Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,299,499	Δ1,199 bp	100%	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1299499–1300695	1300697	3–1199	39 [0]	[0] 39	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1299498	0 (0.000)	35 (1.120)	31/268	NT	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_hsa_tpiA	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913_3_hsa_tpiA/1300550‑1300840                                                                                                                                                     |                                                                                                                                               ttcgcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                <  1:262418‑M2/1‑1 (MQ=255)   ctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaata                                                                                                                                              >  1:343889‑M2/147‑149 (MQ=255)        attacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataat                                                                                                                                         <  1:317514‑M2/8‑1 (MQ=255)            cagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaatgattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAt                                                                                                                                     >  2:547760‑M2/138‑149 (MQ=255)                   ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                              >  1:547672‑M2/131‑149 (MQ=255)                     ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATAAATTGTTAAatt                                                                                                                            >  1:532201‑M2/129‑149 (MQ=255)                      taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatta                                                                                                                           >  1:354870‑M2/128‑149 (MQ=255)                                cttaactgcttctcctcaccatcatgttattttcgccacatcatcatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaagaaagtgctaaATAATAATCACTTGTTAAATTATTGTGCAttt                                                                                                                 >  2:127930‑M2/118‑149 (MQ=255)                                 ttaactgcttctcctcaccatcatgttattttcgccacatcatactcctggacttgctgaagaataattgaaatgatattattaattccactgactttggtagaggaaagtgctaaATAATACTCAATTATTAAATTATTGTACATTTc                                                                                                                >  2:249353‑M2/117‑149 (MQ=255)                                     ctgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                            >  1:33081‑M2/113‑149 (MQ=255)                                              ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaaaaatacttggaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCACTTGTTAAATTATTGTGCATTTTCATACTCGAACTg                                                                                                   >  1:365847‑M2/104‑149 (MQ=255)                                                   catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTACTc                                                                                              >  2:238649‑M2/99‑149 (MQ=255)                                                    atcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                             >  2:265482‑M2/98‑149 (MQ=255)                                                       atgttattttcgccagatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                          <  2:233021‑M2/55‑1 (MQ=255)                                                             ttttcgccaaatcataattctgagctttctgaagaataatttaaatgataatattaattccactgccttttgtagatgaaagtgctaaATAATAATCAATTGCTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                    <  2:316747‑M2/61‑1 (MQ=255)                                                              tttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAg                                                                                   <  1:114774‑M2/62‑1 (MQ=255)                                                                        tcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAg                                                                         >  1:499446‑M2/78‑149 (MQ=255)                                                                         tataatcctggatttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                        <  2:605392‑M2/73‑1 (MQ=255)                                                                         cataatcctgggcttgctgaagaataattgaaattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                        <  1:544643‑M2/73‑1 (MQ=255)                                                                              tcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATGAGCAAAGATAGAAATGCTTAGCCAAtc                                                                   >  1:448088‑M2/72‑149 (MQ=255)                                                                                          tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAATTTATAGTGCATTTCACTACTGGAACTGTATTCAGAAAAAATAGACATGCTTAGCCAATCTCTATtagattg                                                       >  2:374404‑M2/60‑149 (MQ=255)                                                                                          tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGAAAGTGTAATCCGAAAAGATAACCATGCTTACCCAAGCTCTCTttgattg                                                       >  2:19568‑M2/60‑149 (MQ=255)                                                                                                    ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTTTTTGATTGAATTGAAAGa                                             >  1:220218‑M2/50‑149 (MQ=255)                                                                                                    ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                             >  1:317667‑M2/50‑149 (MQ=255)                                                                                                    ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAAATCTATTTGATTGAATTGACAGa                                             >  2:550558‑M2/50‑149 (MQ=255)                                                                                                     ttaaatgatattattaagtccactgcctttggtagaggaaagtgctaaCTAATAAATGATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAt                                            <  1:294455‑M2/101‑1 (MQ=255)                                                                                                       aaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgt                                          >  1:305242‑M2/47‑149 (MQ=255)                                                                                                        aatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                         >  1:314340‑M2/46‑149 (MQ=255)                                                                                                         atgataatattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATCGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                        <  1:239514‑M2/105‑1 (MQ=255)                                                                                                            atattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGActtttgtt                                     >  1:268338‑M2/42‑149 (MQ=255)                                                                                                               ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGATTACCCAATCTCTATTTGATTGAATTGAAAGCTGTTTATTAAg                                  >  2:549741‑M2/39‑149 (MQ=255)                                                                                                                tattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAgg                                 >  1:162297‑M2/38‑149 (MQ=255)                                                                                                                  ttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCa                               >  1:394036‑M2/36‑149 (MQ=255)                                                                                                                     attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTAGGGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAAGCTTAGCAAATCTCTATTTGCTTAAAATGAAAGAGGTCTCTGAAGGCATgg                            >  2:202802‑M2/33‑149 (MQ=255)                                                                                                                            tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                     <  1:49187‑M2/124‑1 (MQ=255)                                                                                                                                     tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAtt            <  1:486564‑M2/133‑1 (MQ=255)                                                                                                                                         ggaaagttctaaATAATAATCAATTGGTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa        <  1:59260‑M2/137‑1 (MQ=255)                                                                                                                                              gtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACgg   <  1:89463‑M2/142‑1 (MQ=255)                                                                                                                                               tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTTGGATACGGt  >  2:468039‑M2/7‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                               ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913_3_hsa_tpiA/1300550‑1300840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑