New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 3548549	38 (0.770)	9 (0.220) +TTTTTTTTTAATGATACGGCGAC	8/236	4.2	22.5%	coding (1522/2085 nt)	malQ	4‑alpha‑glucanotransferase (amylomaltase)
	?	NC_000913_3_hsa_tpiA	3548573 =	36 (0.730)					coding (1498/2085 nt)	malQ	4‑alpha‑glucanotransferase (amylomaltase)
Rejected: Coverage evenness skew score above cutoff.

GGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGGTATCCACCACAAACGCAGCATCGACAT  >  NC_000913_3_hsa_tpiA/3548426‑3548720                                                                                                                                                        |                                                                                                                                                        ggTGATGTGTCGCCGCCACCGCCATCGACTGCTCCGGATACGCTTTCGGGGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACcggtacgg‑‑‑‑t‑‑‑‑a                                                                                                                                                    <  2:82152/149‑1 (MQ=255)     aTGTGTCGCCGCCACCGCCATCGACTGCTGCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCga                                                                                                                                                <  2:323048/149‑1 (MQ=255)     aTGTATCGCCCCAACCGCCATCGACTGCTCCGGATACCGTTGGGGTGCCCGGAACGTCTTCTCGTGGTCGTTTTCGAAACAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCga                                                                                                                                                <  2:750196/149‑1 (MQ=255)          tCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAACTACAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACCATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGACCt                                                                                                                                           >  2:161557/1‑149 (MQ=255)          tCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCt                                                                                                                                           >  1:590980/1‑149 (MQ=255)              cgcAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCAc                                                                                                                                       <  1:366394/149‑1 (MQ=255)                    cGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCaacta                                                                                                                                 >  1:621792/1‑146 (MQ=255)                       cATCGAGTGTGCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACTCCCCGCTGCTGCGCAGCTTTTTTTTTTTCTCGACCCGGCGGC‑‑‑‑T‑‑‑‑ACCGAGATCTACACCAATCACCa                                                                                                                              <  2:434535/149‑1 (MQ=255)                       cATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGAGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACAAAATGATCAGACCCACCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATccccc                                                                                                                              >  1:639063/1‑148 (MQ=255)                                 tCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATg                                                                                                                    <  1:27873/149‑1 (MQ=255)                                   cGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTCCTGCGCAGCTTACCGACAATCTCTACCGCTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGAc                                                                                                                  >  2:793721/1‑149 (MQ=255)                                     gATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGt                                                                                                                <  1:339310/149‑1 (MQ=255)                                            tttCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACAGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTAGGG‑‑‑‑T‑‑‑‑ACCGAGATCTTTAGCAATCACCATACTGCGATGACGTTTAGttt                                                                                                         >  2:776830/1‑149 (MQ=255)                                             ttCGGTGCAGGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTTTTTTTTTTCATGATACGGCGGC‑‑‑‑C‑‑‑‑ACCGAGATCTACACCAATCACCATACAGCGATGACGTTTACTTTc                                                                                                        <  2:825658/149‑1 (MQ=255)                                                        gAACGTCTTCTCTTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACTGCTGCTGCGCAGCTT‑‑‑‑TTTTTTTTTAATGATACGGCGACC‑‑‑‑ACCGAGATCTACACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGaa                                                                                             <  2:174396/149‑1 (MQ=255)                                                            gTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACATCGATGACGTTTACTTTCGAGTGCCAGAATCga                                                                                         <  1:405599/149‑1 (MQ=255)                                                             tcttctCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACAGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑AACGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAATGCGAGGATCgag                                                                                        >  1:449283/1‑149 (MQ=255)                                                             tcttcgCGTGTGCGTTTTCGAGATAGAGCATTTTGTAAGAGTACACACCGCTGCTGCGCAGTTT‑‑‑‑TTTTTTTTTAATGATACGGCGACC‑‑‑‑ACCGAGATCTACACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCgag                                                                                        <  2:852402/149‑1 (MQ=255)                                                               ttctCGTGCTCCTTCTCGAAATAGAGAACTTTCTAAGAGTACACACCCCTGCTTCGCAGCTT‑‑‑‑TTTTTTTTTAATGATACGG‑‑‑‑CGACCACCGAGATCTACACCAATCACCATAAAGCGATGACGTTTACTTTCGAGTGCCAGAATCGagag                                                                                      <  2:279329/149‑1 (MQ=255)                                                                   cGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGa                                                                                  <  2:590980/149‑1 (MQ=255)                                                                        tCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGatcatc                                                                             <  1:660047/149‑1 (MQ=255)                                                                                 aaaTAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGCGTGCCAGAATCGCGAGCACATCATCCCCCGGCTa                                                                    >  1:245137/1‑149 (MQ=255)                                                                                 aaaTAGAGCACCTTGTAAGAGTACACACCGCTGCTGCGCAGCTTTCTGTTATTAATGACCGGTACGG‑‑‑‑C‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATa                                                                    <  2:496244/149‑1 (MQ=255)                                                                                           cTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATAc                                                          >  1:191912/1‑149 (MQ=255)                                                                                                tAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATTCGCGcc                                                     <  2:191912/149‑1 (MQ=255)                                                                                                     gTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGAt                                                <  1:776830/149‑1 (MQ=255)                                                                                                           acCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCg                                          >  1:90224/1‑149 (MQ=255)                                                                                                           acCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCg                                          >  1:621250/1‑149 (MQ=255)                                                                                                                  ctgcGCAGCTCACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGcc                                                                                                 >  2:86878/1‑87 (MQ=255)                                                                                                                  ctgcGCAGCTCACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGcc                                                                                                 <  1:86878/87‑1 (MQ=255)                                                                                                                        aGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCCCCCTGATCTGCCGCCCCCCCATACgg                             >  2:224628/1‑149 (MQ=255)                                                                                                                                   aaTCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGGTATCCACcaca                  >  1:342403/1‑149 (MQ=255)                                                                                                                                   aaTCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCCGATCTGCCGTCTCGCCATACGGTATCCACcaca                  >  1:27470/1‑149 (MQ=255)                                                                                                                                     tctcTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGGTATCCACCACaaa                >  1:783285/1‑149 (MQ=255)                                                                                                                                     tctcTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGGTATCCACCACaaa                >  1:542243/1‑149 (MQ=255)                                                                                                                                        ctACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGGTATCCACCACAAACgc             <  1:498153/149‑1 (MQ=255)                                                                                                                                         tACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCtcgt                                                <  2:228113/113‑4 (MQ=255)                                                                                                                                         tACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGAt                                                >  1:228113/1‑113 (MQ=255)                                                                                                                                         tACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTAACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGGTATCCACCACAAACgca            <  2:342403/149‑1 (MQ=255)                                                                                                                                                acgg‑‑‑‑t‑‑‑‑acCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGGTATCCACCACAAACGCAGCATCGa     <  1:224628/149‑1 (MQ=255)                                                                                                                                                   g‑‑‑‑t‑‑‑‑acCGATATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGGTATCCACCACAAACGCAGCATCGACAt  <  2:614239/149‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                        GGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGG‑‑‑‑T‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGGTATCCACCACAAACGCAGCATCGACAT  >  NC_000913_3_hsa_tpiA/3548426‑3548720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.