Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1299499–1300697	1300697	1–1199	50 [0]	[0] 50	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1299498	0 (0.000)	48 (0.820)	42/274	0.5	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_hsa_tpiA	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_hsa_tpiA/1299354‑1299646                                                                                                                                                 |                                                                                                                                                     tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGACTAATTGAAATGATATTATTAATTCCACTGCCTTTGCTAGAGTAAAGTGCTAAataa                                                                                                                                                  >  2:978771‑M1/1‑145 (MQ=255)     aTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                                                                                                                              <  2:673501‑M1/149‑9 (MQ=255)          agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                         <  2:18061‑M1/149‑14 (MQ=255)          agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                         >  2:824939‑M1/1‑136 (MQ=255)            ttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCCTAATCCTGGGCTTGCTGAGGCATAATTGAAATGCTCTTATTTATTCATCTGCCGTGGGTAGAGGAAAGTGCTACataataataaattgt                                                                                                                                       >  1:721730‑M1/1‑134 (MQ=255)             tagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                                      <  1:33568‑M1/149‑17 (MQ=255)                ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                                                                                                                   >  1:736467‑M1/1‑130 (MQ=255)                    aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattat                                                                                                                               >  1:839949‑M1/1‑126 (MQ=255)                      ggATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATACTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCAACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattg                                                                                                                             >  2:1045791‑M1/1‑124 (MQ=255)                        aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                           >  1:1038708‑M1/1‑122 (MQ=255)                         tttCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgc                                                                                                                          <  1:537062‑M1/149‑29 (MQ=255)                             cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatggttaaattattgtgcattt                                                                                                                      >  2:661082‑M1/1‑117 (MQ=255)                              ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttt                                                                                                                     >  2:714777‑M1/1‑116 (MQ=255)                                  cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCATGGGCTTGTTAAAGAATAATTCATATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                                 <  1:466023‑M1/149‑38 (MQ=255)                                  cTGCTTCGCCTCACCATCATGATATTTTCGCCACATCATAATCCTGGTCTTGCTGAAGAATAATTGAAATGATAGTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                                 <  2:246347‑M1/149‑38 (MQ=255)                                           ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCTTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttaaatactggaactg                                                                                                        >  2:321134‑M1/1‑103 (MQ=255)                                           ctcACCATCATGTTACTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTGTGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactg                                                                                                        <  1:452188‑M1/149‑47 (MQ=255)                                                catcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                                   >  2:790211‑M1/1‑98 (MQ=255)                                                    atGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaa                                                                                               >  2:179245‑M1/1‑94 (MQ=255)                                                     tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaa                                                                                              <  2:70993‑M1/149‑57 (MQ=255)                                                     tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTCCTAAataataatcaatttttaaattactttgcatttcactactagacctctaaccagcaa                                                                                              >  2:471575‑M1/1‑93 (MQ=255)                                                      gTTATTTTCGCCACATCATAATTCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                             <  2:627877‑M1/149‑58 (MQ=255)                                                      gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATCTTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                             >  1:686299‑M1/1‑92 (MQ=255)                                                      gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                             <  2:839949‑M1/149‑58 (MQ=255)                                                      gTTATTTTCGCCACATAATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactacttgaactgtaatcagaaaa                                                                                             <  2:962619‑M1/149‑58 (MQ=255)                                                       ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaag                                                                                            >  1:788710‑M1/1‑91 (MQ=255)                                                                ccACATCATAATCCTGGGCTTGCTGAAGAATACTTGAAATGATATAATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatg                                                                                   >  1:829960‑M1/1‑82 (MQ=255)                                                                 aacatCATAATCCAGGGCTGTCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatga                                                                                  >  2:97876‑M1/2‑81 (MQ=255)                                                                    atcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTTCTAAataatcatcaattgttaaattattgtgcatttcactacttgtaatgtaatgcgtaaggatgcacatgctca                                                                               >  2:956680‑M1/1‑78 (MQ=255)                                                                     gcataATCCTGGGCGTGCTGAAGAATAATTGAAATGAGATTATTAATTCCACTGCTTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttag                                                                              <  2:212996‑M1/148‑73 (MQ=255)                                                                           tCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGCGGAAATTGCTAAataataatcaattgttcaattattgtgcacttcactactggaactgtaataaggaaagatagaaatgcttagccaatc                                                                        >  1:697776‑M1/1‑71 (MQ=255)                                                                               gggCTTGCTGAAGAATAATTGCAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaataagaaaacatagacatgcttagccaatctcta                                                                    >  2:50200‑M1/1‑67 (MQ=255)                                                                                          agaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttccctatcggtactgtaatcagaaaagatagacatgtttagccaatctctatttgattgaat                                                         <  1:824939‑M1/149‑94 (MQ=255)                                                                                               aatTGAAATGATATTATTAATTCCACTGCCTATGGTGTAGGATAATGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                                    <  1:978771‑M1/149‑99 (MQ=255)                                                                                                   gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatg                                                >  2:693481‑M1/1‑47 (MQ=255)                                                                                                           attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatccctctttgcttgaattgaaagatgtttgttaa                                        >  1:50778‑M1/1‑39 (MQ=255)                                                                                                             tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                                      <  2:546064‑M1/149‑113 (MQ=255)                                                                                                              attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggc                                     >  1:697260‑M1/1‑36 (MQ=255)                                                                                                                       aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagctagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgca                            >  2:405658‑M1/1‑27 (MQ=255)                                                                                                                       aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgca                            >  1:399699‑M1/1‑27 (MQ=255)                                                                                                                        cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaa                           >  1:305347‑M1/1‑26 (MQ=255)                                                                                                                          gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagc                         <  2:305347‑M1/149‑126 (MQ=255)                                                                                                                               tGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatag                    <  1:179245‑M1/149‑131 (MQ=255)                                                                                                                                   agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc                <  1:180711‑M1/149‑135 (MQ=255)                                                                                                                                   agagGAAAGTGCTAAaatataatcaattgttaaattattgtgcactttactactggaactgtaatcaaaaaagatagacatgcttagctaatctctatttgattgaattgaaagatgtttgttaagccattgatgccagctatagattc                <  2:721730‑M1/149‑135 (MQ=255)                                                                                                                                       gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactaccggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgat            >  1:160116‑M1/1‑11 (MQ=255)                                                                                                                                          aGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaaaatgtttgttaaggcatggatgcaagcaataaattctgatacg         >  2:272870‑M1/1‑8 (MQ=255)                                                                                                                                           gTGCTAAataataatcaattgttaaattattgtgcatttcactactggacctgtaatcagaaaagatagacatgcttagccaatctctatttgatttaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacgg        <  1:926822‑M1/149‑143 (MQ=255)                                                                                                                                              cTAAataataatcaattgttaaattattttgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtca     >  2:783334‑M1/1‑4 (MQ=255)                                                                                                                                                 aataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaata  >  2:371045‑M1/1‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     TCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_hsa_tpiA/1299354‑1299646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑