New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi 3782590 =33 (1.100)5 (0.180) 5/270 3.3 14.1% intergenic (‑28/+52) cysE/gpsA serine acetyltransferase/glycerol‑3‑phosphate dehydrogenase (NAD+)
?NC_000913_3_bme_pgi 3782613 = 30 (1.060)intergenic (‑51/+29) cysE/gpsA serine acetyltransferase/glycerol‑3‑phosphate dehydrogenase (NAD+)
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAAACTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAAC  >  NC_000913_3_bme_pgi/3782473‑3782758
                                                                                                                                            |                                                                                                                                                 
aaaaCTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATAGTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCg                                                                                                                                           <  1:204573/149‑1 (MQ=255)
    cTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTaa                                                                                                                                       <  1:463237/149‑1 (MQ=255)
        ccAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACaaa                                                                                                                                   >  2:35256/1‑149 (MQ=255)
        ccAGCATGGGCTCAGAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACaaa                                                                                                                                   <  1:206430/149‑1 (MQ=255)
                  cTCACAGTCCGCCAGCGTTCTGGCTTAGGCTTTAATATTGTACCAGACAATTTCCAGTTCTGCATACGCCATTGCTTACTCCACACGATGAGATAATGACCGGCCCTTTCCGAGCGGGCCGGGTCATAGCGGAAACAAAGATTCCCTgg                                                                                                                         >  2:194247/1‑149 (MQ=255)
                   tCACATTCCCTCAGGGTTGTGGCTTCGGCGTTAATCTTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTggg                                                                                                                        <  2:203217/149‑1 (MQ=255)
                     acaGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTGCTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGt                                                                                                                      <  2:411639/149‑1 (MQ=255)
                            gCCAGCGTTCTGTCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGgc                                                                                                               <  1:285679/149‑1 (MQ=255)
                                tcGTTCTGGCGTCGGCTTTAATATGGTTCCAGTCAATTTCCAGTTCTTCACACGACATTGCTTCCTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGgctgct                                                                                                           <  2:294708/148‑1 (MQ=255)
                                                   aaTATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCa                                                                                        <  1:491265/149‑1 (MQ=255)
                                                      atTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTGACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGa                                                                                     >  1:205353/1‑149 (MQ=255)
                                                          ttCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTa                                                                                 >  1:11898/1‑149 (MQ=255)
                                                             cAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAg                                                                                                                   >  2:256993/1‑112 (MQ=255)
                                                             cAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAg                                                                                                                   <  1:256993/112‑1 (MQ=255)
                                                                  aaTTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGCCATCGTAAAGTc                                                                         >  2:320435/1‑149 (MQ=255)
                                                                     ttCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTCGTAAAGTCAAt                                                                      >  1:505776/1‑149 (MQ=255)
                                                                               tcACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTcgc                                                            <  1:375833/149‑1 (MQ=255)
                                                                                      aCATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACACAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTTGACGACCTAGTACAGTCCATGCTGCCACGCGCGCCtt                                                     >  2:469138/1‑149 (MQ=255)
                                                                                        aTTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAATCAATGCTGCCTCGCGCGCGtttt                                                   >  2:70539/1‑149 (MQ=255)
                                                                                               aCTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGc                                                                                             >  2:283939/1‑100 (MQ=255)
                                                                                               aCTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGc                                                                                             <  1:283939/100‑1 (MQ=255)
                                                                                                    acacGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAAtata                                       >  1:117363/1‑149 (MQ=255)
                                                                                                       cGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCTTTGCTTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATataata                                    <  1:519547/149‑1 (MQ=255)
                                                                                                        gATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTAAATGCTGCCTCGCGCGCGTTTTTTCCGCAATataatca                                   >  1:208956/1‑147 (MQ=255)
                                                                                                            agaTAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGa                               >  2:20516/1‑149 (MQ=255)
                                                                                                                    aCCGGCGCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCTCTCGTCCTTGCGTGCACGATCTAGTAAATTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTc                       <  2:456320/149‑1 (MQ=255)
                                                                                                                        gCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCg                   <  1:40352/149‑1 (MQ=255)
                                                                                                                                tGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTCTTgg           >  1:228311/1‑149 (MQ=255)
                                                                                                                                 gcgcGGGGCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGc          <  1:35256/149‑1 (MQ=255)
                                                                                                                                  cgcgGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCa         <  2:11898/149‑1 (MQ=255)
                                                                                                                                     gggCGGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCAttt      <  2:242087/149‑1 (MQ=255)
                                                                                                                                     gggCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTTATAAATTTCCTCGGATATAGGCAttt      >  1:148616/1‑149 (MQ=255)
                                                                                                                                         cGGGTCATAGCGGTAACAAAGGTTCCCTGGGGATAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCCAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCCTTTCacc  >  1:504380/1‑147 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
AAAACTGGCCAGCATTGGCTCACAGTCCGCCAGCGTTCTGGCTTCGGCTTTAATATTGTTCCAGACAATTTCCAGTTCTTCACACGACATTGCTTACTCCACACGATGAGATAATGACCGGCCCGTTCTGCGCGGGCCGGGTCATAGCGGTAACAAAGGTTCCCTGGGGTTAGTGGCTGCTGCGCTCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAAC  >  NC_000913_3_bme_pgi/3782473‑3782758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.