‑2681587

tAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCgt                                                                                                                                             <  1:239638/141‑1 (MQ=255)

     tCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGAt                                                                                                                                        <  1:343779/141‑1 (MQ=255)

      cGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATa                                                                                                                                       >  2:849277/1‑141 (MQ=255)

           cAATAAGAAACTCGTTCCAAACACGAATCCTATAATGAATTCGTCTCATGACGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGt                                                                                                                                  >  2:654691/1‑141 (MQ=255)

              tAAGAAACTCGTTCCAAATTTGAATCCTATAAAAAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGtttt                                                                                                                               >  1:445015/1‑141 (MQ=255)

               aaGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGttttt                                                                                                                              <  2:899937/141‑1 (MQ=255)

                aGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATCCAAGTTTTTa                                                                                                                             <  1:676424/141‑1 (MQ=255)

                aGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATCAAAGTTTTTa                                                                                                                             <  1:676367/141‑1 (MQ=255)

                aGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTa                                                                                                                             <  2:267747/141‑1 (MQ=255)

                aGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTa                                                                                                                             <  2:899689/141‑1 (MQ=255)

                aGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTa                                                                                                                             <  1:29112/141‑1 (MQ=255)

                 gAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTAt                                                                                                                            <  1:316005/141‑1 (MQ=255)

                  aaaCTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTAtt                                                                                                                           <  2:34261/141‑1 (MQ=255)

                   aaCTCGATCCAAATTTAAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTAttt                                                                                                                          <  2:504780/141‑1 (MQ=255)

                    aCTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTAtttt                                                                                                                         <  2:134122/141‑1 (MQ=255)

                    aCTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTAtttt                                                                                                                         <  1:890836/141‑1 (MQ=255)

                    aCTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTAtttt                                                                                                                         <  1:694999/141‑1 (MQ=255)

                     cTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTGATCAAAAAACTTCTATCACGAAAGATTCAATTTCCTGTTTGTGTGGGGTAGAAGgtttttggtta                                                                                                                        >  1:871631/1‑135 (MQ=37)

                       agTTCCAAATTTGATACCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTgc                                                                                                                      <  2:489430/140‑2 (MQ=255)

                          tCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCa                                                                                                                   >  2:161387/1‑141 (MQ=255)

                          tCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACCTCAGATTTTACTGCATTGCCAATCAAAAAACTTCTATCACGGACGCGGCAATTTCCTGTAGGCGTGTGAGAGAAGTTTTTATTTTTGTTCa                                                                                                                   >  1:216502/1‑141 (MQ=21)

                          gccAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTATATTTTTGTTCa                                                                                                                   >  2:516114/2‑141 (MQ=255)

                            cAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCata                                                                                                                 <  1:279243/141‑1 (MQ=255)

                             aaaTTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCatat                                                                                                                <  2:778661/141‑1 (MQ=255)

                             aaaTTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCatat                                                                                                                <  2:778666/141‑1 (MQ=255)

                             aaaTTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCatat                                                                                                                <  1:38321/141‑1 (MQ=255)

                             aaaTTTGAATCCTATAAGGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCatat                                                                                                                <  2:541760/141‑1 (MQ=255)

                              aaTTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATAtt                                                                                                               >  1:412649/1‑141 (MQ=255)

                              aaTTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATAtt                                                                                                               <  1:226966/141‑1 (MQ=255)

                                tttGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTaa                                                                                                             <  1:495190/141‑1 (MQ=255)

                                  tGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACGTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAAtt                                                                                                           >  1:631850/1‑141 (MQ=255)

                                            aaTGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAgcg                                                                                                 >  2:234265/1‑141 (MQ=255)

                                                  ttCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGCGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGa                                                                                           <  2:732822/141‑1 (MQ=255)

                                                  ttCGTCTCATGAAGAAGTTATTTACTTTTCATGAGACGGAGTTACTAATCAAAACACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGa                                                                                           >  1:255957/1‑141 (MQ=255)

                                                     gTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATgg                                                                                        >  1:707367/1‑141 (MQ=255)

                                                       ctcATGAAGAAGTTATTTACTTTTCATTAGACTGCTTTACTAATAAAAAAACTTCTATGAAGAACGCTTCAATTTCCTATATGAGTGTGATGGAAATTTTTAGTATTGTTCATCTTACTTTATAAAAGCGCTAcactggaa                                                                                      >  1:881830/1‑134 (MQ=255)

                                                       ctcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgac                                                                                      <  2:356751/141‑1 (MQ=255)

                                                       cgcATGAAGAAGTTATTTAGTTGGCATTAGAGTGTTTTCCTAAACAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTAGGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgac                                                                                      <  2:783948/139‑1 (MQ=21)

                                                        tcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTTTTCATATTAATTTATCTAAGCGCTACGATGggct                                                                                     >  2:293712/1‑141 (MQ=255)

                                                        tcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgact                                                                                     >  1:925077/1‑141 (MQ=255)

                                                        tcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgact                                                                                     >  2:292886/1‑141 (MQ=255)

                                                        tcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgact                                                                                     >  2:167656/1‑141 (MQ=255)

                                                        tcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgact                                                                                     >  1:292357/1‑141 (MQ=255)

                                                        tcATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgact                                                                                     >  1:719332/1‑141 (MQ=255)

                                                        tcATGAAGAAGTTATTTACGTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTGTGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGgact                                                                                     >  1:489198/1‑141 (MQ=255)

                                                              agaagTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAATTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTa                                                                               >  1:255386/1‑141 (MQ=255)

                                                               gaagTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTaa                                                                              <  1:630045/141‑1 (MQ=255)

                                                               gaagTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTGCAATTTCCTGTCTGCGTGGGATAGGAGGCTCGATTATTTTTCATATTAATTTCTCTAAGGGCTACGCTGGGCTGGCTTaa                                                                              >  1:398947/1‑141 (MQ=21)

                                                                 agTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAAc                                                                            <  2:850904/141‑1 (MQ=255)

                                                                     aTTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCTCGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACtttt                                                                        >  1:868756/1‑141 (MQ=255)

                                                                     aTTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACtttt                                                                        >  1:186231/1‑141 (MQ=255)

                                                                        tACTTTTCATTAGACTGATTTACTAATGAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCtt                                                                     <  2:479493/141‑1 (MQ=255)

                                                                        tACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCtt                                                                     <  1:61093/141‑1 (MQ=255)

                                                                        tACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTACCACGAACGTTTCAATTTCCTTTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATAATTTTATCCAAGCCCTACGATGGACTGACTAAAACTTTTCtt                                                                     <  2:526900/141‑1 (MQ=25)

                                                                        tACTTTTCATTAGACTGATATACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCtt                                                                     >  1:550766/1‑141 (MQ=255)

                                                                                aTTAGACTTATTTACTAAGTAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCg                                                             <  2:832340/141‑1 (MQ=255)

                                                                                aTTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGCGTGACTGAAAAAAACATTTCTTTAAAATCg                                                             <  2:609742/141‑1 (MQ=21)

                                                                                 ttAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGc                                                            <  1:849277/141‑1 (MQ=255)

                                                                                   aGACTGATTTACTAATCAAAAAACTTCTATCACGACAGCTTCAATTTCCTGTATGCGTGTGATAGAAGGTTTTGTTTTTGTTCATATTAATTTATCTAAACGCTACGCTGGTCTGACTTAAACTTTTCTTTAACATCGCT‑a                                                         >  2:278583/1‑141 (MQ=25)

                                                                                   aGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCtt                                                          >  1:41804/1‑140 (MQ=255)

                                                                                   aGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑a                                                         <  1:234265/141‑1 (MQ=255)

                                                                                   aGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTATATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCtt                                                          >  1:610462/1‑140 (MQ=255)

                                                                                            tACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCAtt                                                >  2:396275/1‑141 (MQ=255)

                                                                                            tACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGCGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCCtt                                                >  2:748913/1‑141 (MQ=255)

                                                                                             aCTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCAttt                                               >  1:859503/1‑141 (MQ=255)

                                                                                               tAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTAc                                             <  1:782751/141‑1 (MQ=255)

                                                                                                aaTCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACt                                            >  1:120601/1‑141 (MQ=255)

                                                                                                 aTCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTa                                           >  2:440554/1‑141 (MQ=255)

                                                                                                  taaaaaaaCTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTAt                                          <  2:704082/139‑1 (MQ=255)

                                                                                                   cAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTACAATCGCT‑CTTCGCCATTTCCTAtt                                         >  2:619229/1‑141 (MQ=255)

                                                                                                   cAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTAtt                                         >  1:609870/1‑141 (MQ=255)

                                                                                                    aaaaaaATTCTATCACGAACGATTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTAGCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTAttg                                        <  1:361295/141‑1 (MQ=255)

                                                                                                      aaaaCTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGCCTGACTTAAACTTTTCTTTAAAATAGCT‑ATTCGCCATTTCCTAttgtt                                      >  1:171019/1‑141 (MQ=255)

                                                                                                       aaaCTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTGttgttg                                     >  1:68321/1‑141 (MQ=255)

                                                                                                       aaaCTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTAttgttg                                     <  2:39839/141‑1 (MQ=255)

                                                                                                       aaaCTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGAGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCACT‑ATTCGCCATTTACTAttgttt                                     >  2:122801/1‑140 (MQ=255)

                                                                                                          cTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCt                                  >  2:11747/1‑141 (MQ=255)

                                                                                                           ttCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTa                                 >  2:821593/1‑141 (MQ=255)

                                                                                                           ttCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAACGCTACGATGGACTGACTTAAACTTTTCTTTAAAATAGCT‑ATTCGCCATTTACTCTTGTTGTCtt                                 >  2:668968/1‑140 (MQ=255)

                                                                                                           tccTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTa                                 >  2:465148/3‑141 (MQ=255)

                                                                                                            tCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTaa                                <  2:545130/141‑1 (MQ=255)

                                                                                                             cTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑CTTCGCCATTTACTATTGTTGTCTAAt                               >  1:459217/1‑141 (MQ=255)

                                                                                                             cTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTGGGTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAAt                               >  2:692863/1‑141 (MQ=255)

                                                                                                              tATCACGAACGCTTCAATTTCCTGTATGCGTGTGATCGCAGTTTTTATTTTTGTTGATATTAATTTATCTAAGCGATACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑CTTCGCCCTTTACTATGTTTGTCTaact                              >  2:581255/1‑139 (MQ=37)

                                                                                                              tATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAAtt                              >  2:887865/1‑141 (MQ=255)

                                                                                                              tATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAAtt                              >  1:654427/1‑141 (MQ=255)

                                                                                                                tCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAATTTc                            <  1:440136/141‑1 (MQ=255)

                                                                                                                tCACGAACGCTTCAATTTCCGGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAATTTc                            <  1:440164/141‑1 (MQ=255)

                                                                                                                     aaCGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTGTAATTTCTTGTa                       <  2:407599/141‑1 (MQ=255)

                                                                                                                        gCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAATTTCTTGTaaaa                    <  1:482665/141‑1 (MQ=255)

                                                                                                                          ttCAATTTCCTGTATGCGTGTTAGAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAATTTCTTGTAAAata                  <  2:643899/141‑1 (MQ=255)

                                                                                                                          ttCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTCTTGTTGTCTAATTTCTTGTAAAata                  >  2:346859/1‑141 (MQ=255)

                                                                                                                          ttCAATTTCCGTTTTTCGTTTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAATCTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAATTTCTTGTAAAata                  <  2:428814/141‑1 (MQ=255)

                                                                                                                            cAATCTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACGTTTGTTGTCTAATTTCTTGTAAAATATg                <  1:219284/141‑1 (MQ=255)

                                                                                                                              aTTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAATTTCTTGTAAAATATGtt              <  2:120601/141‑1 (MQ=255)

                                                                                                                              aTTCCCTGTATGCGTGTGCTAGAAGTATTTCTTTTTGTTCATATTAATTTATCAAAGCGCTACGATGAACTGACTTAACATTTCCCTTCAAAT‑GCTAATCCGTACTTTACTATTGTTGTCTCATTACTAGTAACatctcta              >  2:33614/1‑136 (MQ=255)

                                                                                                                                        tgggtggGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAATTTCTTGTAAAATATGTTCCGCTGCTtg    <  2:175836/138‑1 (MQ=255)

                                                                                                                                        tGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAGAATCGCT‑ATTCGCCATTTACTATTGTTGTTTAATTTCTTGTAAAATTGGTTCCGCTGCttt    >  2:805521/1‑140 (MQ=255)

                                                                                                                                        tGCGTGTGATAGAAGTTTTTATTTTTGTACATATTAATTTATCTAAGCGCTACTATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAATTTCTTGTAAAATATGTTCCGCTGCTtg    <  2:837190/141‑1 (MQ=255)

                                                                                                                                          cGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAATTTCTTGTAAAATATGTTCCGCTGCTtgtg  >  2:795475/1‑141 (MQ=255)

TAAAATCGAGACAATAAGAAACTCGTTCCAAATTTGAATCCTATAATGAATTCGTCTCATGAAGAAGTTATTTACTTTTCATTAGACTGATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTTCCTGTATGCGTGTGATAGAAGTTTTTATTTTTGTTCATATTAATTTATCTAAGCGCTACGATGGACTGACTTAAACTTTTCTTTAAAATCGCT‑ATTCGCCATTTACTATTGTTGTCTAATTTCTTGTAAAATATGTTCCGCTGCTTGTG  >  USA300TCH1516_ALE/2681309‑2681587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.