Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_hsa_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_hsa_tpiA 1299499–1300697 1300697 1–1199 50 [0] [0] 50 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 12994980 (0.000)48 (0.820) 42/274 0.5 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_hsa_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATA  >  NC_000913_3_hsa_tpiA/1300553‑1300845
                                                                                                                                                 |                                                                                                                                                   
tcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagactaattgaaatgatattattaattccactgcctttgctagagtaaagtgctaaataa                                                                                                                                                  >  2:978771‑M2/146‑149 (MQ=255)
    attacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataat                                                                                                                                              <  2:673501‑M2/8‑1 (MQ=255)
         agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                         <  2:18061‑M2/13‑1 (MQ=255)
         agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                         >  2:824939‑M2/137‑149 (MQ=255)
           ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcctaatcctgggcttgctgaggcataattgaaatgctcttatttattcatctgccgtgggtagaggaaagtgctacATAATAATAAATTGt                                                                                                                                       >  1:721730‑M2/135‑149 (MQ=255)
            tagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                                      <  1:33568‑M2/16‑1 (MQ=255)
               ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                                   >  1:736467‑M2/131‑149 (MQ=255)
                   aaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattat                                                                                                                               >  1:839949‑M2/127‑149 (MQ=255)
                     ggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatactgggcttgctgaagaataattgaaatgatattattaattcaactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtg                                                                                                                             >  2:1045791‑M2/125‑149 (MQ=255)
                       atttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                           >  1:1038708‑M2/123‑149 (MQ=255)
                        tttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGc                                                                                                                          <  1:537062‑M2/28‑1 (MQ=255)
                            cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATGGTTAAATTATTGTGCAttt                                                                                                                      >  2:661082‑M2/118‑149 (MQ=255)
                             ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCatttt                                                                                                                     >  2:714777‑M2/117‑148 (MQ=255)
                                 ctgcttctcctcaccatcatgttattttcgccacatcataatcatgggcttgttaaagaataattcatatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                                 <  1:466023‑M2/37‑1 (MQ=255)
                                 ctgcttcgcctcaccatcatgatattttcgccacatcataatcctggtcttgctgaagaataattgaaatgatagtattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                                 <  2:246347‑M2/37‑1 (MQ=255)
                                          ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcttttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTAAATACTGGAACTg                                                                                                        >  2:321134‑M2/104‑149 (MQ=255)
                                          ctcaccatcatgttactttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgccttgtgtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTg                                                                                                        <  1:452188‑M2/46‑1 (MQ=255)
                                               catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                                   >  2:790211‑M2/99‑149 (MQ=255)
                                                   atgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                               >  2:179245‑M2/95‑149 (MQ=255)
                                                    tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                              <  2:70993‑M2/56‑1 (MQ=255)
                                                    tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtcctaaATAATAATCAATTTTTAAATTACTTTGCATTTCACTACTAGACCTCTAACCAGcaa                                                                                              >  2:471575‑M2/94‑149 (MQ=255)
                                                     gttattttcgccacatcataattctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                             <  2:627877‑M2/57‑1 (MQ=255)
                                                     gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatcttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                             >  1:686299‑M2/93‑149 (MQ=255)
                                                     gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                             <  2:839949‑M2/57‑1 (MQ=255)
                                                     gttattttcgccacataataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTTGAACTGTAATCAGaaaa                                                                                             <  2:962619‑M2/57‑1 (MQ=255)
                                                      ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAg                                                                                            >  1:788710‑M2/92‑149 (MQ=255)
                                                               ccacatcataatcctgggcttgctgaagaatacttgaaatgatataattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                   >  1:829960‑M2/83‑149 (MQ=255)
                                                                aacatcataatccagggctgtctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAtga                                                                                  >  2:97876‑M2/82‑148 (MQ=255)
                                                                   atcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagttctaaATAATCATCAATTGTTAAATTATTGTGCATTTCACTACTTGTAATGTAATGCGTAAGGATGCACATgctca                                                                               >  2:956680‑M2/79‑147 (MQ=255)
                                                                    gcataatcctgggcgtgctgaagaataattgaaatgagattattaattccactgcttttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAg                                                                              <  2:212996‑M2/72‑1 (MQ=255)
                                                                          tcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagcggaaattgctaaATAATAATCAATTGTTCAATTATTGTGCACTTCACTACTGGAACTGTAATAAGGAAAGATAGAAATGCTTAGCCAAtc                                                                        >  1:697776‑M2/72‑149 (MQ=255)
                                                                              gggcttgctgaagaataattgcaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATAAGAAAACATAGACATGCTTAGCCAATCTCTa                                                                    >  2:50200‑M2/68‑149 (MQ=255)
                                                                                         agaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCCCTATCGGTACTGTAATCAGAAAAGATAGACATGTTTAGCCAATCTCTATTTGattgaat                                                         <  1:824939‑M2/93‑1 (MQ=255)
                                                                                              aattgaaatgatattattaattccactgcctatggtgtaggataatgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                                    <  1:978771‑M2/98‑1 (MQ=255)
                                                                                                  gaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtg                                                >  2:693481‑M2/48‑149 (MQ=255)
                                                                                                          attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCCCTCTTTGCTTGAATTGAAAGATGTTTGTTaa                                        >  1:50778‑M2/40‑149 (MQ=255)
                                                                                                            tattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAgg                                      <  2:546064‑M2/112‑1 (MQ=255)
                                                                                                             attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGc                                     >  1:697260‑M2/37‑149 (MQ=255)
                                                                                                                      actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGCTAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCa                            >  2:405658‑M2/28‑149 (MQ=255)
                                                                                                                      actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCa                            >  1:399699‑M2/28‑149 (MQ=255)
                                                                                                                       ctgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                           >  1:305347‑M2/27‑149 (MQ=255)
                                                                                                                         gcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGc                         <  2:305347‑M2/125‑1 (MQ=255)
                                                                                                                              tggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAg                    <  1:179245‑M2/130‑1 (MQ=255)
                                                                                                                                  agaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc                <  1:180711‑M2/134‑1 (MQ=255)
                                                                                                                                  agaggaaagtgctaaAATATAATCAATTGTTAAATTATTGTGCACTTTACTACTGGAACTGTAATCAAAAAAGATAGACATGCTTAGCTAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGCCATTGATGCCAGCTATAGATTc                <  2:721730‑M2/134‑1 (MQ=255)
                                                                                                                                      gaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACCGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGAt            >  1:160116‑M2/12‑149 (MQ=255)
                                                                                                                                         agtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAAATGTTTGTTAAGGCATGGATGCAAGCAATAAATTCTGATACg         >  2:272870‑M2/9‑149 (MQ=255)
                                                                                                                                          gtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGACCTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTTAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACgg        <  1:926822‑M2/142‑1 (MQ=255)
                                                                                                                                             ctaaATAATAATCAATTGTTAAATTATTTTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCa     >  2:783334‑M2/5‑149 (MQ=255)
                                                                                                                                                aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATa  >  2:371045‑M2/2‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATA  >  NC_000913_3_hsa_tpiA/1300553‑1300845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.