New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_vch_pgi	= 3510605	14 (0.730)	3 (0.160)	3/286	2.6	17.8%	coding (372/1164 nt)	yhfX	putative pyridoxal 5'‑phosphate binding protein
	?	NC_000913_3_vch_pgi	= 3510636	14 (0.740)					coding (341/1164 nt)	yhfX	putative pyridoxal 5'‑phosphate binding protein
Rejected: Frequency below/above cutoff threshold.

GCCCGGATAAAGAAAATCATCGTCGCTATAAACTTTAAGCAGCACAGACTGGATTCGCCCGGCCTTCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_vch_pgi/3510491‑3510605 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgCTGACGCCGTTGAACAGGGCACCGACGTCATCACCGTGTTTACTCTCG  <  NC_000913_3_vch_pgi/3510636‑3510589                                                                                                                                                                     gcgCGGATAAAGAAAATCATCGGCGCGATAAACTTTAAGCAGCACAGACTGGAGTCGCCCGGCCTTCACCGCCGCCGCAGAATCTTCCTGCGCTTTGTCGAGAGTAAACACGGTGATGACGCCGTTGAACAGGGCACCGACGTCATCAC                <  2:68648/146‑1                    TCGTCGCTATAAACTTTAAGCAGCACAGACTGGATTCGCCCGGCCTTCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTGCTGACGCCGTTGAACAGGGCACCGACGTCATCACCGTGTTTACTCTCG  >  1:17507/1‑144                    TCGTCGCTATAAACTTTAAGCAGCACAGACTGGATTCGCCCGGCCTTCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTGCTGACGCCGTTGAACAGGGCACCGACGTCATCACCGTGTTTACTCTCG  <  2:17507/144‑1                                                                                                                                                                     GCCCGGATAAAGAAAATCATCGTCGCTATAAACTTTAAGCAGCACAGACTGGATTCGCCCGGCCTTCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_vch_pgi/3510491‑3510605 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgCTGACGCCGTTGAACAGGGCACCGACGTCATCACCGTGTTTACTCTCG  <  NC_000913_3_vch_pgi/3510636‑3510589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.