New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	3782590 =	33 (1.100)	5 (0.180)	5/270	3.3	14.1%	intergenic (‑28/+52)	cysE/gpsA	serine acetyltransferase/glycerol‑3‑phosphate dehydrogenase (NAD+)
	?	NC_000913_3_bme_pgi	3782613 =	30 (1.060)					intergenic (‑51/+29)	cysE/gpsA	serine acetyltransferase/glycerol‑3‑phosphate dehydrogenase (NAD+)
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTTCGTGCTTGAGTAGCGTCGCGTGGTAAAAACTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTC  >  NC_000913_3_bme_pgi/3782445‑3782737                                                                                                                                                  |                                                                                                                                                    tttCGTGCTTGAGTAGCGTCGCGTGGTAAAAACTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCgg                                                                                                                                                  <  1:447777/149‑1 (MQ=255)        ctggAGTAGCGTCGCGTGGTAAAAACTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTc                                                                                                                                           <  2:13947/146‑1 (MQ=255)        cTTGAGTAGCGTCGCGTGGTAAAAACTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTc                                                                                                                                           >  1:242087/1‑149 (MQ=255)             gTAGCGTCGCGTGGTAAAAACTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCGCACGATGAGATAATGACCGGCCCATTCTgcgc                                                                                                                                      >  1:83119/1‑149 (MQ=255)                    cgcgTGGTAAAAACTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCgg                                                                                                                               <  2:400199/149‑1 (MQ=255)                             aaaaCTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATAGTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCg                                                                                                                      <  1:204573/149‑1 (MQ=255)                                 cTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTaa                                                                                                                  <  1:463237/149‑1 (MQ=255)                                     ccAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACaaa                                                                                                              >  2:35256/1‑149 (MQ=255)                                     ccAGCATGGGCTCAGAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACaaa                                                                                                              <  1:206430/149‑1 (MQ=255)                                               cTCACAGTCCGCCAGCGTTCTGGCTTAGGCTTTAATATTGTACCAGACAATTTCCAGTTCTGCATACGCCATTGCTTACTCCACACGATGAGATAATGACCGGCCCTTTCCGAGCGGGCCGGGTCATAGCGGAAACAAAGATTCCCTgg                                                                                                    >  2:194247/1‑149 (MQ=255)                                                tCACATTCCCTCAGGGTTGTGGCTTCGGCGTTAATCTTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTggg                                                                                                   <  2:203217/149‑1 (MQ=255)                                                  acaGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTGCTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGt                                                                                                 <  2:411639/149‑1 (MQ=255)                                                         gCCAGCGTTCTGTCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGgc                                                                                          <  1:285679/149‑1 (MQ=255)                                                             tcGTTCTGGCGTCGGCTTTAATATGGTTCCAGTCAATTTCCAGTTCTTCACACGACATTGCTTCCTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGgctgct                                                                                      <  2:294708/148‑1 (MQ=255)                                                                                aaTATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCa                                                                   <  1:491265/149‑1 (MQ=255)                                                                                   atTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTGACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGa                                                                >  1:205353/1‑149 (MQ=255)                                                                                       ttCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTa                                                            >  1:11898/1‑149 (MQ=255)                                                                                          cAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAg                                                                                              <  1:256993/112‑1 (MQ=255)                                                                                          cAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAg                                                                                              >  2:256993/1‑112 (MQ=255)                                                                                           aGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCgg                                                                                                                                    >  2:178964/1‑73 (MQ=255)                                                                                           aGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCgg                                                                                                                                    <  1:178964/73‑1 (MQ=255)                                                                                               aaTTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGCCATCGTAAAGTc                                                    >  2:320435/1‑149 (MQ=255)                                                                                                  ttCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTCGTAAAGTCAAt                                                 >  1:505776/1‑149 (MQ=255)                                                                                                            tcACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTcgc                                       <  1:375833/149‑1 (MQ=255)                                                                                                                   aCATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACACAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTTGACGACCTAGTACAGTCCATGCTGCCACGCGCGCCtt                                >  2:469138/1‑149 (MQ=255)                                                                                                                     aTTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAATCAATGCTGCCTCGCGCGCGtttt                              >  2:70539/1‑149 (MQ=255)                                                                                                                            aCTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGc                                                                        >  2:283939/1‑100 (MQ=255)                                                                                                                            aCTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGc                                                                        <  1:283939/100‑1 (MQ=255)                                                                                                                                 acacGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAAtata                  >  1:117363/1‑149 (MQ=255)                                                                                                                                    cGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCTTTGCTTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATataata               <  1:519547/149‑1 (MQ=255)                                                                                                                                     gATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTAAATGCTGCCTCGCGCGCGTTTTTTCCGCAATataatca              >  1:208956/1‑147 (MQ=255)                                                                                                                                         agaTAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGa          >  2:20516/1‑149 (MQ=255)                                                                                                                                                 aCCGGCGCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCTCTCGTCCTTGCGTGCACGATCTAGTAAATTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTc  <  2:456320/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TTTCGTGCTTGAGTAGCGTCGCGTGGTAAAAACTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTC  >  NC_000913_3_bme_pgi/3782445‑3782737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.