New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1498531	23 (0.630)	3 (0.090)	3/260	NT	12.1%	intergenic (+20/‑120)	opgD/ydcH	OPG biosynthetic periplasmic protein/DUF465 family protein
	?	NC_000913_3_hsa_tpiA	= 1498539	22 (0.640)					intergenic (+28/‑112)	opgD/ydcH	OPG biosynthetic periplasmic protein/DUF465 family protein

ACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTT  >  NC_000913_3_hsa_tpiA/1498383‑1498673                                                                                                                                                     |                                                                                                                                               aCTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCT                                                                                                                                                >  2:655716‑M1/1‑149 (MQ=255)        cGGTGGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCgg                                                                                                                                         <  1:142791/149‑1 (MQ=255)                atGCGGATGTATCTACGTTGTCAGGGGGACGGTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGaggtgcc                                                                                                                                 >  1:232335‑M2/1‑142 (MQ=255)                 tGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGgggtgccg                                                                                                                                <  1:170825‑M2/149‑9 (MQ=255)                   gggaTGTATCTACGTTGTCGGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGGGCCGGATAAACGTGAGTATGTGGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTcccgaagaaggtgccgaa                                                                                                                              <  2:633527‑M1/148‑19 (MQ=255)                          aTCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTTTTCGGGGTGTGCCGTCTGGTTaa                                                                                                                       >  2:234944/1‑149 (MQ=255)                           tcgaCGTTGTCAGGGGGACGCAGCTAGTATATCTTGGCTGTATCAGTTTGTCCTGCCATCGCCTGATAAACGTCAGTTTGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTaaa                                                                                                                      <  2:281880/146‑1 (MQ=255)                           tCTACGCTGTCAGCGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGATCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTaaa                                                                                                                      <  1:147395/149‑1 (MQ=255)                             tACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGGTAATCGTTTTTTCTTCGGCACCTTCTTCGGCAGGTGCCGTCTAGTTAAaca                                                                                                                    >  2:376515/1‑149 (MQ=255)                                gtggggggggggACGCGATGAGTGAACCGGGGGGGTATCAGTAGTTGCCGCAAGCGCCGTATTGAGGTATGTAGGGTGGCGATCGCGTGATGAGTTGATCGTTTTTTCGTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGa                                                                                                                 <  2:75610/142‑1 (MQ=255)                                gTTGTCGGGGGGACGCTATCAGTGAAGCAGGGCGGTAGCAGTATTTCGCGCCCGCGCCGGATAAACGTCAGTATATTGACGACCGCGTTATGTGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTACACACGa                                                                                                                 <  2:76948/149‑1 (MQ=255)                                 ttGTTAGGGGGACGCTATCAGTGCAACATGGCTGTATCAGTATTTACCGCCAGCGCCGGATAAACGTCAGTATGTTGAAGACCGCGTCATGAGTCAATCGTTTTATCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGAt                                                                                                                <  1:581777/149‑1 (MQ=255)                                  tGTCAGGGGGACGGTATCAGTGAAACATGGCTGTATTAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGCCCGCGTGATGAGTTAATCGTTTTTTCTTCGGTACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATc                                                                                                               <  2:187217/149‑1 (MQ=255)                                      aGGGGGACGCTATTAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAATATGTTGACGACCGCGGGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACCATcccac                                                                                                           >  2:214721/1‑147 (MQ=255)                                       ggggggCGCTATCAGGTGAATTTGGTTGGAGCAGTATTGCACCGCAGTGCCGGATGAACGTCAGTATGTTGACGACCGCGTGCTGAGTTAATGGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCt                                                                                                          <  2:74065/149‑1 (MQ=255)                                                                     tCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATc                                                                            >  1:512412/1‑149 (MQ=255)                                                                              cgcggcAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCCTTTGTTCGTCGGCAACTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGgtgt                                                                   <  2:518713/144‑1 (MQ=255)                                                                                   cAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCAGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTAtt                                                              >  1:494828/1‑149 (MQ=255)                                                                                     gcgcCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGGCTGGGTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTc                                                            <  1:121585/149‑1 (MQ=255)                                                                                      cgcCGGATAAACGTCAGTATGGTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCa                                                           <  1:658204/149‑1 (MQ=255)                                                                                       gcCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAg                                                          >  1:547757/1‑149 (MQ=255)                                                                                                  gTCAGGATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGt                                               <  2:499197/149‑1 (MQ=255)                                                                                                       tATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTACATGAGTCATCTGCTGGTGTGTCTTTCAGTTACCCCTTGTCCGcc                                          >  1:195329/1‑149 (MQ=255)                                                                                                           ttGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTa                                      >  1:416960/1‑149 (MQ=255)                                                                                                                gacCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTcccgaagaaggt                                                                                                                                    <  2:173579‑M1/50‑13 (MQ=255)                                                                                                                gacCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTcccgaagaaggt                                                                                                                                    >  1:173579‑M1/1‑38 (MQ=255)                                                                                                                          tgaGTTAATCGTTTCTTATGAGGCTCCTTCTTCTGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTAt                       <  1:13729/149‑1 (MQ=255)                                                                                                                                 atggTTTTTTGTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCa                <  1:383558/146‑1 (MQ=255)                                                                                                                                     attattcatcGGATCCATCGTCGTGTGGTGCCGGCTGGTTAAACACGATCACGCTCGCATTTTTCCCTAATTCAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTAGGTTTCCAGAAt            <  2:414602/145‑1 (MQ=255)                                                                                                                                            ttcGGCACCTTCTTCGGGaggtgccgaagaaaaaacgattaactcatcacgcggtcgtccacatactcacgtttatccggccctggcgggaaatactgatacagccatgtttcactgatagcgtccccccgacaacgtagatacatccc     <  2:633527‑M2/149‑132 (MQ=255)                                                                                                                                              cGGCACCCcccgaagaaggtgccgaagaaaaaacgattaactcatcacgcggtcgtcaacatactgacgtttatccggcgctggcgggaaatactgatacagccatgtttcactgatagcgtccccctgacaacgtagatacatccgca   <  1:170825‑M1/149‑142 (MQ=255)                                                                                                                                               ggCACCTcccgaagaaggtgccgaagaaaaaacgattaactcatcacgcggtcgtcaacatactgacgtttatccggcgctggcgggaaatactgatacagccatgtttcactgataccgtccccctgacaacgtagatacatccgcat  >  1:232335‑M1/1‑7 (MQ=255)                                                                                                                                                  aCCTTCTTCGGGaggtgccgaagaaaaaacgattaactcatcacgcggtc                                                                                                  <  2:173579‑M2/50‑39 (MQ=255)                                                                                                                                                  aCCTTCTTCGGGaggtgccgaagaaaaaacgattaactcatcacgcggtc                                                                                                  >  1:173579‑M2/1‑12 (MQ=255)                                                                                                                                                     |                                                                                                                                               ACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTT  >  NC_000913_3_hsa_tpiA/1498383‑1498673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.