New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 3548549	38 (0.770)	9 (0.220) +TTTTTTTTTAATGATACGGCGAC	8/236	4.2	22.5%	coding (1522/2085 nt)	malQ	4‑alpha‑glucanotransferase (amylomaltase)
	?	NC_000913_3_hsa_tpiA	3548573 =	36 (0.730)					coding (1498/2085 nt)	malQ	4‑alpha‑glucanotransferase (amylomaltase)
Rejected: Coverage evenness skew score above cutoff.

CCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGG  >  NC_000913_3_hsa_tpiA/3548403‑3548693                                                                                                                                                   |                                                                                                                                                         ccAGTAACCGCGGAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTAc                                                                                                                                                        <  1:353333/149‑1 (MQ=255) ccAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTAc                                                                                                                                                        >  1:28366/1‑149 (MQ=255)  cAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCTGCTCAcc                                                                                                                                                       >  1:283563/1‑149 (MQ=255)    gTAACCGCGCAGCGTTGGCAGGTCATGTGGCGCCGCAACCGCCATTGACTGCTCCGGTTACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGCGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGa                                                                                                                                                     <  2:155156/149‑1 (MQ=255)        ccacagaGCGTGGGCAGGTCATGTGTCGCCGAAACCGCCATGGACTTTGCCGAATACGCGTTCGGTGCACGGAACGTCTTCTCGTGGCCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAAt                                                                                                                                                 <  2:155292/143‑1 (MQ=255)        ccGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACGTTGTAAGGGTACACACCGCTGCTGCGCCGCTTACCGACAAt                                                                                                                                                 >  1:14712/1‑149 (MQ=255)         cgcgCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGGTACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCTAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAAtc                                                                                                                                                >  2:660047/1‑149 (MQ=255)         cgcgCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACAGCTGCAGCGCAGCTTACCGACAAtc                                                                                                                                                >  2:685031/1‑149 (MQ=255)               gCGTTGGCAGGTCATGTGGCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTAcc                                                                                                                                          <  1:685451/149‑1 (MQ=255)                     gCAGGTCATGTGTCGCCGCCACCGCCATCGACCGCTCCGCATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGTGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACcggtacg                                                                                                                                    <  1:294067/149‑1 (MQ=255)                        ggTGATGTGTCGCCGCCACCGCCATCGACTGCTCCGGATACGCTTTCGGGGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACcggtacgg‑‑‑‑t‑‑‑‑a                                                                                                                         <  2:82152/149‑1 (MQ=255)                         gTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTAc                                                                                                                                           >  2:249292/1‑138 (MQ=255)                         gTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTAc                                                                                                                                           <  1:249292/138‑1 (MQ=255)                            aTGTGTCGCCGCCACCGCCATCGACTGCTGCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCga                                                                                                                     <  2:323048/149‑1 (MQ=255)                            aTGTATCGCCCCAACCGCCATCGACTGCTCCGGATACCGTTGGGGTGCCCGGAACGTCTTCTCGTGGTCGTTTTCGAAACAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCga                                                                                                                     <  2:750196/149‑1 (MQ=255)                                 tCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAACTACAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACCATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGACCt                                                                                                                >  2:161557/1‑149 (MQ=255)                                 tCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCt                                                                                                                >  1:590980/1‑149 (MQ=255)                                     cgcAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCAc                                                                                                            <  1:366394/149‑1 (MQ=255)                                           cGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCaacta                                                                                                      >  1:621792/1‑146 (MQ=255)                                              cATCGAGTGTGCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACTCCCCGCTGCTGCGCAGCTTTTTTTTTTTCTCGACCCGGCGGC‑‑‑‑T‑‑‑‑ACCGAGATCTACACCAATCACCa                                                                                                   <  2:434535/149‑1 (MQ=255)                                              cATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGAGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACAAAATGATCAGACCCACCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATccccc                                                                                                   >  1:639063/1‑148 (MQ=255)                                                        tCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATg                                                                                         <  1:27873/149‑1 (MQ=255)                                                          cGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTCCTGCGCAGCTTACCGACAATCTCTACCGCTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGAc                                                                                       >  2:793721/1‑149 (MQ=255)                                                            gATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGt                                                                                     <  1:339310/149‑1 (MQ=255)                                                                   tttCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACAGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTAGGG‑‑‑‑T‑‑‑‑ACCGAGATCTTTAGCAATCACCATACTGCGATGACGTTTAGttt                                                                              >  2:776830/1‑149 (MQ=255)                                                                    ttCGGTGCAGGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTTTTTTTTTTCATGATACGGCGGC‑‑‑‑C‑‑‑‑ACCGAGATCTACACCAATCACCATACAGCGATGACGTTTACTTTc                                                                             <  2:825658/149‑1 (MQ=255)                                                                               gAACGTCTTCTCTTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACTGCTGCTGCGCAGCTT‑‑‑‑TTTTTTTTTAATGATACGGCGACC‑‑‑‑ACCGAGATCTACACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGaa                                                                  <  2:174396/149‑1 (MQ=255)                                                                                   gTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACATCGATGACGTTTACTTTCGAGTGCCAGAATCga                                                              <  1:405599/149‑1 (MQ=255)                                                                                    tcttctCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACAGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑AACGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAATGCGAGGATCgag                                                             >  1:449283/1‑149 (MQ=255)                                                                                    tcttcgCGTGTGCGTTTTCGAGATAGAGCATTTTGTAAGAGTACACACCGCTGCTGCGCAGTTT‑‑‑‑TTTTTTTTTAATGATACGGCGACC‑‑‑‑ACCGAGATCTACACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCgag                                                             <  2:852402/149‑1 (MQ=255)                                                                                      ttctCGTGCTCCTTCTCGAAATAGAGAACTTTCTAAGAGTACACACCCCTGCTTCGCAGCTT‑‑‑‑TTTTTTTTTAATGATACGG‑‑‑‑CGACCACCGAGATCTACACCAATCACCATAAAGCGATGACGTTTACTTTCGAGTGCCAGAATCGagag                                                           <  2:279329/149‑1 (MQ=255)                                                                                          cGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGa                                                       <  2:590980/149‑1 (MQ=255)                                                                                               tCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGatcatc                                                  <  1:660047/149‑1 (MQ=255)                                                                                                        aaaTAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGCGTGCCAGAATCGCGAGCACATCATCCCCCGGCTa                                         >  1:245137/1‑149 (MQ=255)                                                                                                        aaaTAGAGCACCTTGTAAGAGTACACACCGCTGCTGCGCAGCTTTCTGTTATTAATGACCGGTACGG‑‑‑‑C‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATa                                         <  2:496244/149‑1 (MQ=255)                                                                                                                  cTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATAc                               >  1:191912/1‑149 (MQ=255)                                                                                                                       tAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATTCGCGcc                          <  2:191912/149‑1 (MQ=255)                                                                                                                            gTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGAt                     <  1:776830/149‑1 (MQ=255)                                                                                                                                  acCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCg               >  1:90224/1‑149 (MQ=255)                                                                                                                                  acCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCg               >  1:621250/1‑149 (MQ=255)                                                                                                                                         ctgcGCAGCTCACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGcc                                                                      <  1:86878/87‑1 (MQ=255)                                                                                                                                         ctgcGCAGCTCACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGcc                                                                      >  2:86878/1‑87 (MQ=255)                                                                                                                                               aGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCCCCCTGATCTGCCGCCCCCCCATACgg  >  2:224628/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                         CCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGG  >  NC_000913_3_hsa_tpiA/3548403‑3548693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.