New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 3226187	62 (0.970)	8 (0.130)	8/262	7.5	12.3%	intergenic (+16/‑47)	ebgC/ygjI	evolved beta‑D‑galactosidase, beta subunit; cupin superfamily/putative transporter
	?	NC_000913_3_KO_pgi	= 3226192	56 (0.930)					intergenic (+21/‑42)	ebgC/ygjI	evolved beta‑D‑galactosidase, beta subunit; cupin superfamily/putative transporter
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAG  >  NC_000913_3_KO_pgi/3226038‑3226335                                                                                                                                                      |                                                                                                                                                     aaaCCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCaaa                                                                                                                                                      >  1:18663/1‑150 (MQ=255)   aCCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAg                                                                                                                                                    <  1:404933/150‑1 (MQ=255)         gAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGttt                                                                                                                                              <  2:195196/150‑1 (MQ=255)          aGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTg                                                                                                                                             <  3:134828/150‑1 (MQ=255)            gTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgcc                                                                                                                                           >  3:52581/1‑150 (MQ=255)               cACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgcc                                                                                                                                        >  2:57887/1‑150 (MQ=255)               cACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgcc                                                                                                                                        >  3:339058/1‑150 (MQ=255)                            tCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTCTTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTAc                                                                                                                           >  4:216749/1‑150 (MQ=255)                              gTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTc                                                                                                                         >  4:163955/1‑150 (MQ=255)                                tATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCAt                                                                                                                       <  2:382529/150‑1 (MQ=255)                                  tttGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTCCCCTACTCAttt                                                                                                                     >  2:228727/1‑150 (MQ=255)                                     gCGATATCCATGAAGCGTAGCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg                                                                                                                  <  1:550545/150‑1 (MQ=255)                                       gATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGga                                                                                                                <  2:672012/150‑1 (MQ=255)                                       gATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGga                                                                                                                >  2:9774/1‑150 (MQ=255)                                       gATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGga                                                                                                                <  1:649570/150‑1 (MQ=255)                                          atCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAt                                                                                                             <  2:609925/150‑1 (MQ=255)                                          atCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAt                                                                                                             <  2:576990/150‑1 (MQ=255)                                             cATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAtgtg                                                                                                          >  2:254344/1‑150 (MQ=255)                                                gAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTa                                                                                                       <  1:29485/150‑1 (MQ=255)                                                   gCGTATCGGTTTATCTGCAATAACGCGATCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGt                                                                                                    >  1:490955/1‑150 (MQ=255)                                                     gTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCt                                                                                                  <  1:57887/150‑1 (MQ=255)                                                               aTCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAAc                                                                                        <  1:121683/150‑1 (MQ=255)                                                                   gCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTaa                                                                                    >  4:99385/1‑150 (MQ=255)                                                                     aaTAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATa                                                                                  >  4:131947/1‑150 (MQ=255)                                                                          cgcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTAt                                                                                                      <  2:185084/125‑1 (MQ=255)                                                                          cgcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTAt                                                                                                      >  1:185084/1‑125 (MQ=255)                                                                           gcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCAtttt                                                                                                                    >  3:81085/1‑110 (MQ=255)                                                                           gcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCAtttt                                                                                                                    <  4:81085/110‑1 (MQ=255)                                                                           gcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCg                                                                            <  1:113313/150‑1 (MQ=255)                                                                                 aaaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAAt                                                                      <  4:215364/150‑1 (MQ=255)                                                                                    aaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCg                                                                   <  1:286734/150‑1 (MQ=255)                                                                                       gTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCt                                                                <  1:9774/150‑1 (MQ=255)                                                                                            tctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGctc                                                           >  2:110911/1‑150 (MQ=255)                                                                                             ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGctct                                                          >  3:70339/1‑150 (MQ=255)                                                                                             ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGctct                                                          <  1:76316/150‑1 (MQ=255)                                                                                             ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGctct                                                          >  1:1487/1‑150 (MQ=255)                                                                                                aaaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGc                                                       <  2:222241/150‑1 (MQ=255)                                                                                                aaaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCCACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAACCGGCAAATTCGGCTTGCCCTCGc                                                       >  4:127905/1‑150 (MQ=255)                                                                                                 aaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCt                                                      <  1:110401/150‑1 (MQ=255)                                                                                                  aGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTg                                                     >  1:560556/1‑150 (MQ=255)                                                                                                   gTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGa                                                    <  2:490955/150‑1 (MQ=255)                                                                                                         aTCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCaaa                                                                                         <  3:112779/107‑1 (MQ=255)                                                                                                         aTCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCaaa                                                                                         >  4:112779/1‑107 (MQ=255)                                                                                                          tCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTgc                                             >  4:50813/1‑150 (MQ=255)                                                                                                           cGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTgcc                                            <  2:577351/150‑1 (MQ=255)                                                                                                            gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTTGCTGACTTTTgccg                                           >  3:73833/1‑150 (MQ=255)                                                                                                            gAAGATGGGTATTTCCATAACAATTACCAGATTCACCTGCGAGGGTAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGGGTTATGTCTGATACCAAACGGAAGACAATGTACAGATTCGGCTGGCTCTCGCTGACttttacag                                           >  4:325749/1‑146 (MQ=255)                                                                                                                   gTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTa                                    <  2:18663/150‑1 (MQ=255)                                                                                                                          ccATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCttt                             >  2:52234/1‑150 (MQ=255)                                                                                                                          ccATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCttt                             >  2:306804/1‑150 (MQ=255)                                                                                                                           cATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACa                                  >  3:331013/1‑144 (MQ=255)                                                                                                                           cATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACa                                  <  4:331013/144‑1 (MQ=255)                                                                                                                            ataacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTACTACAATCGGCAAATTCGGCTTGCTCTCGCTGTCTTTTGCCGCCGTTTACAGCTTTaa                           <  4:232598/150‑1 (MQ=255)                                                                                                                            ataacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTaa                           <  4:70339/150‑1 (MQ=255)                                                                                                                             taacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTaac                          >  1:342401/1‑150 (MQ=255)                                                                                                                             taacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGATTACAGCTTTaac                          >  2:599067/1‑150 (MQ=255)                                                                                                                                caaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTaacaac                       <  3:81299/150‑1 (MQ=255)                                                                                                                                 aaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGtt                                                                                                        >  2:680484/1‑68 (MQ=255)                                                                                                                                 aaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGtt                                                                                                        <  1:680484/68‑1 (MQ=255)                                                                                                                                      gcaacTACGGCGGCAACAGGAGTTGGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAAGACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATc                 <  3:281273/149‑1 (MQ=255)                                                                                                                                               gcggcAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCaataataat        <  4:52581/150‑1 (MQ=255)                                                                                                                                                    aaaaGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGa   >  3:297665/1‑150 (MQ=255)                                                                                                                                                     aaaGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAg  <  2:560556/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     AAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAG  >  NC_000913_3_KO_pgi/3226038‑3226335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.