breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 668,742 C→G E99Q (GAA→CAA)  rsfS ← ribosomal silencing factor
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,513,351 C→A L104M (CTG→ATG)  nupC → nucleoside (except guanosine) transporter
RA 3,440,135 A→C S299A (TCT→GCT)  rpoA ← RNA polymerase, alpha subunit
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC 4,233,757 +46 bp intergenic (‑524/‑583) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
JC 4,233,840 +46 bp intergenic (‑607/‑500) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
RA 4,294,815 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_KO_pgi 1403767 1408659 4893 13 [12] [12] 13 [abgA]–[ydaN] [abgA],abgR,mcaS,smrA,ydaM,[ydaN]
* * ÷ NC_000913_3_KO_pgi 1411908 1417222 5315 13 [11] [11] 13 [ttcA]–[recE] [ttcA],intR,ydaQ,ydaC,ralR,recT,[recE]
* * ÷ NC_000913_3_KO_pgi 1417227 1419921 2695 13 [12] [12] 13 [recE]–[racR] [recE],racC,ydaE,kilR,sieB,ydaF,ydaG,[racR]
* * ÷ NC_000913_3_KO_pgi 1419927 1426674 6748 13 [12] [12] 13 [racR]–[tmpR] [racR],ydaS,ydaT,ydaU,ydaV,ydaW,rzpR,rzoR,trkG,ynaK,ydaY,[tmpR]
* * ÷ NC_000913_3_KO_pgi 3423757–3424234 3424561–3424239 6–805 13 [12] [12] 13 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 1207790 =4 (0.100)32 (0.930) 27/248 0.4 85.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_KO_pgi 1209619 = 7 (0.200)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_KO_pgi = 12078054 (0.100)33 (0.960) 32/248 0.2 86.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_KO_pgi = 1209602 7 (0.200)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_KO_pgi = 13955419 (0.230)43 (1.110) 39/280 0.1 82.7% coding (382/1032 nt) ynaI mechanosensitive channel protein, very small conductance
?NC_000913_3_KO_pgi 1427777 = 9 (0.230)coding (950/981 nt) insH1 IS5 transposase and trans‑activator