Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1299499–1300697	1300697	1–1199	62 [0]	[0] 62	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1299498	0 (0.000)	60 (1.270)	49/282	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_pae_tpiA	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  NC_000913_3_pae_tpiA/1300550‑1300843                                                                                                                                                     |                                                                                                                                                  ttctcaaattacagttagttataaggatttccttaactgattctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataatttaaatgatattattaattcaactgcctttggtagcggaaagtgctaaa                                                                                                                                                   >  2:25047‑M2/149‑149 (MQ=255)    tcaaaatacagttagttataaggatttccttaactgctactcctaaccatcaaattatattcgccacatcataatcctgggcatgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataa                                                                                                                                                <  2:32670‑M2/4‑1 (MQ=255)       aattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                             <  2:699265‑M2/7‑1 (MQ=255)             agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                       <  1:710750‑M2/13‑1 (MQ=255)                   ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                                 <  2:482227‑M2/19‑1 (MQ=255)                     ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgatggagaataagttaaatgatattattaattccactgcctttggtagtgaaaagtgctaaATAATAATCAATTGTTAAatt                                                                                                                               >  2:519237‑M2/129‑149 (MQ=255)                          gatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgt                                                                                                                          >  1:599396‑M2/124‑149 (MQ=255)                            tttccttaactgcttctcctcaccatcaagttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccagtgcctttggtagaggaaagtgctaaATAATACTAAATTCttaacatattattc                                                                                                                        >  2:345681‑M2/122‑145 (MQ=255)                              tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATAAATTGTTAAATTATTGTGCAt                                                                                                                      >  2:333628‑M2/120‑149 (MQ=255)                                cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaaagatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                    <  1:117615‑M2/32‑1 (MQ=255)                                    actgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCact                                                                                                                >  1:569309‑M2/114‑149 (MQ=255)                                       gcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                             <  1:60352‑M2/39‑1 (MQ=255)                                                       ctgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                             <  2:821035‑M2/55‑1 (MQ=255)                                                       atgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                             >  2:388306‑M2/95‑149 (MQ=255)                                                            attttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                        <  1:86152‑M2/60‑1 (MQ=255)                                                                   ccccacaataatcctgtgctcgctaaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                 <  2:164412‑M2/67‑1 (MQ=255)                                                                   ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                 >  1:217280‑M2/83‑149 (MQ=255)                                                                   ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                 >  1:595092‑M2/83‑149 (MQ=255)                                                                   ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTAATCTGCTGTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                 <  1:416172‑M2/67‑1 (MQ=255)                                                                    cagagcataatcctgggcttgctgaagaataattgaaatgatattatgaatgccactgactttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCGGTACTGGTACTGTGATCAGAAAAGATAGACATGc                                                                                <  2:191904‑M2/68‑1 (MQ=255)                                                                      catcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCtt                                                                              >  1:5077‑M2/80‑149 (MQ=255)                                                                        taataagcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATGGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAg                                                                            <  2:393774‑M2/72‑1 (MQ=255)                                                                          ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTTTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAACGATAGAAATGCTTAGcc                                                                          >  1:402747‑M2/76‑149 (MQ=255)                                                                          ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGcc                                                                          >  1:650435‑M2/76‑149 (MQ=255)                                                                              tcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtc                                                                      >  1:788472‑M2/72‑149 (MQ=255)                                                                                     cttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAttt                                                               <  2:650435‑M2/85‑1 (MQ=255)                                                                                         ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           <  1:653028‑M2/89‑1 (MQ=255)                                                                                         ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           >  2:351272‑M2/61‑149 (MQ=255)                                                                                         ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           <  1:384779‑M2/89‑1 (MQ=255)                                                                                           gaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgattga                                                         >  1:296666‑M2/59‑149 (MQ=255)                                                                                            aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                    <  2:208278‑M2/32‑1 (MQ=255)                                                                                            aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                    >  1:208278‑M2/58‑89 (MQ=255)                                                                                              gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATACTCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGCAAAGATAGACATGCTTCTCCACTCTCTATTTAattcaatt                                                      >  2:457778‑M2/56‑149 (MQ=255)                                                                                              gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaatt                                                      <  2:217280‑M2/94‑1 (MQ=255)                                                                                              gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaatt                                                      <  1:211948‑M2/94‑1 (MQ=255)                                                                                               aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtc                                                                      >  1:442223‑M2/55‑132 (MQ=255)                                                                                               aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtc                                                                      <  2:442223‑M2/78‑1 (MQ=255)                                                                                               aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattg                                                     >  2:427334‑M2/55‑149 (MQ=255)                                                                                                 taattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           >  2:658432‑M2/53‑141 (MQ=255)                                                                                                 taattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           <  1:658432‑M2/89‑1 (MQ=255)                                                                                                  aattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                                  <  2:361309‑M2/98‑1 (MQ=255)                                                                                                    ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                                >  2:820824‑M2/50‑149 (MQ=255)                                                                                                    ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                                <  1:641127‑M2/100‑1 (MQ=255)                                                                                                         atgatattaataattccactgcctttgttagaggaaagtcctaaATAATAATCAGTTGTTACATTATTGTTCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAcgttt                                           <  2:645359‑M2/105‑1 (MQ=255)                                                                                                           gatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                         <  1:190581‑M2/107‑1 (MQ=255)                                                                                                           gatattaataattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTAGTGTGCATTTCACTACTGGAACTGTAATCAAAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                         <  2:683152‑M2/107‑1 (MQ=255)                                                                                                              attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                      <  1:334764‑M2/110‑1 (MQ=255)                                                                                                              attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                      <  1:388306‑M2/110‑1 (MQ=255)                                                                                                               ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                     >  1:368353‑M2/39‑149 (MQ=255)                                                                                                               ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                     >  2:495966‑M2/39‑149 (MQ=255)                                                                                                               ttattaattccactgcctttggtagagaaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                     <  1:848357‑M2/111‑1 (MQ=255)                                                                                                                       tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTCTTTGATTAAATTGAAAGATGTTTGTTAAGGCATGGAt                             >  2:281494‑M2/31‑149 (MQ=255)                                                                                                                           ctgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                         <  2:488744‑M2/123‑1 (MQ=255)                                                                                                                            tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAATTTATTGTGTATTTCACTACTGGAACTGTAATCATAAAAGATTGACATGCTTAGCAAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                        <  1:407909‑M2/124‑1 (MQ=255)                                                                                                                             gcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGc                       <  2:599396‑M2/125‑1 (MQ=255)                                                                                                                                  tggtataggaagttgctaaATAATAATTAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAg                  <  2:361126‑M2/130‑1 (MQ=255)                                                                                                                                   ggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGa                 <  1:333628‑M2/131‑1 (MQ=255)                                                                                                                                    gtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAt                >  2:131532‑M2/18‑149 (MQ=255)                                                                                                                                     tagaggaaagttctaaACAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAtt               <  1:414813‑M2/133‑1 (MQ=255)                                                                                                                                       gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATAAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGATAGATGTTTGTTAAGGCATGGATGCAAGCTATCGGTTCt             >  2:366895‑M2/15‑149 (MQ=255)                                                                                                                                                 ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCa   >  2:563560‑M2/5‑149 (MQ=255)                                                                                                                                                  taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGTATGCCAGCTATAGATTCTGATACGGTCaa  >  2:331618‑M2/4‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  NC_000913_3_pae_tpiA/1300550‑1300843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑