New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_vch_pgi	= 3510605	14 (0.730)	3 (0.160)	3/286	2.6	17.8%	coding (372/1164 nt)	yhfX	putative pyridoxal 5'‑phosphate binding protein
	?	NC_000913_3_vch_pgi	= 3510636	14 (0.740)					coding (341/1164 nt)	yhfX	putative pyridoxal 5'‑phosphate binding protein
Rejected: Frequency below/above cutoff threshold.

GATAAAGAAAATCATCGTCGCTATAAACTTTAAGCAGCACAGACTGGATTCGCCCGGCCTTCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTGATGACGTCGGTGCCCTGTTCAACGGCGTCAGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCAC  >  NC_000913_3_vch_pgi/3510496‑3510776                                                                                                                                             |                                                                                                                                             gATAAAGAAAATCATCGTCGCTATAAACTTTAAGCAGCACAGACTGGATTCGCCCGGCCTTCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTGATGACGTCGGTGCCCTGTTCAACGGCGTCAGCAACCtga                                                                                                                                      >  1:38760/1‑149 (MQ=255)       gAAAATCATCGTCGCTATAAACTTTAAACAGCACAGACTGGATTCGCCCGACCTTCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTGCAGACGTCGGTGCCCTGTTCAACGGCGGACGCAACCAGGTAACaa                                                                                                                                >  2:97100/1‑149 (MQ=255)              atcGTCGCTATAAACTTTAAGCAGCACAGACTGGATTCGCCCGGCCTTCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTGATGACGTCGGTGCCCTGTTCAACGGCGTCAGCAACCTGATGACAAGGGAttt                                                                                                                         <  1:46104/149‑1 (MQ=255)                            cTTTGAGCAGCACAGACGTGATTGGCCCGGTCTTCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTAGAGAGTACACACTGTGATGACGTGGGTGCCCTGTTGAACGGCGCCATCAACCTGATGACAAGGGATTTGTACCAGATGcccc                                                                                                           <  2:80740/149‑1 (MQ=255)                            cTTTAATCAGCACAGACTGGATTCGCCCGGCCTTCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTGATGACGTCGGTGTCCTGTTCAACGGCGTCAGCAACCTGATGACAAGGGATTTGTACCAGATGcccc                                                                                                           <  1:222114/149‑1 (MQ=255)                                                    gCCCGGCCTTCACCGCCGCCGCAGAAACTTCCCGCGCTTTGACGAATGAAAACCCGTTTTTCTTGTCTATTCCATGGTCAACGGCGGCAGCAACCTGATGACAAGGGATTTGTACCACATGCCCCTCATGCGCCACAGGCAAACCAgcg                                                                                   >  2:72022/1‑149 (MQ=255)                                                            ttCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTGATGACGTCGGTGCCCTGTTCAACGGCGTCAGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAg                                                                                              >  1:114646/1‑130 (MQ=255)                                                            ttCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTGATGACGTCGGTGCCCTGTTCAACGGCGTCAGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAg                                                                                              <  2:114646/130‑1 (MQ=255)                                                            ttCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTGATGACGTCGGTGCCCTGTTCAACGGCGTCAGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAg                                                                                              <  2:114665/130‑1 (MQ=255)                                                            ttCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTGATGACGTCGGTGCCCTGTTCAACGGCGTCAGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAg                                                                                              >  1:114665/1‑130 (MQ=255)                                                            ttCACCGCCGCCGCAGAAACTTCAACCGCTTTGTCGAGAGTAAACACGGTGATGACGTCGGTGCCCCGTTCCACGGCGTCAGCAACCTTATGACCAGGGAGTTGTACCAGAGGCCCCTGACGCGCCACAGCCAAACCATCGCGGCACAt                                                                           >  2:143282/1‑149 (MQ=255)                                                                                            gTCGAGAGTAAACACGGTGATGACGTCGGTGCCCTGATCAACGGCGTCAGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCCGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACaa                                           >  1:31353/1‑149 (MQ=255)                                                                                              cGAGAGTAAACACGGTGATGACGTCGGTGCCCTGTTCAACGGCGTCAGcaccgtgtttactctcgacaaagcgcgggaagtttctgcggcggcggtgaaggccgggcgaatccagtctgtgctgcttaaagtttatagcgacga                                              >  1:17507‑M2/1‑48 (MQ=255)                                                                                              cGAGAGTAAACACGGTGATGACGTCGGTGCCCTGTTCAACGGCGTCAGcaccgtgtttactctcgacaaagcgcgggaagtttctgcggcggcggtgaaggccgggcgaatccagtctgtgctgcttaaagtttatagcgacga                                              <  2:17507‑M2/144‑97 (MQ=255)                                                                                                            gTGATGACGTCGGTGCCCTGTTCAACGGCGTCATcaccgtgtttactctcgacaaagcgcaggaagattctgcggcggcggtgaaggccgggcgactccagtctgtgctgcttaaagtttatcgcgccgatgattttctttatccgcgc                           <  2:68648‑M2/149‑116 (MQ=255)                                                                                                                           cccTGTTCAACGGCGTCAGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTc            <  1:136310/149‑1 (MQ=255)                                                                                                                                     cGGCGTCAGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCATACCAGCGCGGCGCATGACTCGCGCCCCTTTGTAATCCACCGCCCCAATGCCGCTGTAGCCTAATGCCCGCAATTTTTCCGCCAGCCAc  >  1:134029/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                             GATAAAGAAAATCATCGTCGCTATAAACTTTAAGCAGCACAGACTGGATTCGCCCGGCCTTCACCGCCGCCGCAGAAACTTCCCGCGCTTTGTCGAGAGTAAACACGGTGATGACGTCGGTGCCCTGTTCAACGGCGTCAGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCAC  >  NC_000913_3_vch_pgi/3510496‑3510776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.