New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 1086010 =68 (1.340)6 (0.130) 6/268 6.1 8.2% coding (1019/1065 nt) phoH ATP‑binding protein; putative PhoH family P‑loop ATPase
?NC_000913_3_KO_pgi 1086040 = 71 (1.490)coding (1049/1065 nt) phoH ATP‑binding protein; putative PhoH family P‑loop ATPase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTT  >  NC_000913_3_KO_pgi/1085895‑1086187
                                                                                                                                                  |                                                                                                                                                   
ggTGATATCACGCAATGCGATTTGCCTCGCGGGGTGTGCTCTGGATTAAGGTGCTCGCTGGAACTTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGTGGACTGCGTACTTTCGGCTCTTTGCCAACGTACGCTgta                                                                                                                                                  <  2:109925/149‑3 (MQ=255)
 gTGATATCACGCACTGCGCTGTGCCTTGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGCAGCCCTTTGGACCATCAGCTGGCAGTATTTGGACTGCGTACGTTCCGCACTTTGCCAACGTACGCTGCAt                                                                                                                                                 <  1:666168/149‑1 (MQ=255)
      atCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCCCTTAGCCCGCGTACGCTGCATGCCTa                                                                                                                                            >  2:224166/1‑149 (MQ=255)
      atCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTa                                                                                                                                            >  1:243440/1‑149 (MQ=255)
       tCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGGCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTAc                                                                                                                                           >  1:243589/1‑149 (MQ=255)
        cACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGCTCGTCCGGTTCGGTAAAGAGGACTGCGTAGGTTAGGCACTTTGCCAACGGTCGCTGCGTGCCTACa                                                                                                                                          >  2:127150/1‑149 (MQ=255)
         aCGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCCACGTACGCTGCATGCCTACAg                                                                                                                                         >  2:885871/1‑149 (MQ=255)
          cGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGCTTCAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCGGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGt                                                                                                                                        >  2:418134/1‑149 (MQ=255)
          cGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGAAACCGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGt                                                                                                                                        >  2:418123/1‑149 (MQ=255)
             aaTGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTaa                                                                                                                                     >  1:249592/1‑149 (MQ=255)
                       gCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATc                                                                                                                           >  2:80002/1‑149 (MQ=255)
                        ccTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCg                                                                                                                          <  2:601887/149‑1 (MQ=255)
                         cTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTTCCAACGTACGCTGCATGCCTACAGTTAAGTGTATTATCgg                                                                                                                         >  2:863356/1‑149 (MQ=255)
                          tCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGt                                                                                                                        >  2:67976/1‑149 (MQ=255)
                                    tgCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCg                                                                                                              >  2:677860/1‑149 (MQ=255)
                                     gCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCgg                                                                                                             >  1:581459/1‑149 (MQ=255)
                                      cTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCggg                                                                                                            <  1:168742/149‑1 (MQ=255)
                                      cTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGACGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCggg                                                                                                            >  1:793008/1‑149 (MQ=255)
                                       tCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGTCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGc                                                                                                           >  2:512643/1‑149 (MQ=255)
                                       tCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGc                                                                                                           >  1:49940/1‑149 (MQ=255)
                                        ccGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCg                                                                                                          >  1:480023/1‑149 (MQ=255)
                                                  tGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGGCCTGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGAGCGAAACgggagt                                                                                                >  1:714785/1‑146 (MQ=255)
                                                   gACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCttt                                                                                               <  1:677860/149‑1 (MQ=255)
                                                    aCGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTACGTGTGTTATCGGTGCAGAGCCCGGGCGCACCGTGCGTTg                                                                                              >  2:831657/1‑149 (MQ=255)
                                                     cgcgCTGGAACGTCTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGt                                                                                             <  1:604825/149‑1 (MQ=255)
                                                       cgcTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGttt                                                                                           <  1:80002/149‑1 (MQ=255)
                                                        gcTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGtttt                                                                                          <  1:224166/149‑1 (MQ=255)
                                                               cGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGtt                                                                                   <  1:289135/149‑1 (MQ=255)
                                                                  ttCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTTGGTGTTTAt                                                                                >  1:105405/1‑149 (MQ=255)
                                                                       agaagaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCg                                                                           <  1:513546/149‑1 (MQ=255)
                                                                       agaagaTGAAACGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCg                                                                           <  2:552331/149‑1 (MQ=255)
                                                                        gaagaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGt                                                                                                                        >  1:418076/1‑103 (MQ=255)
                                                                        gaagaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGt                                                                                                                        <  2:418076/103‑1 (MQ=255)
                                                                        gaagaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCgg                                                                          >  1:469799/1‑149 (MQ=255)
                                                                         aagaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGACGAGCCCCGGGGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGa                                                                         >  1:390893/1‑149 (MQ=255)
                                                                          agaTGAAATGGTTGGGATCGTCCGGTTCGGTACAGAGGACTGCGTACGGTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTACGTGGCTTATCGGTGCAGAGCCCGGGCGAACCGGGCTCGGTTTTGGGGGTTTATGCCCGGAc                                                                        >  2:457609/1‑149 (MQ=255)
                                                                           gaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCg                                                                           >  1:124343/1‑145 (MQ=255)
                                                                           gaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCg                                                                           <  2:124343/145‑1 (MQ=255)
                                                                           gaTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACt                                                                       >  2:810705/1‑149 (MQ=255)
                                                                            cggAAAGGGGTGGGATGGTCCGGTTCGGCAAACAACGCTGCTGGTGGCGGTCAGTTTTCCAACGTACGCTGCATGCCTACAGTTGAGTGTGTTAGCGGTGCAGGGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTa                                                                      <  2:868063/147‑1 (MQ=255)
                                                                            aTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTa                                                                      <  1:810705/149‑1 (MQ=255)
                                                                            aTGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTATTTTGGGTGTTTATGCCCGGACTa                                                                      <  1:785109/149‑1 (MQ=255)
                                                                              gAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAgc                                                                    <  1:652877/149‑1 (MQ=255)
                                                                                 aTGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAgtgt                                                                                                                                 <  2:700364/85‑1 (MQ=255)
                                                                                 aTGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAgtgt                                                                                                                                 >  1:700364/1‑85 (MQ=255)
                                                                                     ttGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCttttt                                                             >  2:599150/1‑149 (MQ=255)
                                                                                         gATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTgg                                                         >  2:537651/1‑148 (MQ=255)
                                                                                         gATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGa                                                         >  2:241084/1‑149 (MQ=255)
                                                                                            cGTCCGGTGCGGGAAAGAGGACTGCGGACGTTCGGCACTTTGCCAACGTAAGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAaac                                                      <  1:107728/149‑1 (MQ=255)
                                                                                              tCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAaacaa                                                    <  1:599150/149‑1 (MQ=255)
                                                                                                  gTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATg                                                                               >  1:610257/1‑118 (MQ=255)
                                                                                                  gTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATg                                                                               <  2:610257/118‑1 (MQ=255)
                                                                                                  gTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCAt                                                <  2:560639/149‑1 (MQ=255)
                                                                                                   ttCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGCTAAGGGTGGTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTGTGCCCGGGCTGGCGCTTGTTCTGAAACAACCAta                                               >  1:302875/1‑148 (MQ=255)
                                                                                                      cGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTCAAGTGTGTTATCGGTGCAGAGCCCGGGCTAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCAtttt                                             <  2:788653/149‑1 (MQ=255)
                                                                                                        gTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCg                                                                                                     <  2:458921/91‑1 (MQ=255)
                                                                                                        gTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCg                                                                                                     >  1:458921/1‑91 (MQ=255)
                                                                                                         tAAAGAGGACTGCGTACGTTCGGCACTTGGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCATAGCCCGGGCGAACCGGGCTTGGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAACCAACCATTTTTAt                                          <  2:422698/149‑1 (MQ=255)
                                                                                                             gagGTCTGCGTACGTTCGGCTCTTTGCCAACGTTCGCTGCAGGTCTACAGTTAAGTGTGTTATCGGTGCAGAGCTCGGGCGAACCGGGTTTTGTTTTGGGTGTTTTTGCCCGGACTTGCGCTTTTTCTGAAACAACCATTTTTACTTGc                                      <  2:899044/149‑1 (MQ=255)
                                                                                                                gACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTCTTTGcccc                                   >  2:455353/1‑149 (MQ=255)
                                                                                                                    gCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCctggc                               >  1:25178/1‑149 (MQ=255)
                                                                                                                           ttCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGtttt                                                                                          <  2:275993/83‑1 (MQ=255)
                                                                                                                           ttCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGtttt                                                                                          >  1:275993/1‑83 (MQ=255)
                                                                                                                           ttCGGCACGTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGaa                        <  1:98046/149‑1 (MQ=255)
                                                                                                                              ggCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAgtg                     >  1:853666/1‑149 (MQ=255)
                                                                                                                                cACTTTGCGAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTa                   <  2:136397/149‑1 (MQ=255)
                                                                                                                                cACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTa                   <  2:853666/149‑1 (MQ=255)
                                                                                                                                cACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGCGGAGTGTa                   >  1:532787/1‑149 (MQ=255)
                                                                                                                                        cAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGCGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATc           >  2:594555/1‑149 (MQ=255)
                                                                                                                                        cAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATc           <  2:49940/149‑1 (MQ=255)
                                                                                                                                        cAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGACCATGGCGGGTGAAGTGTACGCTCagc           >  1:842556/1‑147 (MQ=255)
                                                                                                                                           cGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCtg        <  1:763218/149‑1 (MQ=255)
                                                                                                                                                 cTGCATGCCTACAGTTACGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTGTATGCCCGGACTAGCGCTTTTTCCGTCACAAGCATTTTTTTTTGCCCCTGTTTGGTGACGTTTACGCCAATCCTGTGGcct  >  2:164395/1‑147 (MQ=255)
                                                                                                                                                 cTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCtt  <  1:75077/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTT  >  NC_000913_3_KO_pgi/1085895‑1086187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.