Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_hsa_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_hsa_tpiA 1299499–1300697 1300697 1–1199 50 [0] [0] 50 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 12994980 (0.000)48 (0.820) 42/274 0.5 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_hsa_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_hsa_tpiA/1299354‑1299646
                                                                                                                                                |                                                                                                                                                    
tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGACTAATTGAAATGATATTATTAATTCCACTGCCTTTGCTAGAGTAAAGTGCTAAataa                                                                                                                                                  >  2:978771‑M1/1‑145 (MQ=255)
    aTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                                                                                                                              <  2:673501‑M1/149‑9 (MQ=255)
         agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                         <  2:18061‑M1/149‑14 (MQ=255)
         agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                         >  2:824939‑M1/1‑136 (MQ=255)
           ttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCCTAATCCTGGGCTTGCTGAGGCATAATTGAAATGCTCTTATTTATTCATCTGCCGTGGGTAGAGGAAAGTGCTACataataataaattgt                                                                                                                                       >  1:721730‑M1/1‑134 (MQ=255)
            tagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                                      <  1:33568‑M1/149‑17 (MQ=255)
               ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                                                                                                                   >  1:736467‑M1/1‑130 (MQ=255)
                   aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattat                                                                                                                               >  1:839949‑M1/1‑126 (MQ=255)
                     ggATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATACTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCAACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattg                                                                                                                             >  2:1045791‑M1/1‑124 (MQ=255)
                       aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                           >  1:1038708‑M1/1‑122 (MQ=255)
                        tttCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgc                                                                                                                          <  1:537062‑M1/149‑29 (MQ=255)
                            cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatggttaaattattgtgcattt                                                                                                                      >  2:661082‑M1/1‑117 (MQ=255)
                             ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttt                                                                                                                     >  2:714777‑M1/1‑116 (MQ=255)
                                 cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCATGGGCTTGTTAAAGAATAATTCATATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                                 <  1:466023‑M1/149‑38 (MQ=255)
                                 cTGCTTCGCCTCACCATCATGATATTTTCGCCACATCATAATCCTGGTCTTGCTGAAGAATAATTGAAATGATAGTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                                 <  2:246347‑M1/149‑38 (MQ=255)
                                          ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCTTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttaaatactggaactg                                                                                                        >  2:321134‑M1/1‑103 (MQ=255)
                                          ctcACCATCATGTTACTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTGTGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactg                                                                                                        <  1:452188‑M1/149‑47 (MQ=255)
                                               catcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                                   >  2:790211‑M1/1‑98 (MQ=255)
                                                   atGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaa                                                                                               >  2:179245‑M1/1‑94 (MQ=255)
                                                    tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaa                                                                                              <  2:70993‑M1/149‑57 (MQ=255)
                                                    tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTCCTAAataataatcaatttttaaattactttgcatttcactactagacctctaaccagcaa                                                                                              >  2:471575‑M1/1‑93 (MQ=255)
                                                     gTTATTTTCGCCACATCATAATTCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                             <  2:627877‑M1/149‑58 (MQ=255)
                                                     gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATCTTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                             >  1:686299‑M1/1‑92 (MQ=255)
                                                     gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                             <  2:839949‑M1/149‑58 (MQ=255)
                                                     gTTATTTTCGCCACATAATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactacttgaactgtaatcagaaaa                                                                                             <  2:962619‑M1/149‑58 (MQ=255)
                                                      ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaag                                                                                            >  1:788710‑M1/1‑91 (MQ=255)
                                                               ccACATCATAATCCTGGGCTTGCTGAAGAATACTTGAAATGATATAATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatg                                                                                   >  1:829960‑M1/1‑82 (MQ=255)
                                                                aacatCATAATCCAGGGCTGTCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatga                                                                                  >  2:97876‑M1/2‑81 (MQ=255)
                                                                   atcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTTCTAAataatcatcaattgttaaattattgtgcatttcactacttgtaatgtaatgcgtaaggatgcacatgctca                                                                               >  2:956680‑M1/1‑78 (MQ=255)
                                                                    gcataATCCTGGGCGTGCTGAAGAATAATTGAAATGAGATTATTAATTCCACTGCTTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttag                                                                              <  2:212996‑M1/148‑73 (MQ=255)
                                                                          tCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGCGGAAATTGCTAAataataatcaattgttcaattattgtgcacttcactactggaactgtaataaggaaagatagaaatgcttagccaatc                                                                        >  1:697776‑M1/1‑71 (MQ=255)
                                                                              gggCTTGCTGAAGAATAATTGCAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaataagaaaacatagacatgcttagccaatctcta                                                                    >  2:50200‑M1/1‑67 (MQ=255)
                                                                                         agaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttccctatcggtactgtaatcagaaaagatagacatgtttagccaatctctatttgattgaat                                                         <  1:824939‑M1/149‑94 (MQ=255)
                                                                                              aatTGAAATGATATTATTAATTCCACTGCCTATGGTGTAGGATAATGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                                    <  1:978771‑M1/149‑99 (MQ=255)
                                                                                                  gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatg                                                >  2:693481‑M1/1‑47 (MQ=255)
                                                                                                          attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatccctctttgcttgaattgaaagatgtttgttaa                                        >  1:50778‑M1/1‑39 (MQ=255)
                                                                                                            tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                                      <  2:546064‑M1/149‑113 (MQ=255)
                                                                                                             attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggc                                     >  1:697260‑M1/1‑36 (MQ=255)
                                                                                                                      aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagctagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgca                            >  2:405658‑M1/1‑27 (MQ=255)
                                                                                                                      aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgca                            >  1:399699‑M1/1‑27 (MQ=255)
                                                                                                                       cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaa                           >  1:305347‑M1/1‑26 (MQ=255)
                                                                                                                         gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagc                         <  2:305347‑M1/149‑126 (MQ=255)
                                                                                                                              tGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatag                    <  1:179245‑M1/149‑131 (MQ=255)
                                                                                                                                  agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc                <  1:180711‑M1/149‑135 (MQ=255)
                                                                                                                                  agagGAAAGTGCTAAaatataatcaattgttaaattattgtgcactttactactggaactgtaatcaaaaaagatagacatgcttagctaatctctatttgattgaattgaaagatgtttgttaagccattgatgccagctatagattc                <  2:721730‑M1/149‑135 (MQ=255)
                                                                                                                                      gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactaccggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgat            >  1:160116‑M1/1‑11 (MQ=255)
                                                                                                                                         aGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaaaatgtttgttaaggcatggatgcaagcaataaattctgatacg         >  2:272870‑M1/1‑8 (MQ=255)
                                                                                                                                          gTGCTAAataataatcaattgttaaattattgtgcatttcactactggacctgtaatcagaaaagatagacatgcttagccaatctctatttgatttaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacgg        <  1:926822‑M1/149‑143 (MQ=255)
                                                                                                                                             cTAAataataatcaattgttaaattattttgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtca     >  2:783334‑M1/1‑4 (MQ=255)
                                                                                                                                                aataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaata  >  2:371045‑M1/1‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_hsa_tpiA/1299354‑1299646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.