New junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913_3_KO_pgi | = 3226187 | 62 (0.970) | 8 (0.130) | 8/262 | 7.5 | 12.3% | intergenic (+16/‑47) | ebgC/ygjI | evolved beta‑D‑galactosidase, beta subunit; cupin superfamily/putative transporter |
? | NC_000913_3_KO_pgi | = 3226192 | 56 (0.930) | intergenic (+21/‑42) | ebgC/ygjI | evolved beta‑D‑galactosidase, beta subunit; cupin superfamily/putative transporter | |||||
Rejected: Coverage evenness skew score above cutoff. | |||||||||||
Rejected: Frequency below/above cutoff threshold. |
GAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAG > NC_000913_3_KO_pgi/3226046‑3226335 | gAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGttt < 2:195196/150‑1 (MQ=255) aGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTg < 3:134828/150‑1 (MQ=255) gTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgcc > 3:52581/1‑150 (MQ=255) cACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgcc > 3:339058/1‑150 (MQ=255) cACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgcc > 2:57887/1‑150 (MQ=255) tCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTCTTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTAc > 4:216749/1‑150 (MQ=255) gTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTc > 4:163955/1‑150 (MQ=255) tATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCAt < 2:382529/150‑1 (MQ=255) tttGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTCCCCTACTCAttt > 2:228727/1‑150 (MQ=255) gCGATATCCATGAAGCGTAGCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg < 1:550545/150‑1 (MQ=255) gATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGga > 2:9774/1‑150 (MQ=255) gATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGga < 1:649570/150‑1 (MQ=255) gATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGga < 2:672012/150‑1 (MQ=255) atCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAt < 2:609925/150‑1 (MQ=255) atCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAt < 2:576990/150‑1 (MQ=255) cATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAtgtg > 2:254344/1‑150 (MQ=255) gAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTa < 1:29485/150‑1 (MQ=255) gCGTATCGGTTTATCTGCAATAACGCGATCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGt > 1:490955/1‑150 (MQ=255) gTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCt < 1:57887/150‑1 (MQ=255) aTCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAAc < 1:121683/150‑1 (MQ=255) gCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTaa > 4:99385/1‑150 (MQ=255) aaTAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATa > 4:131947/1‑150 (MQ=255) cgcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTAt < 2:185084/125‑1 (MQ=255) cgcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTAt > 1:185084/1‑125 (MQ=255) gcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCAtttt < 4:81085/110‑1 (MQ=255) gcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCAtttt > 3:81085/1‑110 (MQ=255) gcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCg < 1:113313/150‑1 (MQ=255) aaaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAAt < 4:215364/150‑1 (MQ=255) aaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCg < 1:286734/150‑1 (MQ=255) gTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCt < 1:9774/150‑1 (MQ=255) tctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGctc > 2:110911/1‑150 (MQ=255) ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGctct > 1:1487/1‑150 (MQ=255) ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGctct < 1:76316/150‑1 (MQ=255) ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGctct > 3:70339/1‑150 (MQ=255) aaaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGc < 2:222241/150‑1 (MQ=255) aaaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCCACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAACCGGCAAATTCGGCTTGCCCTCGc > 4:127905/1‑150 (MQ=255) aaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCt < 1:110401/150‑1 (MQ=255) aGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTg > 1:560556/1‑150 (MQ=255) gTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGa < 2:490955/150‑1 (MQ=255) aTCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCaaa < 3:112779/107‑1 (MQ=255) aTCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCaaa > 4:112779/1‑107 (MQ=255) tCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTgc > 4:50813/1‑150 (MQ=255) cGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTgcc < 2:577351/150‑1 (MQ=255) gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTTGCTGACTTTTgccg > 3:73833/1‑150 (MQ=255) gAAGATGGGTATTTCCATAACAATTACCAGATTCACCTGCGAGGGTAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGGGTTATGTCTGATACCAAACGGAAGACAATGTACAGATTCGGCTGGCTCTCGCTGACttttacag > 4:325749/1‑146 (MQ=255) gTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTa < 2:18663/150‑1 (MQ=255) ccATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCttt > 2:52234/1‑150 (MQ=255) ccATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCttt > 2:306804/1‑150 (MQ=255) cATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACa > 3:331013/1‑144 (MQ=255) cATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACa < 4:331013/144‑1 (MQ=255) ataacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTACTACAATCGGCAAATTCGGCTTGCTCTCGCTGTCTTTTGCCGCCGTTTACAGCTTTaa < 4:232598/150‑1 (MQ=255) ataacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTaa < 4:70339/150‑1 (MQ=255) taacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTaac > 1:342401/1‑150 (MQ=255) taacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGATTACAGCTTTaac > 2:599067/1‑150 (MQ=255) caaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTaacaac < 3:81299/150‑1 (MQ=255) aaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGtt < 1:680484/68‑1 (MQ=255) aaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGtt > 2:680484/1‑68 (MQ=255) gcaacTACGGCGGCAACAGGAGTTGGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAAGACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATc < 3:281273/149‑1 (MQ=255) gcggcAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCaataataat < 4:52581/150‑1 (MQ=255) aaaaGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGa > 3:297665/1‑150 (MQ=255) aaaGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAg < 2:560556/150‑1 (MQ=255) | GAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAG > NC_000913_3_KO_pgi/3226046‑3226335 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |