Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,299,499	Δ1,199 bp	100%	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1299499–1300695	1300697	3–1199	39 [0]	[0] 39	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1299498	0 (0.000)	35 (1.120)	31/268	NT	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_hsa_tpiA	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTG  >  NC_000913_3_hsa_tpiA/1299351‑1299641                                                                                                                                                    |                                                                                                                                                ttcgCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                                                                                                                <  1:262418‑M1/149‑2 (MQ=255)   ctcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAata                                                                                                                                              >  1:343889‑M1/1‑146 (MQ=255)        aTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                                                                                                                         <  1:317514‑M1/149‑9 (MQ=255)            cAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATGATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                                                                                                                     >  2:547760‑M1/1‑137 (MQ=255)                   ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                                                                                                              >  1:547672‑M1/1‑130 (MQ=255)                     ataAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataataaattgttaaatt                                                                                                                            >  1:532201‑M1/1‑128 (MQ=255)                      taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatta                                                                                                                           >  1:354870‑M1/1‑127 (MQ=255)                                cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATCATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAAGAAAGTGCTAAataataatcacttgttaaattattgtgcattt                                                                                                                 >  2:127930‑M1/1‑117 (MQ=255)                                 ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATACTCCTGGACTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGACTTTGGTAGAGGAAAGTGCTAAataatactcaattattaaattattgtacatttc                                                                                                                >  2:249353‑M1/1‑116 (MQ=255)                                     cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                            >  1:33081‑M1/1‑112 (MQ=255)                                              ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAAAATACTTGGAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcacttgttaaattattgtgcattttcatactcgaactg                                                                                                   >  1:365847‑M1/1‑103 (MQ=255)                                                   catcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtactc                                                                                              >  2:238649‑M1/1‑98 (MQ=255)                                                    atcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                             >  2:265482‑M1/1‑97 (MQ=255)                                                       atGTTATTTTCGCCAGATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaa                                                                                          <  2:233021‑M1/149‑56 (MQ=255)                                                             ttttCGCCAAATCATAATTCTGAGCTTTCTGAAGAATAATTTAAATGATAATATTAATTCCACTGCCTTTTGTAGATGAAAGTGCTAAataataatcaattgctaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                    <  2:316747‑M1/149‑62 (MQ=255)                                                              tttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatag                                                                                   <  1:114774‑M1/149‑63 (MQ=255)                                                                        tcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttag                                                                         >  1:499446‑M1/1‑77 (MQ=255)                                                                         tataATCCTGGATTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                        <  2:605392‑M1/148‑74 (MQ=255)                                                                         catAATCCTGGGCTTGCTGAAGAATAATTGAAATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                        <  1:544643‑M1/149‑74 (MQ=255)                                                                              tCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatgagcaaagatagaaatgcttagccaatc                                                                   >  1:448088‑M1/1‑71 (MQ=255)                                                                                          tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaatttatagtgcatttcactactggaactgtattcagaaaaaatagacatgcttagccaatctctattagattg                                                       >  2:374404‑M1/1‑59 (MQ=255)                                                                                          tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgaaagtgtaatccgaaaagataaccatgcttacccaagctctctttgattg                                                       >  2:19568‑M1/1‑59 (MQ=255)                                                                                                    ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctttttgattgaattgaaaga                                             >  1:220218‑M1/1‑49 (MQ=255)                                                                                                    ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaaga                                             >  1:317667‑M1/1‑49 (MQ=255)                                                                                                    ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaaaatctatttgattgaattgacaga                                             >  2:550558‑M1/1‑49 (MQ=255)                                                                                                     ttaaatgATATTATTAAGTCCACTGCCTTTGGTAGAGGAAAGTGCTAActaataaatgattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagat                                            <  1:294455‑M1/147‑102 (MQ=255)                                                                                                       aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgt                                          >  1:305242‑M1/1‑46 (MQ=255)                                                                                                        aaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtt                                         >  1:314340‑M1/1‑45 (MQ=255)                                                                                                         aTGATAATATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatcgacatgcttagccaatctctatttgattgaattgaaagatgttt                                        <  1:239514‑M1/149‑106 (MQ=255)                                                                                                            atatTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagacttttgtt                                     >  1:268338‑M1/1‑41 (MQ=255)                                                                                                               ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgattacccaatctctatttgattgaattgaaagctgtttattaag                                  >  2:549741‑M1/1‑38 (MQ=255)                                                                                                                tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                                 >  1:162297‑M1/1‑37 (MQ=255)                                                                                                                  ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggca                               >  1:394036‑M1/1‑35 (MQ=255)                                                                                                                     aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattagggtgcatttcactactggaactgtaatcagaaaagatagacaagcttagcaaatctctatttgcttaaaatgaaagaggtctctgaaggcatgg                            >  2:202802‑M1/1‑32 (MQ=255)                                                                                                                            tGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaag                     <  1:49187‑M1/149‑125 (MQ=255)                                                                                                                                     tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagatt            <  1:486564‑M1/149‑134 (MQ=255)                                                                                                                                         ggAAAGTTCTAAataataatcaattggtaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctga        <  1:59260‑M1/149‑138 (MQ=255)                                                                                                                                              gTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacgg   <  1:89463‑M1/149‑143 (MQ=255)                                                                                                                                               tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagatttggatacggt  >  2:468039‑M1/1‑6 (MQ=255)                                                                                                                                                    |                                                                                                                                                TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTG  >  NC_000913_3_hsa_tpiA/1299351‑1299641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑