New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi = 117410631 (1.610)4 (0.210) 4/282 2.1 12.7% coding (1060/1074 nt) ycfT inner membrane protein
?NC_000913_3_vch_pgi = 1174164 25 (1.330)coding (1002/1074 nt) ycfT inner membrane protein
Rejected: Frequency below/above cutoff threshold.

TAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGC  >  NC_000913_3_vch_pgi/1174021‑1174291
                                                                                                                                               |                                                                                                                               
tAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGttt                                                                                                                            >  2:14560/1‑149 (MQ=255)
tAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGttt                                                                                                                            >  2:13376/1‑149 (MQ=255)
   gCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCt                                                                                                                         >  1:157846/1‑149 (MQ=255)
   gCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCt                                                                                                                         <  1:206337/149‑1 (MQ=255)
      aTGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTAc                                                                                                                      >  1:256150/1‑149 (MQ=255)
         cggtgcATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCGGGGGCGATGGCTTAGCGTGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAg                                                                                                                   <  2:20301/144‑1 (MQ=255)
            ttCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCaa                                                                                                                <  2:307367/149‑1 (MQ=255)
            ttCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCaa                                                                                                                >  1:34005/1‑149 (MQ=255)
                ttATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGcgc                                                                                                            >  2:81824/1‑147 (MQ=255)
                         acgcaCATGGTTTAGCAAAGCAAATCCCCGCGGCTGGGGGGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTa                                                                                                   <  2:210244/145‑1 (MQ=255)
                           agACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTCAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACa                                                                                                 <  1:90785/148‑1 (MQ=255)
                             aCATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCGGTGGAGGCGGGGAGAAAAACAGATCGCTGAATGCACGCGGTGAAAGTTTTCTTACCAGCAAGACCGCAACAGTGCAGa                                                                                               >  1:29089/1‑149 (MQ=255)
                              cATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTTCAGGCGGGGAGAACAACAGATCGCTGAATGCACCCAGTGAAAGTGTTCTTACCAGCAAGCCCACACCAGTACAGAt                                                                                              >  2:329938/1‑149 (MQ=255)
                                 ggTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGaa                                                                                           <  1:13376/149‑1 (MQ=255)
                                 ggTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGaa                                                                                           <  2:185562/149‑1 (MQ=255)
                                         aaaTTGGATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGa                                                                                   <  1:155707/149‑1 (MQ=255)
                                              gAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCTCCCTGTGACCGTTCACGGATGAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAg                                                                              <  1:335778/149‑1 (MQ=255)
                                               aaTCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAgg                                                                             >  1:269771/1‑149 (MQ=255)
                                               aaTCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAgg                                                                             <  1:175889/149‑1 (MQ=255)
                                                     cccGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATaaa                                                                       <  2:34005/149‑1 (MQ=255)
                                                                 gATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAagttactcccgctaagccatcgccccagccggggcgattcaatttgctaaaccatgtcgattacggataa                                                           <  2:281971‑M2/149‑71 (MQ=255)
                                                                     gCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAagttactcccgctaagccatcgccccagccggggcgattcaatttgctaaaccatgtcgattacggataatgaa                                                       <  2:254684‑M2/149‑75 (MQ=255)
                                                                       ttAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGAACGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGAACAGATGAACAAACTGACAAAGGCATAAACAAGCAGGACAGTAAGtt                                                     >  2:261346/1‑149 (MQ=255)
                                                                          gCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAGTCCATATCCTGATGGATAAACCAGCAGGAGTGTCAGTTCGa                                                  <  1:81824/149‑1 (MQ=255)
                                                                              gAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAagttactcccgctaagccatcgccccagccggggcgattcaatttgctaaaccatgtcgattacggataatgaacagcattgc                                              >  1:254684‑M2/1‑66 (MQ=255)
                                                                                        cGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGACTGCACGCTGTGAAagttactcccgctaagccatcgccccagccggggcgattcaatttgctaaaccatgtcgattacggataatgaacagcattgcttatcagaga                                    >  1:281971‑M2/1‑56 (MQ=255)
                                                                                                ggCAGGGAAGGCAGGGAGAACAACAGCTCGCCGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTAAAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGtgctgc                            <  1:19711/149‑1 (MQ=255)
                                                                                                                     aacaGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGGGCAAGCAGAGt       >  2:242886/1‑149 (MQ=255)
                                                                                                                          aTCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGGTAAGc  >  1:114807/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                               
TAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGC  >  NC_000913_3_vch_pgi/1174021‑1174291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.