New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 1262909	79 (0.890)	3 (0.040)	3/258	11.0	3.8%	intergenic (+34/‑193)	sucD/lytN_1	Succinate‑‑CoA ligase [ADP‑forming] subunit alpha/putative cell wall hydrolase LytN
	?	USA300TCH1516_ALE	= 1262932	80 (0.970)					intergenic (+57/‑170)	sucD/lytN_1	Succinate‑‑CoA ligase [ADP‑forming] subunit alpha/putative cell wall hydrolase LytN
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CACCTTCAGAAATTGGTTCAACATTAATTGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACTATGTTATAA  >  USA300TCH1516_ALE/1262793‑1263069                                                                                                                                            |                                                                                                                                          cacCTTCAGAAATTGGTTCAACATTAATTGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGa                                                                                                                                           <  2:109938/140‑1 (MQ=255)  acCTTCAGAAATTGGTTCAACATTAAGTGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGaa                                                                                                                                          <  1:447525/140‑1 (MQ=255)      tCAGAAATTTGTTCAACATTAATTGAAGCTGCTAAAGAAGCAGGTATTTAAGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATgg                                                                                                                                      <  1:354498/140‑1 (MQ=255)       cAGAAATTGGTTCAACATTAATTGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATggg                                                                                                                                     <  2:577325/140‑1 (MQ=255)        cgAAATTGGTTTAACATTAATTGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGc                                                                                                                                    <  1:438234/139‑1 (MQ=255)           aaTTGGTTCAACATTAATTGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAATTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATTAAAGCATCGGAAATGGGCTAt                                                                                                                                 <  1:516792/140‑1 (MQ=255)           aaTTGGTTCAACATTAATTGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTAt                                                                                                                                 <  2:666947/140‑1 (MQ=255)           aaTTGGTTCAACATTAATTGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATACCATACTTTATAAAAGTATCGGAAATGGGCTAt                                                                                                                                 <  2:664279/140‑1 (MQ=255)              tGGTTCAACATTAATTGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTg                                                                                                                              <  2:848506/140‑1 (MQ=255)                      cATTAATTGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGa                                                                                                                      <  1:95000/140‑1 (MQ=255)                            ttGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAAtt                                                                                                                >  2:390623/1‑140 (MQ=255)                            ttGAAGCTGCTAAAGAAGCACGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGGGCGATGAAtt                                                                                                                <  2:243749/140‑1 (MQ=255)                                     ctAAAGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCAATACTTTATAAAAGTATCGGAAATGGGCTATTTCTCGTGCGATGGATTTTAGttaac                                                                                                       >  1:829677/1‑136 (MQ=255)                                       aaaGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACtt                                                                                                     <  2:354928/140‑1 (MQ=255)                                               aGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATATCCCATTCCATACTTTATAAAAGTATCGGAACTCGGCTAATTCTTATTCGATGAATTTTAGTACAATTTTCATAtt                                                                                             >  2:454571/1‑140 (MQ=255)                                               aGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATAtt                                                                                             >  1:351735/1‑140 (MQ=255)                                               aGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATAtt                                                                                             >  1:388203/1‑140 (MQ=255)                                                 gTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTTACTAAAATGGGCTATTTTTCGTGCGATGAATTTTAGTACACTTTTCATAtttt                                                                                           >  1:188892/1‑140 (MQ=255)                                                  tATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTg                                                                                          >  1:760922/1‑140 (MQ=255)                                                   aTTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTgg                                                                                         >  1:988778/1‑140 (MQ=255)                                                   aTTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTgg                                                                                         <  2:938212/140‑1 (MQ=255)                                                      tATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACCCTTTTCATATCTTGGaca                                                                                      >  2:936390/1‑138 (MQ=255)                                                       aTGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGGAAg                                                                                     >  1:107159/1‑140 (MQ=255)                                                       aTGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGAGACAAATCGTAGTACACTTTTCATATTTTGGAAAg                                                                                     <  1:738332/140‑1 (MQ=255)                                                          aaactatatgaACTCTTAACAAATAAAATTAAAAGATGATATAAATGGCTATTTCCCATTCCATATTTTATAAAAGTATCGGATATTGGCTATTTGTCGTGAGATGATTTTTAGTACACTTTTCATATTTTGGAAAGACa                                                                                  <  2:8385/134‑1 (MQ=255)                                                          aaTCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGGTGGATTTTAGTACTCTTTTGATATTTTGGAAAGGCa                                                                                  >  1:17092/1‑140 (MQ=255)                                                          aaTCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACa                                                                                  <  2:359156/140‑1 (MQ=255)                                                             aagtattaACTGTTAATAAATAAAGTTAAAAGACGATATAAATGGCTATAGCCCAATCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTTGTGCGATGAATTTTAGTTCACTTTTCATATTTTGGAAAGACAAGt                                                                               <  1:349894/137‑1 (MQ=255)                                                               ttattaACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGACTTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTa                                                                             >  2:527623/1‑140 (MQ=255)                                                                   taACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAatta                                                                         >  1:969588/1‑140 (MQ=255)                                                                   taACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAatta                                                                         >  1:181609/1‑140 (MQ=255)                                                                   taACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAatta                                                                         >  1:787633/1‑140 (MQ=255)                                                                    aaCTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAattat                                                                        <  2:297498/140‑1 (MQ=255)                                                                     aCTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAattatt                                                                       >  1:585477/1‑140 (MQ=255)                                                                     aCTGTTAATAAATAAAGTTAAAAGATAATATAAATGGCTATAGCCCATTCCATAATTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAattatt                                                                       <  2:208685/140‑1 (MQ=255)                                                                       tGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTAtttt                                                                     >  2:849577/1‑140 (MQ=255)                                                                        gTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGACAGACAAGTTAATTATTTTa                                                                    >  2:768947/1‑140 (MQ=255)                                                                             taaataaaGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGTATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTAcctcc                                                               >  2:857856/1‑140 (MQ=255)                                                                              aaataaaGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTCCATATTTTGGAAAGACAGGTTAATTATTTTAcctcct                                                              >  1:256168/1‑140 (MQ=255)                                                                               aataaaGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTAGCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACATTTCATATTTTGGACAGACAAGTTAATTATTTTACCTCCTa                                                             >  2:295987/1‑140 (MQ=255)                                                                                ataaaGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTAc                                                            >  1:658387/1‑140 (MQ=255)                                                                                 taaaGTTAAAAGGTGGTATAAATGGCTATGGCCCATTCCATTCTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACa                                                           <  2:89097/140‑1 (MQ=255)                                                                                      gtAAAAGATGATATAAATGGCTATAGCACATTCCACACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGc                                                      <  2:703147/139‑1 (MQ=255)                                                                                        aaaaGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTGTCGGAAATGGCCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACaccacata                                                    >  2:286241/1‑133 (MQ=255)                                                                                         aaaGATGATCTAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGAAAATGGCCCAATGGTCGTGCGATGAATCTAAGTACACTTTTCACATTTTGCGAAGCCAAGTTAATTATTTTACTTCCTACAATAGCttc                                                   >  2:302391/1‑139 (MQ=255)                                                                                         aaaGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCttt                                                   >  2:304309/1‑140 (MQ=255)                                                                                         aaaGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCttt                                                   >  1:877942/1‑140 (MQ=255)                                                                                         aaaGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTACTACAATAGCttt                                                   >  2:966351/1‑140 (MQ=255)                                                                                           aGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTatat                                                 >  2:422123/1‑140 (MQ=255)                                                                                            gatgatATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTACTTATTTTACCTCCTACAATAGCTTtata                                                >  2:360603/1‑140 (MQ=255)                                                                                            gatgatATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTtata                                                >  2:733037/1‑140 (MQ=255)                                                                                            gatgatATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATAGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACATACTACTATAGCTTtata                                                >  2:683544/1‑140 (MQ=255)                                                                                            gatgatATAAATGGCTATAGCCCATTCCATACTGTATAAAAGTATCGGAAATGGGCTATGTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTACTACAATAGCTTtatg                                                >  2:884396/1‑139 (MQ=255)                                                                                              tgatATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACTTCCTACAATAGCTTTATaaa                                              >  1:653323/1‑140 (MQ=255)                                                                                               gatATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTCCAATATCTTTATaaaa                                             >  1:375594/1‑140 (MQ=255)                                                                                                 tataAATGGCTATAGTCGTGTCCCTACGTTAGAAAAGTTTCGGAAATGGGCCATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACa                                           <  2:999075/140‑1 (MQ=255)                                                                                                 tataAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGTCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTCATTATTTTGACTCCTACAATAGCTTTATAAAAcc                                           >  1:675918/1‑139 (MQ=255)                                                                                                 tataAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTCCATCCTACACTTGCTTTATaaacaa                                           >  2:976954/1‑137 (MQ=255)                                                                                                 tataAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACa                                           <  1:460863/140‑1 (MQ=255)                                                                                                  ataAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTGAGTATTTTACCTCCTACAATAGCTTTATAAAACaa                                          >  1:174003/1‑140 (MQ=255)                                                                                                    aaaTGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAg                                        <  1:763091/140‑1 (MQ=255)                                                                                                      aTGGCTATAGCCCATTCCATACTTTATAAAAGTATGGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGGTAATTATTTTACCTCCTACAACAGCTTTATAAAACAAAGaa                                      <  1:562206/140‑1 (MQ=255)                                                                                                       tGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGaaa                                     <  1:802328/140‑1 (MQ=255)                                                                                                              aGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTttaa                              >  2:136501/1‑140 (MQ=255)                                                                                                               gCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGGCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTttaat                             >  1:691179/1‑140 (MQ=255)                                                                                                                  cATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACACAGAAAATTttaattaa                          <  1:497707/140‑1 (MQ=255)                                                                                                                  cATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTttaattaa                          >  1:822008/1‑140 (MQ=255)                                                                                                                      tcATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAata                      <  2:163764/139‑1 (MQ=255)                                                                                                                        aTACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAatata                    >  1:565524/1‑140 (MQ=255)                                                                                                                        aTACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAatata                    >  1:565573/1‑140 (MQ=255)                                                                                                                         tACTTTGTAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAatatat                   <  1:849577/140‑1 (MQ=255)                                                                                                                           cTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTCTAATTCAAAGAATAGTTTAATTAAAATATATAg                 <  1:486142/140‑1 (MQ=255)                                                                                                                              tataAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAgcgc              <  1:622117/140‑1 (MQ=255)                                                                                                                              taaaaaaGTAACGGAAATGGGCTATTTGACGTGCGATGAATTTTAGTACACTTTTCATATTCTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAgcgc              <  2:65177/137‑1 (MQ=255)                                                                                                                                  aaaGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACTa          >  2:518639/1‑140 (MQ=255)                                                                                                                                     gTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGACAATTTTACTTAAAATATCTAGCGCACTAtat       >  1:851782/1‑138 (MQ=255)                                                                                                                                     gTATCGGAAAGGGGCTAGTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACTATGt       <  1:127592/140‑1 (MQ=255)                                                                                                                                      tagcGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTTATTAAAATAAATAGCGCACTATGtt      <  1:503601/137‑1 (MQ=255)                                                                                                                                      tATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACTATGtt      <  1:866966/140‑1 (MQ=255)                                                                                                                                      tATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACTATGtt      <  1:79789/140‑1 (MQ=255)                                                                                                                                      tATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACTATGtt      <  1:491378/140‑1 (MQ=255)                                                                                                                                        tCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACTATGTtat    >  1:264702/1‑140 (MQ=255)                                                                                                                                         gggAAATGGGCTATTTGTTGTGAGATGAATGTTAGTACACTTTTCATATTTTGGAAAGACATGTTAATTATTTTACATCCTACAATAGCTTTATATAAAAACGAGAATTTTAATTAAAATATATGGAGAACTATGTtgta   >  2:513759/2‑140 (MQ=255)                                                                                                                                         cGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACTATGTtata   >  2:820432/1‑140 (MQ=255)                                                                                                                                         cGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACTATGTtata   >  1:1005526/1‑140 (MQ=255)                                                                                                                                         cGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACAATGTtata   >  2:243115/1‑140 (MQ=255)                                                                                                                                          ggAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACTATGTTATaa  <  1:390623/140‑1 (MQ=255)                                                                                                                                          ggAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTACTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACTATGTTATaa  >  1:426197/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                          CACCTTCAGAAATTGGTTCAACATTAATTGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAACTGTTAATAAATAAAGTTAAAAGATGATATAAATGGCTATAGCCCATTCCATACTTTATAAAAGTATCGGAAATGGGCTATTTGTCGTGCGATGAATTTTAGTACACTTTTCATATTTTGGAAAGACAAGTTAATTATTTTACCTCCTACAATAGCTTTATAAAACAAAGAAAATTTTAATTAAAATATATAGCGCACTATGTTATAA  >  USA300TCH1516_ALE/1262793‑1263069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.