Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1978503–1979268	1979278	11–776	30 [0]	[0] 29	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1978502	0 (0.000)	25 (0.740)	22/260	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_hsa_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

TGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTAT  >  NC_000913_3_hsa_tpiA/1979133‑1979421                                                                                                                                                   |                                                                                                                                               aaatgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggagggaggaaaagacctctgataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgtat                                                                                                                                              >  1:327986‑M2/147‑149 (MQ=255)          tttacacttatctaagatttttcctaaatcgacgtaactgtactcgtaactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagagcacacaaaacaatcaattacttaacataaatgTATAAGTCAtaa                                                                                                                                     >  2:544181‑M2/138‑148 (MQ=255)                     cgaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                          <  2:484409‑M2/23‑1 (MQ=255)                                 ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                              >  1:617292‑M2/115‑149 (MQ=255)                                  taaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATc                                                                                                             >  1:634480‑M2/114‑149 (MQ=255)                                                tgtactcgtcactacacgcacatacaaaggaggggggctgcgattgtcaataatgcgtgatgcagatcacacaaaacactcaattacttaaaataaatgTATAAGTCAAACTCTTGTTTTGGGTGTATTTCAATCTGTAAAAAAGcttt                                                                                               >  2:394626‑M2/100‑149 (MQ=255)                                                              cacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagca                                                                                 <  1:660888‑M2/64‑1 (MQ=255)                                                                cgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaag                                                                               <  1:566712‑M2/66‑1 (MQ=255)                                                                   acatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAgcaagcaa                                                                            >  1:316721‑M2/81‑149 (MQ=255)                                                                   acatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAgcaagcaa                                                                            >  1:316854‑M2/81‑149 (MQ=255)                                                                   acatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAgcaagcaa                                                                            <  2:513742‑M2/69‑1 (MQ=255)                                                                     atacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaa                                                                          >  1:233593‑M2/79‑149 (MQ=255)                                                                      tacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTTCGCTAGCAAGCaaaaa                                                                         <  1:601179‑M2/72‑1 (MQ=255)                                                                      tacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaaa                                                                         <  1:610068‑M2/72‑1 (MQ=255)                                                                         aacggaggtgggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGa                                                                      <  1:106971‑M2/75‑1 (MQ=255)                                                                                                aataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGAt                                               >  1:410692‑M2/52‑149 (MQ=255)                                                                                                    atgcgtgatgcagatcacacaaaacactcaattacttaacataaatgGATAGGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATCATCGAAATGGGATGTTg                                           >  1:29227‑M2/48‑149 (MQ=255)                                                                                                        gtgatgcagatcacataaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGca                                       <  1:481572‑M2/106‑1 (MQ=255)                                                                                                        gtgatgcacatcacacaaaactctcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGca                                       <  2:46992‑M2/106‑1 (MQ=255)                                                                                                           atgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGGTAAAAAGTTTTTCGCTATGCTAGCAAGCAAAAATGAAACAGGAATGATCGAAATGGGATGTTGCGCACAg                                    >  1:634272‑M2/41‑149 (MQ=255)                                                                                                                   cacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTTTTTTTTTTTTATTTCAATCTGTTAAAAAGTTTTTCGCTAAGTTAGCAAGTAAAAATGAAATAGGAATATTCGAAATGGGATGTTGCACACATAAAAAATa                            >  2:379320‑M2/33‑149 (MQ=255)                                                                                                                      acaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGTTTTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACt                         >  1:364501‑M2/30‑149 (MQ=255)                                                                                                                        aaaacactgcattacataacacaaatgGATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGATACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCa                       <  1:177498‑M2/122‑1 (MQ=255)                                                                                                                        aaaacactcaattacttaacataaaggTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAACAAGTTTTTCGCTACGCTAGCAAGCAAAAATGCAACAGGAGTAATCGAAATGGGATGTTGCACACAGTCAAAATAACTCa                       >  1:521357‑M2/28‑149 (MQ=255)                                                                                                                               tcaattaattaacataaatgTATAATTTCTACTTTTGTTTTGGTTCTATTTTAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTaaa                <  1:214565‑M2/129‑1 (MQ=255)                                                                                                                                  attacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAaataa             <  1:212377‑M2/132‑1 (MQ=255)                                                                                                                                       ttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatcatc        >  1:93999‑M2/13‑149 (MQ=255)                                                                                                                                            ataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGAGGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCta   >  1:568839‑M2/8‑149 (MQ=255)                                                                                                                                             taaatgTATAAGTCATACTTTTGTATTGGGTGTATTTCCATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCtat  >  2:176649‑M2/7‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                               TGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTAT  >  NC_000913_3_hsa_tpiA/1979133‑1979421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑