Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	USA300TCH1516_ALE	2,501,188	Δ3,014 bp		melR_2–ydaG	melR_2, ydaG

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	USA300TCH1516_ALE	2501188	2504201	3014	94 [0]	[0] 96	melR_2–ydaG	melR_2,ydaG

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 2501187	0 (0.000)	91 (1.140)	62/250	0.1	100%	intergenic (+1198/‑290)	USA300HOU_RS12875/melR_2	hypothetical protein/Melibiose operon regulatory protein
	?	USA300TCH1516_ALE	2504202 =	0 (0.000)					intergenic (+127/‑138)	ydaG/USA300HOU_RS12890	General stress protein 26/hypothetical protein

GATAAAATTTAAGTCATGGGTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTGAAATACATAGGTTTGAAATCTATATTAATTATGCACCAAATAATTTAATTAGACTCTATCGAAAATTTCCAAATTTTGCTTACTATCTTTCTAATTTTACTTTTATATTGTTCGCAATGCATAAAAACAAGCGTTT  >  USA300TCH1516_ALE/2501050‑2501324                                                                                                                                          |                                                                                                                                          gATAAAATTTAAGTCATGGGTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTag                                                                                                                                         <  1:528381‑M1/140‑3 (MQ=255)     aaaTTTAAGTCATGGGTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacac                                                                                                                                     >  2:625270‑M1/1‑134 (MQ=255)      aaTTTAAGTCATGGGTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCACTAAAAGATTAGCAAGTTAGTTCAAAATGGCTACGTATTGTTTTTTagacaca                                                                                                                                    >  2:75193‑M1/1‑133 (MQ=255)       aTTTAAGTTATGAGTAAATTTAATATTTTTTATTACAAACTTCCACTTAGGTCAATTCGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacac                                                                                                                                   <  1:1751‑M1/140‑9 (MQ=255)       aTTTAAGTCATGGGTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTATCTAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacac                                                                                                                                   <  2:659889‑M1/140‑9 (MQ=255)       aTTTAAGTCATGGGTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacaccc                                                                                                                                   >  1:147669‑M1/1‑132 (MQ=255)       aTTTAAGTCATGGGTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacac                                                                                                                                   <  2:759905‑M1/140‑9 (MQ=255)             gTCATGGGTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttatt                                                                                                                             >  2:818487‑M1/1‑126 (MQ=255)             gTCATGGGTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttatt                                                                                                                             <  1:246525‑M1/140‑15 (MQ=255)                  gggTACATTTAATGTTTTTTGTTTCTAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTTAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTCTTGTTTTTTcgacacactttattcacat                                                                                                                        >  2:888561‑M1/1‑121 (MQ=255)                  gggTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTTTTTTTTTTTTTTATATTAATATAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacat                                                                                                                        >  2:181030‑M1/1‑121 (MQ=255)                  gggTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGAAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacat                                                                                                                        >  2:384990‑M1/1‑121 (MQ=255)                   ggTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatt                                                                                                                       <  2:242512‑M1/140‑21 (MQ=255)                      aaattTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaa                                                                                                                    <  2:821683‑M1/138‑24 (MQ=255)                      aCATTTAATGTTTTTTTATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaa                                                                                                                    <  2:915239‑M1/140‑24 (MQ=255)                      aCATTTAATGTTTTTTGCTTCGTTCTCCCATTTTGGTCAAGTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAATTCAAAATGGCTATGTATTGTTTTTTagacacacattattcacatttaa                                                                                                                    >  1:486856‑M1/1‑117 (MQ=255)                      aCATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaa                                                                                                                    <  1:555364‑M1/140‑24 (MQ=255)                         tttAATGTTTTTTGATTCAATCTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgt                                                                                                                 <  1:356540‑M1/140‑27 (MQ=255)                         tttAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgt                                                                                                                 <  1:333285‑M1/140‑27 (MQ=255)                             aTGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgatt                                                                                                             >  1:342159‑M1/1‑110 (MQ=255)                              tGTTTTTTGATTCAAACTTCCATTTTGGCCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgatta                                                                                                            <  1:1000616‑M1/140‑32 (MQ=255)                                  ttttGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggt                                                                                                        <  2:664366‑M1/140‑36 (MQ=255)                                     tGATTCAATCTTTGATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATGAATAAATGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtg                                                                                                     <  2:280463‑M1/140‑39 (MQ=255)                                      gATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgt                                                                                                    <  2:410258‑M1/140‑40 (MQ=255)                                             aCTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtcttttta                                                                                             <  1:892764‑M1/140‑47 (MQ=255)                                              cTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaa                                                                                            <  1:869963‑M1/140‑48 (MQ=255)                                               ttCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaa                                                                                           <  2:979827‑M1/140‑49 (MQ=255)                                                tCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaat                                                                                          <  1:298246‑M1/140‑50 (MQ=255)                                                tCCATTTTGGTCAATTCGCTTATTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGAATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaat                                                                                          >  1:516994‑M1/1‑91 (MQ=255)                                                 ccATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAACTGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaatt                                                                                         <  1:136890‑M1/140‑51 (MQ=255)                                                 ccATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtttgtctttttaagtt                                                                                         >  1:422206‑M1/1‑90 (MQ=255)                                                 ccATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaatt                                                                                         <  1:714801‑M1/140‑51 (MQ=255)                                                 ccATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaatt                                                                                         <  1:145130‑M1/140‑51 (MQ=255)                                                 ccATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaatt                                                                                         >  1:840037‑M1/1‑90 (MQ=255)                                                 ccATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAACATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaatt                                                                                         >  1:664641‑M1/1‑90 (MQ=255)                                                 ccATTTTGGTCAATTTGCTTTTTTGATAACATAGTTGACTATCAATAAAAGATTAGCAAGTTAGTTCAAGATGGCTATGTATTGTTTTTTagacacactttattcacctttaacgtgattagggtgtgtctttttgaatt                                                                                         >  1:645823‑M1/1‑90 (MQ=255)                                                  ctttttggTCAATTTGCTTTTTTTATAACATAGCTGAATATCAACAAAGGATGAGCTAGTTAGTTCAAAATGGCTATGTATTTTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaatta                                                                                        <  1:380515‑M1/138‑52 (MQ=255)                                                  cactttggtCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaatta                                                                                        <  1:359665‑M1/137‑52 (MQ=255)                                                  cATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaatta                                                                                        <  1:664967‑M1/140‑52 (MQ=255)                                                   aTTTTGGTTAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaa                                                                                       <  2:314279‑M1/140‑53 (MQ=255)                                                    ttttGGTCAATTTGCTTTTTTTATAACATAGTTTAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgggtctttttaaattaat                                                                                      <  2:865176‑M1/140‑54 (MQ=255)                                                          tCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatg                                                                                <  2:987752‑M1/140‑60 (MQ=255)                                                           cAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgt                                                                               <  1:927255‑M1/140‑61 (MQ=255)                                                                tGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatat                                                                          <  2:275775‑M1/140‑66 (MQ=255)                                                                tGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatat                                                                          <  1:425090‑M1/140‑66 (MQ=255)                                                                  cTTTTTTTATAACTTAGTTGACTATCAATAAAAGATGAGGAAGTTAGTTAAAAATTGCTATGTATTGTTTTTTaggcacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataa                                                                        <  2:128769‑M1/140‑68 (MQ=255)                                                                     tttttATAACATAGTTGAATATAAGTAAAAGCTTAGCAAGTTCGTTCAACATGGCTATGTTTTGTTTTTTagacacactttattcacatttaacgtgattatggtgtgtcttttttaattaattttctttattataaata                                                                     >  2:298084‑M1/1‑70 (MQ=255)                                                                      ttttATAACTTAGTTGAATATTAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatac                                                                    <  1:200988‑M1/140‑72 (MQ=255)                                                                      ttttATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatac                                                                    <  1:120312‑M1/140‑72 (MQ=255)                                                                        ttATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatttaaatacgg                                                                  >  1:73821‑M1/1‑67 (MQ=255)                                                                          ataACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtatttagggtgtgtctttttaaattaattttatgtaatataaatactgca                                                                >  1:338040‑M1/1‑65 (MQ=255)                                                                           taACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcat                                                               >  2:311842‑M1/1‑64 (MQ=255)                                                                           taACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgggtctttttaaattaattttatgtaatataaatactcaat                                                               >  2:36374‑M1/1‑64 (MQ=255)                                                                              cATAGTTGAATATCACTAAAAGATTAGCAAGTTAGTTTAAAATGGCTATGTATTGTTTTTTagacacactttattcacattaaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttg                                                            <  1:282573‑M1/140‑80 (MQ=255)                                                                                 aGTTGAATATCAATAAAAGATTAGCTAGTTAGTTCATATTGGCTATGTATTGTTTTTTagacacactttattcacatttaacgcgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaa                                                         <  1:837358‑M1/140‑83 (MQ=255)                                                                                 aGTTGAATATCAATAAAAGATTAGCAAGTTATTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaa                                                         <  1:70531‑M1/140‑83 (MQ=255)                                                                                 aGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaa                                                         <  1:603307‑M1/140‑83 (MQ=255)                                                                                     gAATATTAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactg                                                     <  2:951034‑M1/140‑87 (MQ=255)                                                                                     gAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttattttatttttatgtaatataaatactgcgtttgcaaactg                                                     >  2:393356‑M1/1‑54 (MQ=255)                                                                                     gAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactggattggctaactg                                                     >  1:677144‑M1/1‑54 (MQ=255)                                                                                      aaTATCAATAAAAGATTAGCAAGTTAGTTCAAAATGACTATGTATTGTTTTTTagacacactttattcacatttaacgtggttagggtgtgtctttttaaattaattttatgtaatataaatactgcattttaaaagtgt                                                    >  2:531655‑M1/1‑53 (MQ=255)                                                                                       atatCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgtt                                                   <  1:357354‑M1/140‑89 (MQ=255)                                                                                       atatCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgaaatataaatactgcatttgcaaactgtt                                                   >  2:546473‑M1/1‑52 (MQ=255)                                                                                        tatTAAAAAAAGATTAGCAAGTTAGTTAAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttg                                                  <  2:543162‑M1/140‑90 (MQ=255)                                                                                         atCAATAAAAGATTAGCAAGTTAGTTCAACATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaacggttgc                                                 <  2:792472‑M1/140‑91 (MQ=255)                                                                                         atCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgc                                                 <  2:165681‑M1/140‑91 (MQ=255)                                                                                           cAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggagagactattaaaattaattttatgtaatataattacctcatttgcaaactgttgcac                                               >  2:654720‑M1/1‑48 (MQ=255)                                                                                              tAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttt                                            >  2:544345‑M1/1‑45 (MQ=255)                                                                                                aaaGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcactttta                                          <  1:55248‑M1/140‑98 (MQ=255)                                                                                                   gagaagcaaGATAGGTAAAAATGGCTATGTATTGATCTAAagaaacactttattcacatttaacgtgattagagtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggt                                       <  1:72174‑M1/136‑101 (MQ=255)                                                                                                    aTTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggta                                      <  1:367859‑M1/140‑102 (MQ=255)                                                                                                    aTTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggta                                      <  1:597659‑M1/140‑102 (MQ=255)                                                                                                    aTTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggta                                      <  1:870171‑M1/140‑102 (MQ=255)                                                                                                       aGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataa                                   <  1:253424‑M1/140‑105 (MQ=255)                                                                                                       aGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacacttgattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataa                                   <  2:759779‑M1/140‑105 (MQ=255)                                                                                                       aGCAAGTTAGTTCAAAATGGCTATGCATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtatctttttaaattaattttatgtaatataaatactcgtaatgcaaactgttgcacttatacttataa                                   <  1:738467‑M1/140‑105 (MQ=255)                                                                                                        gctatttagttcaaaaTGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataac                                  <  2:268470‑M1/135‑106 (MQ=255)                                                                                                        gCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaaaactgcatttgcaaactgttgcacttttaggtataac                                  >  1:414112‑M1/1‑35 (MQ=255)                                                                                                        gCAAGTTAGTTCAAAATGCCTATGTATTGTTTTTTagacactctttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataac                                  <  2:37409‑M1/140‑106 (MQ=255)                                                                                                          aaGTTAGTTCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataacag                                <  1:818487‑M1/140‑108 (MQ=255)                                                                                                           agttagttCAAAATGGCTATGGATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatgtgcaaactgttgcacttttaggtataacaga                               <  1:198097‑M1/140‑109 (MQ=255)                                                                                                             ttagttCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataacagaat                             >  1:484899‑M1/1‑30 (MQ=255)                                                                                                              tagttCACAATGGCTATTTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataacagaatt                            <  1:546593‑M1/140‑112 (MQ=255)                                                                                                              tagttCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataacagaatt                            <  1:855607‑M1/140‑112 (MQ=255)                                                                                                               agttCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaatttattttatgtaatataaatactgcatttgcaacctgttgcacttttacgtataacagaatta                           <  1:536564‑M1/140‑113 (MQ=255)                                                                                                                 ttCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttattttatataaatactgcatttgcaaactgttgcacttttagatatatccgaattaat                         >  2:403028‑M1/1‑26 (MQ=255)                                                                                                                 ttCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataacagaattaac                         >  2:116862‑M1/1‑26 (MQ=255)                                                                                                                 ttCAAAATGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataacagaattaac                         >  2:977226‑M1/1‑26 (MQ=255)                                                                                                                     aaaTGGCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataacagaattaactaca                     >  2:446166‑M1/1‑22 (MQ=255)                                                                                                                          gCTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataacagaattaactacatttaa                <  1:885277‑M1/140‑124 (MQ=255)                                                                                                                           cTATGTATTGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataacagaattaactacatttaag               <  2:901716‑M1/140‑125 (MQ=255)                                                                                                                                  ttGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataacagaattaactacatttaaggagatgg        >  2:914026‑M1/1‑9 (MQ=255)                                                                                                                                  ttGTTTTTTagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataacagaattaactacatttaagaagattg        >  2:279307‑M1/1‑9 (MQ=255)                                                                                                                                        tttagacacactttattcacatttaacgtgattagggtgtgtctttttaaattaattttatgtaatataaatactgcatttgcaaactgttgcacttttaggtataacagaattaactacatttaaggagattgatgaac  >  2:382304‑M1/1‑3 (MQ=255)                                                                                                                                          |                                                                                                                                          GATAAAATTTAAGTCATGGGTACATTTAATGTTTTTTGATTCAAACTTCCATTTTGGTCAATTTGCTTTTTTTATAACATAGTTGAATATCAATAAAAGATTAGCAAGTTAGTTCAAAATGGCTATGTATTGTTTTTTGAAATACATAGGTTTGAAATCTATATTAATTATGCACCAAATAATTTAATTAGACTCTATCGAAAATTTCCAAATTTTGCTTACTATCTTTCTAATTTTACTTTTATATTGTTCGCAATGCATAAAAACAAGCGTTT  >  USA300TCH1516_ALE/2501050‑2501324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑