New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi = 117410631 (1.610)4 (0.210) 4/282 2.1 12.7% coding (1060/1074 nt) ycfT inner membrane protein
?NC_000913_3_vch_pgi = 1174164 25 (1.330)coding (1002/1074 nt) ycfT inner membrane protein
Rejected: Frequency below/above cutoff threshold.

TCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_vch_pgi/1174014‑1174106
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aactTTCACAGCGTGCATTCAGCGATCTGTTGTTCTCCCCGCCTTCACTGCCCGCTGCCGTCAGTTACTCCCGCTAAGCCATC  <  NC_000913_3_vch_pgi/1174164‑1174086
                                                                                                                                                                            
TCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTTTCACAGCGTGCAGTCAGCGATCTGTTGTTCTCCCCGCCTTCACTGCCCGCTGCCG                         >  1:281971/1‑149
          GCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTTTCACAGCGTGCATTCAGCGATCTGTTGTTCTCCCCGCCTTCACTGCCCGCTGCCGTCAGTTACTC               >  1:254684/1‑149
                   TTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTTTCACAGCGTGCATTCAGCGATCTGTTGTTCTCCCCGCCTTCACTGCCCGCTGCCGTCAGTTACTCCCGCTAAGC      <  2:254684/149‑1
                       TTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTTTCACAGCGTGCATTCAGCGATCTGTTGTTCTCCCCGCCTTCACTGCCCGCTGCCGTCAGTTACTCCCGCTAAGCCATC  <  2:281971/149‑1
                                                                                                                                                                            
TCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_vch_pgi/1174014‑1174106
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aactTTCACAGCGTGCATTCAGCGATCTGTTGTTCTCCCCGCCTTCACTGCCCGCTGCCGTCAGTTACTCCCGCTAAGCCATC  <  NC_000913_3_vch_pgi/1174164‑1174086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.