Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1299499–1300697 1300697 1–1199 62 [0] [0] 62 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)60 (1.270) 49/282 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  NC_000913_3_pae_tpiA/1300550‑1300843
                                                                                                                                                    |                                                                                                                                                 
ttctcaaattacagttagttataaggatttccttaactgattctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataatttaaatgatattattaattcaactgcctttggtagcggaaagtgctaaa                                                                                                                                                   >  2:25047‑M2/149‑149 (MQ=255)
   tcaaaatacagttagttataaggatttccttaactgctactcctaaccatcaaattatattcgccacatcataatcctgggcatgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataa                                                                                                                                                <  2:32670‑M2/4‑1 (MQ=255)
      aattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                             <  2:699265‑M2/7‑1 (MQ=255)
            agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                       <  1:710750‑M2/13‑1 (MQ=255)
                  ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                                 <  2:482227‑M2/19‑1 (MQ=255)
                    ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgatggagaataagttaaatgatattattaattccactgcctttggtagtgaaaagtgctaaATAATAATCAATTGTTAAatt                                                                                                                               >  2:519237‑M2/129‑149 (MQ=255)
                         gatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgt                                                                                                                          >  1:599396‑M2/124‑149 (MQ=255)
                           tttccttaactgcttctcctcaccatcaagttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccagtgcctttggtagaggaaagtgctaaATAATACTAAATTCttaacatattattc                                                                                                                        >  2:345681‑M2/122‑145 (MQ=255)
                             tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATAAATTGTTAAATTATTGTGCAt                                                                                                                      >  2:333628‑M2/120‑149 (MQ=255)
                               cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaaagatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                    <  1:117615‑M2/32‑1 (MQ=255)
                                   actgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCact                                                                                                                >  1:569309‑M2/114‑149 (MQ=255)
                                      gcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                             <  1:60352‑M2/39‑1 (MQ=255)
                                                      ctgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                             <  2:821035‑M2/55‑1 (MQ=255)
                                                      atgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                             >  2:388306‑M2/95‑149 (MQ=255)
                                                           attttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                        <  1:86152‑M2/60‑1 (MQ=255)
                                                                  ccccacaataatcctgtgctcgctaaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                 <  2:164412‑M2/67‑1 (MQ=255)
                                                                  ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                 >  1:217280‑M2/83‑149 (MQ=255)
                                                                  ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                 >  1:595092‑M2/83‑149 (MQ=255)
                                                                  ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTAATCTGCTGTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                 <  1:416172‑M2/67‑1 (MQ=255)
                                                                   cagagcataatcctgggcttgctgaagaataattgaaatgatattatgaatgccactgactttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCGGTACTGGTACTGTGATCAGAAAAGATAGACATGc                                                                                <  2:191904‑M2/68‑1 (MQ=255)
                                                                     catcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCtt                                                                              >  1:5077‑M2/80‑149 (MQ=255)
                                                                       taataagcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATGGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAg                                                                            <  2:393774‑M2/72‑1 (MQ=255)
                                                                         ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTTTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAACGATAGAAATGCTTAGcc                                                                          >  1:402747‑M2/76‑149 (MQ=255)
                                                                         ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGcc                                                                          >  1:650435‑M2/76‑149 (MQ=255)
                                                                             tcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtc                                                                      >  1:788472‑M2/72‑149 (MQ=255)
                                                                                    cttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAttt                                                               <  2:650435‑M2/85‑1 (MQ=255)
                                                                                        ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           <  1:653028‑M2/89‑1 (MQ=255)
                                                                                        ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           >  2:351272‑M2/61‑149 (MQ=255)
                                                                                        ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           <  1:384779‑M2/89‑1 (MQ=255)
                                                                                          gaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgattga                                                         >  1:296666‑M2/59‑149 (MQ=255)
                                                                                           aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                    <  2:208278‑M2/32‑1 (MQ=255)
                                                                                           aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                    >  1:208278‑M2/58‑89 (MQ=255)
                                                                                             gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATACTCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGCAAAGATAGACATGCTTCTCCACTCTCTATTTAattcaatt                                                      >  2:457778‑M2/56‑149 (MQ=255)
                                                                                             gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaatt                                                      <  2:217280‑M2/94‑1 (MQ=255)
                                                                                             gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaatt                                                      <  1:211948‑M2/94‑1 (MQ=255)
                                                                                              aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtc                                                                      >  1:442223‑M2/55‑132 (MQ=255)
                                                                                              aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtc                                                                      <  2:442223‑M2/78‑1 (MQ=255)
                                                                                              aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattg                                                     >  2:427334‑M2/55‑149 (MQ=255)
                                                                                                taattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           >  2:658432‑M2/53‑141 (MQ=255)
                                                                                                taattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           <  1:658432‑M2/89‑1 (MQ=255)
                                                                                                 aattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                                  <  2:361309‑M2/98‑1 (MQ=255)
                                                                                                   ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                                >  2:820824‑M2/50‑149 (MQ=255)
                                                                                                   ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                                <  1:641127‑M2/100‑1 (MQ=255)
                                                                                                        atgatattaataattccactgcctttgttagaggaaagtcctaaATAATAATCAGTTGTTACATTATTGTTCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAcgttt                                           <  2:645359‑M2/105‑1 (MQ=255)
                                                                                                          gatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                         <  1:190581‑M2/107‑1 (MQ=255)
                                                                                                          gatattaataattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTAGTGTGCATTTCACTACTGGAACTGTAATCAAAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                         <  2:683152‑M2/107‑1 (MQ=255)
                                                                                                             attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                      <  1:334764‑M2/110‑1 (MQ=255)
                                                                                                             attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                      <  1:388306‑M2/110‑1 (MQ=255)
                                                                                                              ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                     >  1:368353‑M2/39‑149 (MQ=255)
                                                                                                              ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                     >  2:495966‑M2/39‑149 (MQ=255)
                                                                                                              ttattaattccactgcctttggtagagaaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                     <  1:848357‑M2/111‑1 (MQ=255)
                                                                                                                      tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTCTTTGATTAAATTGAAAGATGTTTGTTAAGGCATGGAt                             >  2:281494‑M2/31‑149 (MQ=255)
                                                                                                                          ctgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                         <  2:488744‑M2/123‑1 (MQ=255)
                                                                                                                           tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAATTTATTGTGTATTTCACTACTGGAACTGTAATCATAAAAGATTGACATGCTTAGCAAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                        <  1:407909‑M2/124‑1 (MQ=255)
                                                                                                                            gcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGc                       <  2:599396‑M2/125‑1 (MQ=255)
                                                                                                                                 tggtataggaagttgctaaATAATAATTAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAg                  <  2:361126‑M2/130‑1 (MQ=255)
                                                                                                                                  ggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGa                 <  1:333628‑M2/131‑1 (MQ=255)
                                                                                                                                   gtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAt                >  2:131532‑M2/18‑149 (MQ=255)
                                                                                                                                    tagaggaaagttctaaACAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAtt               <  1:414813‑M2/133‑1 (MQ=255)
                                                                                                                                      gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATAAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGATAGATGTTTGTTAAGGCATGGATGCAAGCTATCGGTTCt             >  2:366895‑M2/15‑149 (MQ=255)
                                                                                                                                                ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCa   >  2:563560‑M2/5‑149 (MQ=255)
                                                                                                                                                 taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGTATGCCAGCTATAGATTCTGATACGGTCaa  >  2:331618‑M2/4‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  NC_000913_3_pae_tpiA/1300550‑1300843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.