New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 3159421	18 (0.730)	4 (0.170)	4/272	2.8	17.7%	coding (1726/2220 nt)	ygiQ	Radical SAM superfamily protein
	?	NC_000913_3_hsa_tpiA	= 3159442	20 (0.850)					coding (1705/2220 nt)	ygiQ	Radical SAM superfamily protein
Rejected: Frequency below/above cutoff threshold.

CTTCGCCAGCGGGTTTTTCCCGGTGTAATACATGGTGGTTGAGTTCGCCAGCGGCGACGGATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGT  >  NC_000913_3_hsa_tpiA/3159287‑3159568                                                                                                                                       |                                                                                                                                                    cTTCGCCAGCGGGTTTTTCCCGGTGTAATACATGGTGGTTGAGTTCGCCAGCGGCGACGGATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGt                                                                                                                                       <  1:7028/149‑1 (MQ=255)            ggTTTTTCCCGGTGTAATACATGGTGGTTGAGTTGGCCAGCGGCGACGGATAGAAGTGCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAgatg                                                                                                                            >  1:376411‑M2/1‑145 (MQ=255)               ttttCCCGGTGTAATACATGGTGGTTGAGTTCGCCAGCGGCGACGGATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAgatgaga                                                                                                                         <  1:143400‑M2/149‑8 (MQ=255)                    ccGGTGTAATACATGGTGGTTGAGTTCGCCAGCGGCGACGGATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAggg                                                                                                                    <  2:46454/149‑1 (MQ=255)                    ccGGTGTAATACATGGTGGTTGAGTTCGCCAGCGGCGACGGATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAggg                                                                                                                    <  1:242122/149‑1 (MQ=255)                                      gctGAGGTCGGCAGCGGCGACGTATCGAAGTGCTGCCCCTGGGCGAGGCGGAAGCGGTGCTTTTTCAGCCATAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCttt                                                                                                  <  2:189360/147‑1 (MQ=255)                                          aGTTCGCCAGCGGCGACGGATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCtccgcgcaccccggtacgcgtgatgaagatat                                                                                                                     <  2:148038‑M1/126‑33 (MQ=255)                                          aGTTCGCCAGCGGCGACGGATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCtccgcgcaccccggtacgcgtgatgaagatat                                                                                                                     >  1:148038‑M1/1‑94 (MQ=255)                                           gTTCGCCAGCGGCGACGGATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCacgcgcaccccggtacgcgt                                                                                                                                 >  1:294117‑M1/1‑93 (MQ=255)                                           gTTCGCCAGCGGCGACGGATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCacgcgcaccccggtacgcgt                                                                                                                                 <  2:294117‑M1/113‑21 (MQ=255)                                              cGCCAGCGGCGACGGATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAAATACTGCTCTTTAcctgcct                                                                                          >  1:246116/1‑149 (MQ=255)                                                    cggcgACGGATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTg                                                                                    <  1:411974/149‑1 (MQ=255)                                                           ggATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGagtaagt                                                                             >  1:368379/1‑149 (MQ=255)                                                                            cTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCAAACAGCTCTTTaa                                                            >  2:81687/1‑149 (MQ=255)                                                                             tGGTCGAGGCGGCAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTaaa                                                           <  1:285981/149‑1 (MQ=255)                                                                                  gAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGt                                                      >  1:289035/1‑149 (MQ=255)                                                                                       ggAAGCTGTGCTTTTACAGCCACAGGGCCAGAGCCACCACATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGTGATCAGATACTGTTCTTTACTTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAg                                                 <  2:193157/149‑1 (MQ=255)                                                                                              gTGCTTTTTCAGCCACAGCGCCAGATTCACCTTATCTTCATCACGCGTACCGGGGTGCGCGGAGCTGAAATCGGGGATCAGATACTGCTTTTTACCGGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCa                                          >  1:16954/1‑149 (MQ=255)                                                                                               tGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCAt                                         >  2:207112/1‑149 (MQ=255)                                                                                                 cTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAAAAGCTCTTTAAAGCGGTCATGGCTGCCCATGc                                       >  1:154995/1‑149 (MQ=255)                                                                                                        aGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCtt                                >  1:231676/1‑149 (MQ=255)                                                                                                                 tccAGACTCACCATATCTTCATCACGCGTACCGGGGTGCGCTGAGATGAAATACGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTTCCCATGCCCGGCTTCATCATCtt                       <  1:398701/148‑1 (MQ=255)                                                                                                                             atatCTTCATCACGCGTACCGGGGTGCGCGGAgatgaagatatggtgaatctggcgctgtggctgaaaaagcaccgcttccgcctcgaccaggtgcagaacttctatccgtcgccgctggcgaact                                  <  2:148038‑M2/126‑95 (MQ=255)                                                                                                                             atatCTTCATCACGCGTACCGGGGTGCGCGGAgatgaagatatggtgaatctggcgctgtggctgaaaaagcaccgcttccgcctcgaccaggtgcagaacttctatccgtcgccgctggcgaact                                  >  1:148038‑M2/1‑32 (MQ=255)                                                                                                                                 tctcaTCtccgcgcaccccggtacgcgtgatgaagatatggtgaatctggcgctgtggctgaaaaagcaccgcttccgcctcgaccaggtgcagaacttctatccgtcgccgctggcgaactcaaccaccatgtattacaccgggaaaa       <  1:143400‑M1/147‑143 (MQ=255)                                                                                                                                  ttCATCGCGCGTACCGGGGTGCGCGGAGATGAAATAGGGTATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTCAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGTCCGGCTTCATCATCTTCGATAACGGCCcttctt      <  1:61072/149‑1 (MQ=255)                                                                                                                                    cATCtccgcgcaccccggtacgcgtgatgaagatatggtgaatctggcgctgtggctgaaaaagcaccgcttccgcctcgaccaggtgcagcacttctatccgtcgccgctggccaactcaaccaccatgtattacaccgggaaaaacc    >  1:376411‑M1/1‑4 (MQ=255)                                                                                                                                      tcaCGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCCTCTTCGATAACCGCCCTTCATCGGt  >  1:97190/1‑149 (MQ=255)                                                                                                                                       |                                                                                                                                                    CTTCGCCAGCGGGTTTTTCCCGGTGTAATACATGGTGGTTGAGTTCGCCAGCGGCGACGGATAGAAGTTCTGCACCTGGTCGAGGCGGAAGCGGTGCTTTTTCAGCCACAGCGCCAGATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGT  >  NC_000913_3_hsa_tpiA/3159287‑3159568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.