Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1978503–1979268	1979278	11–776	30 [0]	[0] 29	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1978502	0 (0.000)	25 (0.740)	22/260	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_hsa_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

AAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCT  >  NC_000913_3_hsa_tpiA/1978357‑1978645                                                                                                                                                  |                                                                                                                                                aaaTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGAGGAAAAGACCTCTGATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                                                              >  1:327986‑M1/1‑146 (MQ=255)  aaTGGGTCTTTACACTTATCTAAAATTTTTCCTAAATCGACGCAACTGTCCTCATCACTACACACACATACAACGGAGCGGGGCCGACATTTAAACTACTACGTTATGCAGATCACACAAATAACCAACTTACTTTACATAACTgcaac                                                                                                                                             >  2:541749/1‑145 (MQ=255)          tttACACTTATCTAAGATTTTTCCTAAATCGACGTAACTGTACTCGTAACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGAGCACACAAAACAATCAATTACTTAACATAAATGtataagtcataa                                                                                                                                     >  2:544181‑M1/1‑137 (MQ=255)                     cgaagaTTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                          <  2:484409‑M1/147‑24 (MQ=255)                                 cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                              >  1:617292‑M1/1‑114 (MQ=255)                                  tAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatc                                                                                                             >  1:634480‑M1/1‑113 (MQ=255)                                                tGTACTCGTCACTACACGCACATACAAAGGAGGGGGGCTGCGATTGTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAAATAAATGtataagtcaaactcttgttttgggtgtatttcaatctgtaaaaaagcttt                                                                                               >  2:394626‑M1/1‑99 (MQ=255)                                                              cacGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagca                                                                                 <  1:660888‑M1/149‑65 (MQ=255)                                                                cGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaag                                                                               <  1:566712‑M1/149‑67 (MQ=255)                                                                   acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaa                                                                            <  2:513742‑M1/149‑70 (MQ=255)                                                                   acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaa                                                                            >  1:316721‑M1/1‑80 (MQ=255)                                                                   acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaa                                                                            >  1:316854‑M1/1‑80 (MQ=255)                                                                     aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaa                                                                          >  1:233593‑M1/1‑78 (MQ=255)                                                                      tACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgcttcgctagcaagcaaaaa                                                                         <  1:601179‑M1/149‑73 (MQ=255)                                                                      tACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                                                                         <  1:610068‑M1/149‑73 (MQ=255)                                                                         aaCGGAGGTGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatga                                                                      <  1:106971‑M1/149‑76 (MQ=255)                                                                                                aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggat                                               >  1:410692‑M1/1‑51 (MQ=255)                                                                                                    atGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGgataggtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaatcatcgaaatgggatgttg                                           >  1:29227‑M1/1‑47 (MQ=255)                                                                                                        gTGATGCAGATCACATAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgca                                       <  1:481572‑M1/149‑107 (MQ=255)                                                                                                        gTGATGCACATCACACAAAACTCTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgca                                       <  2:46992‑M1/149‑107 (MQ=255)                                                                                                           aTGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctggtaaaaagtttttcgctatgctagcaagcaaaaatgaaacaggaatgatcgaaatgggatgttgcgcacag                                    >  1:634272‑M1/1‑40 (MQ=255)                                                                                                                   cacacaAAACACTCAATTACTTAACATAAATGtataagtcatactttttttttttttttatttcaatctgttaaaaagtttttcgctaagttagcaagtaaaaatgaaataggaatattcgaaatgggatgttgcacacataaaaaata                            >  2:379320‑M1/1‑32 (MQ=255)                                                                                                                      acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggttttatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataact                         >  1:364501‑M1/1‑29 (MQ=255)                                                                                                                        aaaaCACTGCATTACATAACACAAATGgataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgatacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactca                       <  1:177498‑M1/149‑123 (MQ=255)                                                                                                                        aaaaCACTCAATTACTTAACATAAAGGtataagtcatacttttgttttgggtgtatttcaatctgttaacaagtttttcgctacgctagcaagcaaaaatgcaacaggagtaatcgaaatgggatgttgcacacagtcaaaataactca                       >  1:521357‑M1/1‑27 (MQ=255)                                                                                                                               tCAATTAATTAACATAAATGtataatttctacttttgttttggttctattttaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaa                <  1:214565‑M1/149‑130 (MQ=255)                                                                                                                                  aTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataa             <  1:212377‑M1/149‑133 (MQ=255)                                                                                                                                       ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatc        >  1:93999‑M1/1‑12 (MQ=255)                                                                                                                                            aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggaggttgcgcacagtcaaaataactcaccgtaaataatcatctgcta   >  1:568839‑M1/1‑7 (MQ=255)                                                                                                                                             tAAATGtataagtcatacttttgtattgggtgtatttccatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctat  >  2:176649‑M1/1‑6 (MQ=255)                                                                                                                                                  |                                                                                                                                                AAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCT  >  NC_000913_3_hsa_tpiA/1978357‑1978645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑