breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 202,398 C→T R100C (CGC→TGC)  fabZ → (3R)‑hydroxymyristol acyl carrier protein dehydratase
MC JC 257,908 Δ776 bp [crl] [crl]
RA 1,398,976 C→A R184L (CGC→CTC)  fnr ← oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor
RA 1,529,185 C→T pseudogene (1284/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,203 C→A pseudogene (1302/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,218 C→T pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
MC JC 1,678,015 Δ73 bp intergenic (‑112/‑340) pntA ← / → ydgH pyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein
RA 1,731,621 G→T M845I (ATG→ATT lhr → putative ATP‑dependent helicase
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,867,401 Δ1 bp coding (151/993 nt) rpoS ← RNA polymerase, sigma S (sigma 38) factor
RA 3,440,176 G→A T285I (ACC→ATC)  rpoA ← RNA polymerase, alpha subunit
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,234,667 Δ1 bp intergenic (‑1434/‑499) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
RA 4,295,641 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_pgi 731365–731470 731718–731478 9–354 80 [75] [75] 76 rhsC Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
* * ÷ NC_000913_3_pae_pgi 3423698–3424600 3424600 1–903 78 [75] [75] 76 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi 1207790 =10 (0.070)101 (0.820) 77/230 0.7 90.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_pae_pgi 1209619 = 13 (0.110)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_pae_pgi = 120780510 (0.070)101 (0.820) 69/230 1.2 90.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_pae_pgi = 1209602 13 (0.110)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_pae_pgi = 12994980 (0.000)169 (1.240) 120/254 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein