New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 1086010 =68 (1.340)6 (0.130) 6/268 6.1 8.2% coding (1019/1065 nt) phoH ATP‑binding protein; putative PhoH family P‑loop ATPase
?NC_000913_3_KO_pgi 1086040 = 71 (1.490)coding (1049/1065 nt) phoH ATP‑binding protein; putative PhoH family P‑loop ATPase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGC  >  NC_000913_3_KO_pgi/1085865‑1086158
                                                                                                                                                  |                                                                                                                                                    
ctgggggACACCGTGACGGGTATCGTCAACGGTGATATAACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTTGAAGAAGATGAAACGGTTGGGATCGTCCGGTTCGGTAAAGAGGCCTGCGt                                                                                                                                                   <  2:293715/146‑1 (MQ=255)
   ggggAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACg                                                                                                                                                >  1:8198/1‑149 (MQ=255)
   ggggAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTAGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGAGTACg                                                                                                                                                >  2:793726/1‑149 (MQ=255)
        ggaCGTGACGGTTAGCGTCAACGGGTATATCACGTATTGCGATTTTCCTCGGGGCGTGTCCTTCAGACTGCGGGGCGCGCAGCAACGTGTCGAAGAAGATGCAATGGTGTGTATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCgg                                                                                                                                           <  2:324/147‑1 (MQ=255)
         aaCGTGACGGTTATCCTCAACTATGATATCACGCAATGCGATTTGCCTCGCGGCGTGTTCTCCGGATTAAGTGACGCGCTGAAACGTTTCGAAGAAGATGAAAGGGTTGGGATCGTCCGGCTCGCTAACGAGGACTGCGTACGTTCGGc                                                                                                                                          >  2:107728/1‑149 (MQ=255)
                    tATCGTCAACGGTAATATCACGCCCTGTGTTTGGCCTTGCGACGTTATGGGTGTTTTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAAc                                                                                                                               <  1:658700/149‑1 (MQ=255)
                     gtCGTCAACCGTGATATCACCCAATGCGATTTGCCTCGCGGCGTGGGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACg                                                                                                                              <  1:80520/148‑1 (MQ=255)
                          cAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGc                                                                                                                         >  2:652877/1‑149 (MQ=255)
                              ggTGATATCACGCAATGCGATTTGCCTCGCGGGGTGTGCTCTGGATTAAGGTGCTCGCTGGAACTTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGTGGACTGCGTACTTTCGGCTCTTTGCCAACGTACGCTgta                                                                                                                     <  2:109925/149‑3 (MQ=255)
                               gTGATATCACGCACTGCGCTGTGCCTTGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGCAGCCCTTTGGACCATCAGCTGGCAGTATTTGGACTGCGTACGTTCCGCACTTTGCCAACGTACGCTGCAt                                                                                                                    <  1:666168/149‑1 (MQ=255)
                                    atCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCCCTTAGCCCGCGTACGCTGCATGCCTa                                                                                                               >  2:224166/1‑149 (MQ=255)
                                    atCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTa                                                                                                               >  1:243440/1‑149 (MQ=255)
                                     tCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGGCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTAc                                                                                                              >  1:243589/1‑149 (MQ=255)
                                      cACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGCTCGTCCGGTTCGGTAAAGAGGACTGCGTAGGTTAGGCACTTTGCCAACGGTCGCTGCGTGCCTACa                                                                                                             >  2:127150/1‑149 (MQ=255)
                                       aCGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCCACGTACGCTGCATGCCTACAg                                                                                                            >  2:885871/1‑149 (MQ=255)
                                        cGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGCTTCAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCGGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGt                                                                                                           >  2:418134/1‑149 (MQ=255)
                                        cGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGAAACCGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGt                                                                                                           >  2:418123/1‑149 (MQ=255)
                                           aaTGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTaa                                                                                                        >  1:249592/1‑149 (MQ=255)
                                                     gCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATc                                                                                              >  2:80002/1‑149 (MQ=255)
                                                      ccTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCg                                                                                             <  2:601887/149‑1 (MQ=255)
                                                       cTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTTCCAACGTACGCTGCATGCCTACAGTTAAGTGTATTATCgg                                                                                            >  2:863356/1‑149 (MQ=255)
                                                        tCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGt                                                                                           >  2:67976/1‑149 (MQ=255)
                                                                  tgCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCg                                                                                 >  2:677860/1‑149 (MQ=255)
                                                                   gCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCgg                                                                                >  1:581459/1‑149 (MQ=255)
                                                                    cTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCggg                                                                               <  1:168742/149‑1 (MQ=255)
                                                                    cTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGACGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCggg                                                                               >  1:793008/1‑149 (MQ=255)
                                                                     tCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGTCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGc                                                                              >  2:512643/1‑149 (MQ=255)
                                                                     tCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGc                                                                              >  1:49940/1‑149 (MQ=255)
                                                                      ccGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCg                                                                             >  1:480023/1‑149 (MQ=255)
                                                                                tGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGGCCTGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGAGCGAAACgggagt                                                                   >  1:714785/1‑146 (MQ=255)
                                                                                 gACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCttt                                                                  <  1:677860/149‑1 (MQ=255)
                                                                                  aCGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTACGTGTGTTATCGGTGCAGAGCCCGGGCGCACCGTGCGTTg                                                                 >  2:831657/1‑149 (MQ=255)
                                                                                   cgcgCTGGAACGTCTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGt                                                                <  1:604825/149‑1 (MQ=255)
                                                                                     cgcTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGttt                                                              <  1:80002/149‑1 (MQ=255)
                                                                                      gcTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGtttt                                                             <  1:224166/149‑1 (MQ=255)
                                                                                             cGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGtt                                                      <  1:289135/149‑1 (MQ=255)
                                                                                                ttCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTTGGTGTTTAt                                                   >  1:105405/1‑149 (MQ=255)
                                                                                                     agaagaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCg                                              <  1:513546/149‑1 (MQ=255)
                                                                                                     agaagaTGAAACGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCg                                              <  2:552331/149‑1 (MQ=255)
                                                                                                      gaagaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGt                                                                                           <  2:418076/103‑1 (MQ=255)
                                                                                                      gaagaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGt                                                                                           >  1:418076/1‑103 (MQ=255)
                                                                                                      gaagaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCgg                                             >  1:469799/1‑149 (MQ=255)
                                                                                                       aagaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGACGAGCCCCGGGGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGa                                            >  1:390893/1‑149 (MQ=255)
                                                                                                        agaTGAAATGGTTGGGATCGTCCGGTTCGGTACAGAGGACTGCGTACGGTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTACGTGGCTTATCGGTGCAGAGCCCGGGCGAACCGGGCTCGGTTTTGGGGGTTTATGCCCGGAc                                           >  2:457609/1‑149 (MQ=255)
                                                                                                         gaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCg                                              <  2:124343/145‑1 (MQ=255)
                                                                                                         gaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCg                                              >  1:124343/1‑145 (MQ=255)
                                                                                                         gaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACt                                          >  2:810705/1‑149 (MQ=255)
                                                                                                          cggAAAGGGGTGGGATGGTCCGGTTCGGCAAACAACGCTGCTGGTGGCGGTCAGTTTTCCAACGTACGCTGCATGCCTACAGTTGAGTGTGTTAGCGGTGCAGGGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTa                                         <  2:868063/147‑1 (MQ=255)
                                                                                                          aTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTa                                         <  1:810705/149‑1 (MQ=255)
                                                                                                          aTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTATTTTGGGTGTTTATGCCCGGACTa                                         <  1:785109/149‑1 (MQ=255)
                                                                                                            gAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAgc                                       <  1:652877/149‑1 (MQ=255)
                                                                                                               aTGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAgtgt                                                                                                    <  2:700364/85‑1 (MQ=255)
                                                                                                               aTGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAgtgt                                                                                                    >  1:700364/1‑85 (MQ=255)
                                                                                                                   ttGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCttttt                                >  2:599150/1‑149 (MQ=255)
                                                                                                                       gATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTgg                            >  2:537651/1‑148 (MQ=255)
                                                                                                                       gATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGa                            >  2:241084/1‑149 (MQ=255)
                                                                                                                          cGTCCGGTGCGGGAAAGAGGACTGCGGACGTTCGGCACTTTGCCAACGTAAGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAaac                         <  1:107728/149‑1 (MQ=255)
                                                                                                                            tCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAaacaa                       <  1:599150/149‑1 (MQ=255)
                                                                                                                                gTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATg                                                  <  2:610257/118‑1 (MQ=255)
                                                                                                                                gTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATg                                                  >  1:610257/1‑118 (MQ=255)
                                                                                                                                gTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCAt                   <  2:560639/149‑1 (MQ=255)
                                                                                                                                 ttCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGCTAAGGGTGGTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTGTGCCCGGGCTGGCGCTTGTTCTGAAACAACCAta                  >  1:302875/1‑148 (MQ=255)
                                                                                                                                    cGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTCAAGTGTGTTATCGGTGCAGAGCCCGGGCTAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCAtttt                <  2:788653/149‑1 (MQ=255)
                                                                                                                                      gTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCg                                                                        <  2:458921/91‑1 (MQ=255)
                                                                                                                                      gTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCg                                                                        >  1:458921/1‑91 (MQ=255)
                                                                                                                                       tAAAGAGGACTGCGTACGTTCGGCACTTGGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCATAGCCCGGGCGAACCGGGCTTGGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAACCAACCATTTTTAt             <  2:422698/149‑1 (MQ=255)
                                                                                                                                           gagGTCTGCGTACGTTCGGCTCTTTGCCAACGTTCGCTGCAGGTCTACAGTTAAGTGTGTTATCGGTGCAGAGCTCGGGCGAACCGGGTTTTGTTTTGGGTGTTTTTGCCCGGACTTGCGCTTTTTCTGAAACAACCATTTTTACTTGc         <  2:899044/149‑1 (MQ=255)
                                                                                                                                              gACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTCTTTGcccc      >  2:455353/1‑149 (MQ=255)
                                                                                                                                                  gCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCctggc  >  1:25178/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGC  >  NC_000913_3_KO_pgi/1085865‑1086158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.